Rabbit Anti-Human U2AF1 (Center Y114) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | Q01081 |
| Gene Id | 7307 |
| Gene Symbols | U2AF1 |
| Protein Name / Synonyms | U2AF1 (Center Y114) |
| Clonality | Polyclonal |
| Preparation | This U2AF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-125 amino acids from the Central region of human U2AF1. |
| Concentration (lot specific) | 2.0 mg/ml |
| Recommended Applications | FC, IHC-P, Western Blotting |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
U2AF1 belongs to the splicing factor SR family. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers.
Specificity
Predicted Reactivity
Mouse, Bovine
Other Names
U2AF1; U2AF35; U2AFBP; Splicing factor U2AF 35 kDa subunit; U2 auxiliary factor 35 kDa subunit; U2 small nuclear RNA auxiliary factor 1; U2 snRNP auxiliary factor small subunit
NCBI Accession #
NP_001020374.1;NP_001020375.1;NP_006749.1
Other Accession #
Q9D883; A1A4K8
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
27872
Recommended Dilutions
WB: 1:1000; IHC: 1:50-100; FC: 1:10-50
 | Western blot analysis of anti-U2AF1 Antibody (Center Y114) (Cat# 102-15150) in Ramos cell line lysates (35ug/lane).U2AF1(arrow) was detected using the purified Pab. |
 | Formalin-fixed and paraffin-embedded human lung carcinoma reacted with U2AF1 Antibody (Center Y114) (Cat# 102-15150), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. |
 | U2AF1 Antibody (Center Y114) (Cat# 102-15150) flow cytometry analysis of Ramos cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis. |
 | Western blot analysis of U2AF1 (arrow) using rabbit polyclonal U2AF1 Antibody (Center Y114) (Cat# 102-15150). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the U2AF1 gene. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.