Rabbit Anti-Human TRIM28 (N-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q13263 |
| Gene Id | 10155 |
| Gene Symbols | TRIM28 |
| Protein Name / Synonyms | TRIM28 (N-term) |
| Clonality | Polyclonal |
| Preparation | This TRIM28 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 178-207 amino acids from the N-terminal region of human TRIM28. |
| Concentration (lot specific) | 0.470 mg/ml |
| Recommended Applications | Western Blotting, IHC-P, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
The protein encoded by this gene mediates transcriptional control by interaction with the Kruppel-associated box repression domain found in many transcription factors. The protein localizes to the nucleus and is thought to associate with specific chromatin regions. The protein is a member of the tripartite motif family. This tripartite motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region.
Specificity
Other Names
TRIM28; KAP1; RNF96; TIF1B; Transcription intermediary factor 1-beta; E3 SUMO-protein ligase TRIM28; KRAB-associated protein 1; KRAB-interacting protein 1; Nuclear corepressor KAP-1; RING finger protein 96; Tripartite motif-containing protein 28
NCBI Accession #
NP_005753.1
Other Accession #
NP_005753.1
Format
Type
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
88550
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; IF: 1:10-50
 | TRIM28 Antibody (N-term) (Cat# 102-11288) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow). |
 | TRIM28 Antibody (N-term) (Cat# 102-11288)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM28 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated. |
 | Fluorescent confocal image of Hela cell stained with TRIM28 Antibody (N-term)(Cat#RB09760).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.