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Fighting Coronavirus

With the global outbreak of the novel SARS-like coronavirus, SARS-CoV-2 (2019-nCoV, COVID-19 virus) early diagnostics and infection prevention and control has become critical. Early detection leading to quarantine can significantly impact the ability of local governments to limit rapid spread to at risk patients. Scientists identified the novel coronavirus as a group 2B CoV, distinct from the SARS-CoV [1-3]. Genome sequencing indicated that SARS-CoV-2 (2019-nCoV) shares 79.5% sequence identity with SARS-CoV and 96% of identity with a bat coronavirus [4].

RayBiotech provides the most comprehensive technologies, tools, and products for COVID-19 clinical diagnosis, research and detection:

Antibody Testing

COVID-19 IgM/IgG Rapid Test Kit for Clinical Diagnosis

10 Minutes Rapid Detection of SARS-CoV-2 (COVID-19) N-Protein Antibodies

RayBiotech offers lateral flow devices for the detection of IgG and IgM antibodies to the coronavirus N-protein in serum, plasma, and peripheral blood. An example of a lateral flow device is a home pregnancy test. They are easy to use and provide rapid results.

COVID-19 Rapid Test Kit
Coronavirus IgG detection strip

This rapid test is for in vitro diagnostic use, following guidance from the FDA for Emergency Use Authorizations of tests submitted for approval on March 16, 2020. The tests have not been reviewed by the FDA and results from antibody testing should not be used as the sole basis to diagnose or exclude SARS-CoV-2 (COVID-19) infection or to inform infection status.

IgG/IgA/IgM Antibody Detection Kit (Indirect ELISA)

N-Protein and S-Protein (S1 subunit, RBD) human antibody detection kits (Indirect ELISA method) for the semi-quantitative measurement of human IgG, IgA, or IgM antibody against SARS-CoV-2 in serum or plasma.
N-Protein S-Protein (S1RBD)
COVID-19 Indirect ELISA

Proteins

The spike protein (S-protein) and nucleocapsid protein (N-protein) are encoded by all coronaviruses, including the coronavirus (COVID-19) that was first detected in Wuhan City, China, in December 2019. Both recombinant forms of these proteins are now available from RayBiotech to advance infectious disease research.

COVID-19 Proteins
COVID-19 Proteins SDS-PAGE

Antibodies

Polyclonal antibodies to SARS-CoV-2.

COVID-19 Antibodies
COVID-19 Antibodies

PCR

SARS-CoV-2 High Throughput RT-PCR Nucleic Acid Detection Kit

80 tests. Suitable for the qualitative in vitro detection of novel coronavirus (SARS-CoV-2 / COVID-19) nucleic acid in patient sera and respiratory specimens (including pharyngeal swabs and alveolar lavage fluid samples).

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COVID-19 PCR

SARS-CoV-2 Real Time RT-PCR Nucleic Acid Detection Kit

20 tests. Suitable for the qualitative detection of new coronavirus (SARS-CoV-2 / COVID-19) nucleocapsid protein N gene in human throat swabs and alveolar lavage. Includes a one-step RT-qPCR mix. Conforms to CDC guidelines. LDT ready for high complexity CLIA labs.

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Coronavirus PCR Kit

Serum & Plasma Samples for COVID-19 Research

Serum and plasma (EDTA) from normal (non-COVID-19) and COVID-19 patients.

COVID-19 Sample COVID-19 Sample Set (30 patients)
Normal Sample (negative for COVID-19)
COVID-19 Spike-ACE2 binding assay

Binding Assay

COVID-19 Spike-ACE2 Binding Assay Kit

The entry of the SARS-CoV-2 virus into host cell is mediated by an interaction between the Spike (S) protein’s receptor binding domain (RBD) on the surface of the viral particle and the Angiotensin I Converting Enzyme 2 (ACE2) receptor on the human cell surface. As such, this binding event is a biological target for COVID-19 therapies. The RayBio® COVID-19 Spike-ACE2 binding assay kit allows the researcher to rapidly screen and identify small molecules, antibodies, or other biological molecules that potentially interfere with the formation of the S-ACE2 complex.

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COVID-19 Spike-ACE2 binding assay

ELISA Kits

COVID-19 N-Protein Human IgG, IgA, or IgM ELISA Kit - Semi-quantitative measurement of human IgG, IgA, or IgM antibody against SARS-CoV-2 N-Protein in serum or plasma sample types.

COVID-19 S-Protein (S1RBD) Human IgG, IgA, or IgM ELISA Kit - Semi-quantitative measurement of human IgG, IgA, or IgM antibody against SARS-CoV-2 S-Protein S1 subunit receptor binding domain (RBD) in serum or plasma sample types.

COVID-19 N-Protein ELISA - SARS-CoV-2 Nucleocapsid Protein ELISA Kit. Detects the N-Protein in serum, plasma, and cell culture supernatant sample types.

COVID-19 S-Protein (S2 subunit) ELISA - SARS-CoV-2 Spike Protein ELISA Kit. Detects the S-Protein (S2 subunit) in serum, plasma, and cell culture supernatant sample types.

Human ACE-2 ELISA - Human ACE-2 (angiotensin-converting enzyme 2) protein can be secreted but is also present as an integral membrane protein that serves as the initial attachment point for SARS-CoV and SARS-CoV-2 [5,6]. All coronavirus strains have a spike protein (S-protein) that mediates viral entry into the cell via interaction with ACE-2. Soluble ACE-2 has been shown to block the binding of SARS-CoV S-protein, potentially slowing down viral replication [5].

COVID-19 ELISA

Multiplex Protein Array

Semi-quantitative detection of IgM and IgG antibodies in serum or plasma to the SARS-CoV-2 (COVID-19) spike (S) protein.

COVID-19 Spike Protein Array
COVID-19 Array

Inflammation Cytokine Arrays

During infection of the coronavirus COVID-19, high levels of inflammatory cytokines have been reported. These cytokines include interferons, interleukins, chemokines, colony-stimulating factors, and tumor necrosis factors and contribute to the symptoms of coronavirus infection: redness, swelling/edema, fever, and pain. Overproduction of pro-inflammatory cytokines can result in a “cytokine storm,” during which inflammation spreads throughout the body via the circulation. One common consequence of a cytokine storm is acute lung injury, which can progress to a more severe form called acute respiratory distress syndrome. The dynamic relationship of cytokines during inflammation can be measured using RayBiotech’s quantitative inflammation antibody arrays below. These Quantibody® arrays are a multiplex ELISA platform suitable for all liquid sample types, providing quantitative data (i.e., concentrations) of your proteins-of-interest.

Array Number of Targets Targets Detected
Human Inflammation Array Q1 10 IL-1 alpha, IL-1 beta, IL-4, IL-6, IL-8, IL-10, IL-13, MCP-1, IFN-gamma, TNF alpha
Human Inflammation Array Q3 40 BLC, Eotaxin-1, Eotaxin-2, G-CSF, GM-CSF, I-309, ICAM-1, IFN-gamma, IL-1 alpha, IL-1 beta, IL-1 ra, IL-2, IL-4, IL-5, IL-6, IL-6 sR, IL-7, IL-8, IL-10, IL-11, IL-12 p40, IL-12 p70, IL-13, IL-15, IL-16, IL-17, MCP-1, M-CSF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PDGF-BB, RANTES, TIMP-1, TIMP-2, TNF alpha, TNF beta, sTNFRI, sTNFRII
Human Th1/Th2 Array Q1 10 GM-CSF, IFN-gamma, IL-10, IL-13, IL-2, IL-4, IL-5, IL-6, IL-8 (CXCL8), TNF alpha

Small Molecules / Inhibitors

Name Catalog # CAS # Target Candidate
Arbidol HCl 331-11756 131707-23-8 Viral spike glycoprotein for binding to host cell receptor ACE2
Ritonavir 331-11642 155213-67-5 HIV Protease
Lopinavir 331-11643 192725-17-0 Coronavirus main protease 3CLpro
Darunavir 331-11645 206361-99-1 HIV Protease
Ribavirin 331-10301 36791-04-5 RNA-dependent RNA polymerase
Chloroquine diphosphate 331-11962 54-05-7 Malaria (endosome/ACE2)
Nitazoxanide 331-11871 55981-09-4 Viral protein expression
Camostat mesilate 332-10779 59721-29-8 Transmembrane protease, serine 2, primes S protein to facilitate its binding to ACE2
Hydroxychloroquine Sulfate 331-21357 747-36-4 Malaria (endosome/ACE2)
Baricitinib Phosphate 332-10769 1187595-84-1 JAK kinase that regulate the inflammatory processes

Biomarker Discovery & Protein Profiling

In the fight against coronavirus COVID-19, protein profiling can be used to understand the host response to infection and identify potential biomarkers for drug development. RayBiotech offers full testing services to measure as many as 1200 human proteins simultaneously using our quantitative Quantibody® antibody array platform.

Questions? Please contact [email protected] or at (888) 494-8555.

References:

  1. Virological.org. Novel 2019 Coronavirus Genome.[view] (accessed on 28 January 2020).
  2. GSAID Database. 2020 Coronavirus.[view] (accessed on 28 January 2020).
  3. Severe acute respiratory syndrome coronavirus 2 isolate Wuhan-Hu-1, complete genome. [view]
  4. Shi, Z.L., et al. Discovery of a novel coronavirus associated with the recent pneumonia outbreak in humans and its potential bat origin. bioRxiv 2020.
  5. Li, W. et al. (2003) Nature 426:450.
  6. Hoffmann, M. et al. (2020) Cell. DOI: 10.1016/j.cell.2020.02.052.