Overall this kit was very easy to use and offered reliable, replicable results. The modular test strips made for easy testing setups without having to commit to running a full 96 wells, which allowed us to do easy preliminary testing while still getting used to the kit. Our only complaint is that it doesn't last longer. Would love to see the development of a more stable kit. Overall this kit perfectly suited our needs and we will most definitely be ordering more in the future.

Review by Ed, on Apr 3, 2023

We followed the exact protocol on from the manual. The kit is easy to use. Gives rapid results.

Review by Ishan Soni, from Leveragen, Inc., on Mar 18, 2023

The kit is easy to use and reliable. We have generated lots of data using this kit. I like this particular kit (CoV-ACE2S2-1) because the RBD is Fc-Tagged allowing to test the effects of diverse agents even spike proteins.

Review by Justin, from Georgetown University, on Feb 22, 2023

Positive feed back from our R&D team.

Review by Destiny, from AIVITA Biomedical, on Sep 11, 2022

The kit is great, we have use 3 kits already. Something extra to say not product related itself, is BE CAREFUL with the temperature labeled in the shipping box. All items I've got from RayBiotech has came with a -20C storage recommendation when the actual kit temp is 4C and few items are -20. My coworker didnt check and all my kit got into -20 overnight until I came next day and verify it.

Review by John S., from Stanford University, on May 16, 2021

Kit doesn't come with kind of protocol but, works very well.

Review by Nitin, from The University of Cincinnati, on Apr 28, 2021

Some of the wells did work well. Next time we are planning to do an overnight incubation to give it a better chance. I'm ordering a 2nd kit.

Review by John S., from Stanford University, on Apr 20, 2021

we tested the transduction efficiency of the viruses on wild type 293 cells... plasma samples were used as negative.

Review by D Yun, from NANOBST, on Mar 16, 2021

I checked inhibition of SARS-CoV2 spike protein and ACE2 interaction by using using our developed vaccine and it worked really well.

Review by Ananya, from Washington University in St. Louis, on Mar 14, 2021

Kit helped us to check whether the our test compound is inhibiting the interaction or not

Review by Raj Nandani, from South dakota state university, on Mar 9, 2021

Positive control along with kit showed a good signal. However, our RBD antigen showed no binding with secondary Abs. So finally we used competitive ELISA, it looks good though. Now it is the 2nd kit we are using, good product.

Review by Haiyue Xu, from University of Texas at Austin, on Jan 31, 2021

Very easy to use, data are reliable and repeatable.

Review by Leo, from LSU, on Oct 28, 2020

Great kit at an affordable cost. Easy to use. Have the option to choose Spike or ACE 2 on plate. Overnight incubation yields very high OD at 450nm, but the incubation time could be adjusted to 1-2hours at RT. It would be beneficial if they could provide the concentration of 1X ACE2 protein and also could have included a positive control (a known inhibitor) in the kit.

Review by Prema, from University of Mississippi, on Oct 4, 2020

Instructions are easy to follow. Works well with both kit-provided and our own RBD protein. Only suggestion is they should at least disclose the concentration of the query protein.

Review by Eli, from Scripps Research, on Sep 1, 2020

The kit works as expected. Easy to use.

Review by KS, from National Institutes of Health Clinical Center, on Aug 27, 2020

I ordered the kit when there was only one option, "Spike RBD on plate + ACE2 query (CoV-SACE2)". At that time, the storage condition was -20°C on arrival, keeping some at -20°C and some at 4°C. I mentioned this for we should take care of the storage of the components of the kit.

Review by Chao, from UMichigan, on Aug 3, 2020

We obtained good results using this, so I'll be purchasing more. With that said, the instructions could have been written a bit clearer and the actual starting amount of ACE2 in vial F should be provided (phone operator said it was "unknown"). This makes assigning true values to the standard curve impossible. But as I said, the qualitative use for binding inhibition assays seems to work just fine, just can't be quantitative.

Review by Todd Wyatt, from University of Nebraska Medical Center, on Jun 11, 2020

The Spike-ACE2 binding assay is a sensitive and very suitable method for fast screening of potential inhibitors of the S-ACE2 complex formation. Signal intensity at 450 nm recorded for technical replicates results in very similar measurements and the signal-to-noise ratio is very high.

Review by MARINA K, from Pharma, on Jun 2, 2020

Great kit. Easy to use. I love how the rows of wells can pop out of the plate so the unused ones can remain in the fridge.

Review by Rose Gordon-Schneider, from Columbia University, on Jun 2, 2020