Introduction
The RayBio® DNAse Activity Assay offers a quick, sensitive, and straightforward approach to measuring total nuclease activity using a compatible Real-Time PCR instrument. This kit tracks the increase in FAM fluorescence produced from the degradation of the FAM-labeled double-stranded DNA (dsDNA) hydrolysis probe (as shown in Figure 1) by nucleases in the sample. The assay is capable of detecting nuclease activity ≤ 1.03 x 10-4 Units .
Product Features
- Quick and easy nuclease activity measurement
- Detects nuclease activity via fluorescence increase
- High Sensitivity
Kit Components
- dsDNA Probe
- Nuclease Standard
- Cleavage Buffer
- Sample Diluent
- Assay Diluent
Additional Materials Required
- Vortex Mixer
- Tabletop centrifuge
- Pipettes and nuclease-free pipette tips (barrier tips recommended)
- Sterile RNase/DNAse free cotton swabs (if testing environmental swabs)
- Nuclease free dH2O
- Plate reader or PCR thermocycler
- Nuclease free 96-Well ELISA or PCR plate
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed.
- Add Master Mix to each well and mix.
- Add standard or sample to each well of the microplate.
- Measure fluorescence at 494nm excitation and 518nm emission.
Storage/Stability
Store the kit components according to storage temperatures for up to 3 months. Protect dsDNA Probe from light. Kit must be used within expiry period to ensure optimal performance. Do not mix reagents from different kit lots.
