This product recognizes the alpha subunit of tubulin, specifically binding tyrosylated Tubulin (Tyr-Tubulin), see Wehland, J. et al. for details. The epitope recognized by this antibody has been extensively studied and would appear to be a linear sequence requiring an aromatic residue at the C terminus, with the two adjacent amino acids being negatively charged (represented by Glu-Glu-Tyr in Tyr-Tubulin).

The antibody has been used in epitope tagging procedures to detect proteins tagged with a C-terminal Gly-Gly-Phe epitope. These sequence requirements have been reported to result in some cross-reactivity with other proteins in certain circumstances, including E. coli rec A and oxidized actin, see Burns, R. for details.

This product is routinely tested in ELISA on Tubulin.

IgG concentration 1.0 mg/ml