Human Cytokine Array C5

The Cytokine Array C5 is a membrane-based antibody array for screening and comparing expression levels of 80 human cytokines in any liquid sample type.

$440.36  
AAH-CYT-5-2
+

Assay Format

Number of Targets Detected:
80
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Chemiluminescence
Quantitative/Semi-Quantitative:
  • Semi-Quantitative
Solid Support:
Membrane

Product Specifications

Size:
2, 4, or 8 Sample Kit

Protein Information

Gene Symbols:
ANG | BDNF | CCL1 | CCL11 | CCL13 | CCL15 | CCL17 | CCL18 | CCL2 | CCL20   View more

Introduction

The cytokine array is an antibody based immunoassay similar to ELISA, but able to simultaneously measure multiple proteins on one solid support. The Cytokine Array C5 is a membrane-based antibody array, using a panel of 80 different capture antibodies printed on a nitrocellulose membrane. The protein from the incubated sample is captured, and a detection antibody is also bound, creating the antibody-antigen-antibody sandwich. C-Series arrays are processed similarly to a western blot and the entire procedure can be completed in 1 day with little to no optimization.

Learn More About Cytokine Arrays

Product Features

  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology (many publications)
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Angiogenin
BDNF
BLC
(CXCL13)
CK beta 8-1
(CCL23)
EGF
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GM-CSF
GRO alpha
(CXCL1)
GRO alpha/beta/gamma
HGF
I-309
(TCA-3/CCL1)
IFN-gamma
IGF-1
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-2
IL-3
IL-4
IL-5
IL-6
IL-7
IL-8
(CXCL8)
IL-10
IL-12 p40/p70
  • Gene Symbol: IL12A NKSF1
  • Gene ID: 3592
  • Accession #: P29459
IL-13
IL-15
IL-16
IP-10
(CXCL10)
Leptin
LIF
LIGHT
(TNFSF14)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
NAP-2
(PPBP/CXCL7)
NT-3
NT-4
Oncostatin M
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-BB
PLGF
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
TARC
(CCL17)
TGF beta 1
TGF beta 2
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
Thrombopoietin
(TPO)
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
VEGF-A

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
  • Human Cytokine Antibody Array C5 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates
  • Manual
Other Materials Required
  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
  1. Block membranes
  2. Incubate with Sample
  3. Incubate with Biotinylated Detection Antibody Cocktail
  4. Incubate with HRP-Conjugated Streptavidin
  5. Incubate with Detection Buffers
  6. Image with chemiluminescent imaging system
  7. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Acosta JC, O'Loghlen A, Banito A, Guijarro MV, Augert A, et al. Chemokine Signaling via the CXCR2 Receptor Reinforces Senescence. Cell. 2008;133:1006-1018.
    Species: Human
    Sample type: Conditioned Media (Fibroblast cell-cultured medium)
  2. McAllister SS, Gifford AM, Greiner AL, Kelleher SP, Saelzer MP, et al. Systemic Endocrine Instigation of Indolent Tumor Growth Requires Osteopontin. Cell. 2008;133:994-1005.
    Species: Human
    Sample type: Plasma (Plasma from mice with human tumor xenografts)
  3. Kim M, Oskarsson T, Acharyya S, Nguyen D, Zhang X, et al.Tumor Self-Seeding by Circulating Cancer Cells. Cell. 2009;139(7):1315-1326.
    Species: Human
    Sample type: Conditioned Media (Cell-conditioned media)
  4. Su S., Liu Q., Chen Jin., et al. A Positive Feedback Loop between Mesenchymal-like Cancer Cells and Macrophages Is Essential to Breast Cancer Metastasis. Cancer Cell. 2014 May 12;25(5):605-20. doi: 10.1016/j.ccr.2014.03.021.
    Species: Human
    Sample type: Conditioned Media (Mesenchymal-like Cancer cells)
  5. Vermeulen L., De Sousa E Melo F., van der Heijden M., et al. Wnt activity defines colon cancer stem cells and is regulated by the microenvironment. Nature Cell Biology 12, 468 - 476 (2010); doi:10.1038/ncb2048
    Species: Human
    Sample type: Conditioned Media (Myofibroblasts conditioned media)
  6. Acosta, Juan Carlos, et al. "A complex secretory program orchestrated by the inflammasome controls paracrine senescence." Nature cell biology 15.8 (2013): 978.
    Species: Human
    Sample type: Conditioned Media (senescene model - HEK293T and IMR90 cells)
  7. Pislaru SV, Harbuzariu A, Gulati R, Witt T, et al. Magnetically Targeted Endothelial Cell Localization in Stented Vessels. J Am Coll Cardiol. 2006; 48(9):1839-1845.
    Species: Human
    Sample type: Conditioned Media (Endothelial cell-conditioned medium)
  8. Trian T., Allard B., Dupin I., et al. House dust mites induce proliferation of severe asthmatic smooth muscle cells via an epithelium-dependent pathway. Am J Respir Crit Care Med. 2015 Mar 1;191(5):538-46. doi: 10.1164/rccm.201409-1582OC
    Species: Human
    Sample type: Conditioned Media (Bronchial epithelial cells)
  9. Shir, A et al. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. EGF receptor-targeted synthetic double-stranded RNA eliminates glioblastoma, breast cancer, and adenocarcinoma tumors in mice. PLoS Med. Volume 3, Issue 1, Pages e6, Jan 2006
    Species: Human
    Sample type: Conditioned Media (poly IC-transfected cells)
  10. Korkaya H, Kim G, Davis A, et al. Activation of an IL-6 Inflammatory Loop Mediates Trastuzumab Resistance in HER2 Overexpressing Breast Cancers by Expanding the Cancer Stem Cell Population. Molecular cell 2012;47(4):570-584. doi:10.1016/j.molcel.2012.06.014.
    Species: Human
    Sample type: Conditioned Media (MCF-7 breast carcinoma cell lines)
The protocol is easy to follow. This kit only needs a small sample volume and can detect a low concentration of cytokine signaling.

University of Kansas
The Human Cytokine Array C5 kit gave me satisfied candidates under the conditioned medium. I strongly recommend this!!

Winship Cancer Institute of Emory University
easy to use and analysis, got excellent results!

NC State University
Simple to use and detects many cytokines

Johns Hopkins University School of Medicine
It works great using the cell growth media.

Emory University
I used on mammalian cell culture supernatant it and works well

Indiana University
Kit worked well with conditioned media from smooth muscle cells. Provides a lot of data in a small amount of time.

University of Nevada School of Medicine
I wanted to get something that can work on primary cell line and it turned out good. Results received after experiments had let us plan on which cytokine study we might need to work on for further study. Kit is simple and was of good quality.

University of Texas Health Science Center San Antonio
Easy to use, works well.

East Tennessee State University
Easy to use, and protocols are easy to follow. Recommended to fellow colleagues.

UCSF
Protocol is simple and worked great with our conditioned media!

UNMC
Simple and easy protocol with clean results.

Augusta University
The protocol suggested in the kit works very well for all arrays. Even if you need to optimize the time using the short exposition time suggested in the protocol, your array development will work great as usual!

Augusta University
Worked great with conditioned media. Followed the protocol in the kit and the results were super clean and of excellent quality.

Stanford University
it's an easy kit to use. However, I was not very impressed with their excel software. there was a mistake which we caught and ask them to fix bit they didn't even give us a discount. Other than it's straightforward and give you great results.

University of the pacific
Worked great for med-high abundant proteins. Customer service was spectacular

UCLA
This kit can detect huge amount of different cytokines and the price is only a little bit more than the kits that only test around 10 cytokines.

Nanjing Medical University
works good with condition media
Human Cytokine Array C5 kit is simple to use, has high quality and results are reproducible. Fast delivery from Raybiotech. But the value for the money is a little high.
Write A Review
U.S. Non-U.S.

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

Risk-Free Guarantee

Amazon Gift Card Program

C-Series Testing Service C-Series Testing Service