Human Angiogenesis Array C1000

RayBio® C-Series Human Angiogenesis Antibody Array 1000 Kit. A combination of Human Angiogenesis Antibody Array C1 & C2. Detects 43 Human Angiogenic Factors. Suitable for all liquid sample types.


Assay Format

Number of Targets Detected:
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
  • Semi-Quantitative
Solid Support:

Product Specifications

4 or 8 Sample Kit

Protein Information

Gene Symbols:
ANG | ANGPT1 | ANGPT2 | CCL1 | CCL13 | CCL2 | CCL5 | CCL7 | COL18A1 | CSF2   View more

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
GRO alpha/beta/gamma
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
TGF beta 1
TNF alpha

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Angiogenesis Antibody Array C1000 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis


For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Greenberger S., Boscolo E., Adinit I., et al., Corticosteroid suppression of VEGF-A in infantile hemangioma-derived stem cells. N Engl J Med. 2010 Mar 18;362(11):1005-13. doi: 10.1056/NEJMoa0903036.
    Species: Human
    Sample type: Conditioned Media (Hemangioma derived Stem Cells)
  2. Battinelli, Elisabeth M., Beth A. Markens, and Joseph E. Italiano. "Release of angiogenesis regulatory proteins from platelet alpha granules: modulation of physiologic and pathologic angiogenesis." Blood 118.5 (2011): 1359-1369.
    Species: Human
    Sample type: Conditioned Media (platelets)
  3. Zhu L., Cheng X., Shi J., et al. Crosstalk between bone marrow-derived myofibroblasts and gastric cancer cells regulates cancer stemness and promotes tumorigenesis. Oncogene advance online publication 25 April 2016; doi: 10.1038/onc.2016.76
    Species: Mouse
    Sample type: Conditioned Media (Bone Marrow fibroblasts and MKN28 gastric cancer cell lines)
  4. Kim, Eun Ju, et al. "UV modulation of subcutaneous fat metabolism." Journal of Investigative Dermatology 131.8 (2011): 1720-1726.
    Species: Human
    Sample type: Conditioned Media (keratinocytes, fibroblasts)
  5. Rezzola S., Corsini M., Chiodelli P., et al. Inflammation and N-formyl peptide receptors mediate the angiogenic activity of human vitreous humour in proliferative diabetic retinopathy. Diabetologia. 2017 Apr;60(4):719-728. doi: 10.1007/s00125-016-4204-0.
    Species: Human
    Sample type: Vitreous Fluid (Proliferative diabetic retinopathy)
  6. Chung E., Rytlewski J., Merchant AG., et al. Fibrin-based 3D matrices induce angiogenic behavior of adipose-derived stem cells. Acta Biomaterialia Available online 16 January 2015. doi:10.1016/j.actbio.2015.01.012
    Species: Human
    Sample type: Conditioned Media (Adipose-derived stem celsl (ACSs))
  7. Hossain A., Gumin J., Gao F., et al. Mesenchymal Stem Cells Isolated From Human Gliomas Increase Proliferation and Maintain Stemness of Glioma Stem Cells Through the IL-6/gp130/STAT3 Pathway. Stem Cells. 2015 Aug;33(8):2400-15. doi: 10.1002/stem.2053.
    Species: Human
    Sample type: Conditioned Media (Mesenchymal cells in gliomas help maintain "stemness" in gioma cells)
  8. Jin YP., Hu YP., Wu XS., et al. miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma. Cell Death Dis. 2018 Feb; 9(2): 182. 2018 Feb 7. doi: 10.1038/s41419-017-0258-2
    Species: Human
    Sample type: Conditioned Media (Gall bladder cancner cell line, NOZ treated with microRNAs)
  9. Jin, YP., Hu, YP., Wu, XS. et al. miR-143-3p targeting of ITGA6 suppresses tumour growth and angiogenesis by downregulating PLGF expression via the PI3K/AKT pathway in gallbladder carcinoma. Cell Death Dis 9, 182 (2018).
    Species: Human
    Sample type: Conditioned media (cell culture media)
  10. Unterman S., Freiman A., Beckerman M., et al. Tuning of collagen scaffold properties modulates embedded endothelial cell regulatory phenotype in repair of vascular injuries in vivo. Adv Healthc Mater. 2015 Oct 28;4(15):2220-8. doi: 10.1002/adhm.201500457.
    Species: Human
    Sample type: Conditioned Media (Matrix embedded endothelial cells)
I was skeptical if this antibody array will work with my experiment as I had less sample quantity. I used 150 ug of total protein from Human endothelial cell lysate and followed overnight incubation protocol and got a good signal. The procedure was easy to follow and included tray made handling a lot easier.

I ordered the Angiogenesis Array C1000 last week and it worked so great!! The protocol is super straight forward and the results very clear. I will be trying other arrays in the future. I highly recommend this product!

Yale University
I used the array for measuring angiogenesis-related cytokine in the cell culture supernatant . It is a good product.

Florida A&M University
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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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