Human RTK Phosphorylation Array C1

$363.00
AAH-PRTK-1-2

RayBio® C-Series Human Receptor Tyrosine Kinase Phosphorylation Antibody Array 1 Kit. Detects 71 Human RTKs. Suitable for all liquid sample types but intended for use with cell and tissue lysates.

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Antigen Information

Gene Symbol:
  • ABL1
  • AXL
  • BLK
  • BMX
  • BTK
  • CSF1R
  • CSK
  • DDR2
  • EGFR
  • EPHA1
  • EPHA2
  • EPHA3
  • EPHA4
  • EPHA5
  • EPHA6
  • EPHA7
  • EPHA8
  • EPHB1
  • EPHB2
  • EPHB3
  • EPHB4
  • EPHB6
  • ERBB2
  • ERBB3
  • ERBB4
  • FER
  • FGFR1
  • FGFR2
  • FGR
  • FLT4
  • FRK
  • FYN
  • HCK
  • IGF1R
  • INSR
  • ITK
  • JAK1
  • JAK2
  • JAK3
  • KDR
  • KIT
  • LCK
  • LTK
  • LYN
  • MATK
  • MET
  • MUSK
  • NGFR
  • NTRK2
  • PDGFRA
  • PDGFRB
  • PTK2
  • PTK2B
  • RET
  • ROR1
  • ROR2
  • ROS1
  • RYK
  • SLPI
  • SRMS
  • SYK
  • TEC
  • TEK
  • TIE1
  • TNK1
  • TNK2
  • TXK
  • TYK2
  • TYRO3
  • ZAP70

Assay Format

Pathway:
  • PI3K-AKT Signaling
Number of Targets Detected:
71
Species Detected:
  • Human
Compatible Sample Types:
  • Serum
  • Plasma
  • Cell Culture Supernatants
  • Cell Lysates
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
ABL1
  • Gene Symbol: ABL1
  • Gene ID: 25
  • Accession #: P00519
ACK
ALK-1
Axl
Blk
BMX
Btk
Csk
Dtk
EGFR
EphA1
EphA2
EphA3
EphA4
EphA5
EphA6
EphA7
EphA8
EphB1
EphB2
EphB3
EphB4
EphB6
ErbB2
ErbB3
ErbB4
FAK
FER
FGFR1
FGFR2
FGFR2 alpha isoform
  • Gene Symbol: FGFR2
  • Gene ID: 2263
  • Accession #: P21802
Fgr
FRK
Fyn
Hck
HGFR
IGF-1 R
Insulin R
(CD220)
Itk
JAK1
JAK2
JAK3
LCK
LTK
Lyn
MATK
M-CSF R
MUSK
NGFR
(TNFRSF16)
PDGFRA
PDGFRB
PYK2
RET
ROR1
ROR2
ROS
RYK
SCF R
(CD117/c-kit)
SRMS
SYK
Tec
Tie-1
Tie-2
TNK1
TRKB
TXK
Tyk2
TYRO10
(DDR2/TKT)
VEGFR2
VEGFR3
ZAP70

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human RTK Phosphorylation Antibody Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Anti-Phospho Tyrosine Detection Antibody
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer (1 x 5 ml)
  • Protease Inhibitor Cocktail
  • Phosphatase Inhibitor Cocktail II
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Wang Z., Gao Q., Shi J., et al. Protein tyrosine phosphatase receptor S acts as a metastatic suppressor in hepatocellular carcinoma by control of epithermal growth factor receptor-induced epithelial-mesenchymal transition. Hepatology. 2015 Oct;62(4):1201-14. doi: 10.1002/hep.27911.
    Species: Human
    Sample type: Cell Lysate
  2. Cortelazzi B., Verderio P., Ciniselli CM., et al. Receptor tyrosine kinase profiles and human papillomavirus status in oropharyngeal squamous cell carcinoma.J Oral Pathol Med. 2014 Dec 14. doi: 10.1111/jop.12301. Epub ahead of print
    Species: Human
    Sample type: Tissue Lysate
  3. Acquired resistance to sunitinib in human renal cell carcinoma cells is mediated by constitutive activation of signal transduction pathways associated with tumour cell proliferation
    Species: Human
    Sample type: Conditioned Media
  4. Al-aidaroos A., et al. Metastasis-associated PRL-3 induces EGFR activation and addiction in cancer cells. J Clin Invest. 2013;123(8):3459-3471. doi:10.1172/JCI66824.
    Species: Human
    Sample type: Cell Lysate
  5. Siu IM., et al. Erlotinib Inhibits Growth of a Patient-Derived Chordoma Xenograft . Published: November 15, 2013 DOI: 10.1371/journal.pone.0078895
    Species: Human
    Sample type: Cell Lysate
  6. Guerra-Laso, José M., et al. "Macrophages from elders are more permissive to intracellular multiplication of Mycobacterium tuberculosis." Age 35.4 (2013): 1235-1250.
    Species: Human
    Sample type: Cell Lysate
  7. Tirado-González, I., et al. "Galectin-1 influences trophoblast immune evasion and emerges as a predictive factor for the outcome of pregnancy." Molecular human reproduction 19.1 (2013): 43.
    Species: Human
    Sample type: Cell Lysate
  8. Kato K., Gong J., Iwama H., et al. The antidiabetic drug metformin inhibits gastric cancer cell proliferation in vitro and in vivo. Mol Cancer Ther. 2012 Mar;11(3):549-60. doi: 10.1158/1535-7163.MCT-11-0594
    Species: Human
    Sample type: Cell Lysate
  9. Li J, Sipple J, Maynard S, et al. Fanconi Anemia Links Reactive Oxygen Species to Insulin Resistance and Obesity. Antioxidants & Redox Signaling 2012;17(8):1083-1098. doi:10.1089/ars.2011.4417.
    Species: Human
    Sample type: Cell Lysate
  10. Shimokawa T, Seike M, Soeno C, et al. Enzastaurin has anti-tumour effects in lung cancers with overexpressed JAK pathway molecules. British Journal of Cancer 2012;106(5):867-875. doi:10.1038/bjc.2012.7.
    Species: Human
    Sample type: Cell Lysate

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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