Rabbit Anti-Human ZBTB7B (C-term) Antibody, 400 ul
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Product Description
Specifications
| Size | 400 µL |
| Estimated Lead Time | 1-2 weeks |
| Species | Human |
| Host Species | Rabbit |
| Accession Number | O15156 |
| Gene Id | 51043 |
| Gene Symbols | ZBTB7B |
| Protein Name / Synonyms | ZBTB7B (C-term) |
| Clonality | Polyclonal |
| Preparation | This ZBTB7B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 440-469 amino acids from the C-terminal region of human ZBTB7B. |
| Concentration (lot specific) | 2.000 mg/ml |
| Recommended Applications | Western Blotting, IF |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
ZBTB7B is a transcription regulator that acts as a key regulator of lineage commitment of immature T-cell precursors. It is necessary and sufficient for commitment of CD4 lineage, while its absence causes CD8 commitment. Development of immature T-cell precursors (thymocytes) to either the CD4 helper or CD8 killer T-cell lineages correlates precisely with their T-cell receptor specificity for major histocompatibility complex class II or class I molecules, respectively. ZBTB7B is a transcriptional repressor of the collagen COL1A1 and COL1A2 genes. It may also function as a repressor of fibronectin and possibly other extracellular matrix genes.
Specificity
Other Names
ZBTB7B; ZBTB15; ZFP67; ZNF857B; Zinc finger and BTB domain-containing protein 7B; Krueppel-related zinc finger protein cKrox; T-helper-inducing POZ/Krueppel-like factor; Zinc finger and BTB domain-containing protein 15; Zinc finger protein 67 homolog; Zinc finger protein 857B; Zinc finger protein Th-POK
NCBI Accession #
NP_001239335.1;NP_001243384.1
Format
Type
Purified Rabbit Polyclonal Antibody (Pab)
Calculated Molecular Weight (Da)
58027
Recommended Dilutions
WB: 1:1000; IF: 1:10-50
 | Western blot analysis of ZBTB7B Antibody (C-term) (Cat# 102-16762) in 293 cell line lysates (35ug/lane). ZBTB7B (arrow) was detected using the purified Pab. |
 | Fluorescent confocal image of Hela cell stained with ZBTB7B Antibody (C-term)(Cat# 102-16762).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ZBTB7B primary antibody (1:25, 1 h at 37°). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37°). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). ZBTB7B immunoreactivity is localized to Nucleus significantly. |
Antigen Source
HUMAN
Storage/Stability
2-8°C (short-term); -20°C (long-term)
Expiration:
12 months from the date of shipment when stored properly.