Antibody arrays allow researchers to conduct rapid, accurate expression profiling of hundreds of cytokines, chemokines, growth factors, proteases, soluble receptors and other proteins from any biological fluid.
RayBio® Glass Slide-based Antibody Arrays (G-Series) were introduced as a “second generation” array platform after the chemiluminescent membrane arrays (C-Series). The G-Series uses a 75mm x 25mm glass chip platform which delivers easier handling along with the use of much lower sample volumes. The glass slides also utilize a fluorescent signal readout, allowing a wider dynamic detection range than can be achieved by chemiluminescence.
G-Series Antibody Arrays are particularly well-suited for projects with very limited sample volumes (as little as 10 μl per array) and large numbers of samples. Dozens of arrays can be processed per day by a single technician. Furthermore, the slide chamber assembly is compatible with many automated array processing systems, allowing high-throughput studies to be performed with very little hands-on time.
For a comparison & overview of all our antibody array systems, click here.
Large dynamic range of detection (4 orders of magnitude)
Compatible with many sample types
Low sample consumption: as little as 10 µl of original sample required per array
Customizable – create a custom array from our list of targets
High-throughput profiling of cytokine expression
Identifying potential molecular targets for drug development
Identifying the molecular mechanisms of drug action<
Identifying crucial factors involved in disease processes
Discovering biomarkers for disease management
Discovering expression patterns for molecular classification of diseases
How It Works
Similar to the C-Series (membrane-based) arrays, G-Series Antibody Arrays utilize the sandwich-ELISA design principle. In this assay, capture antibodies are printed in multiple identical arrays on a standard-sized histology slide. After a blocking step, samples are incubated with the arrays. Nonspecific proteins are then washed off, and the arrays are incubated with a cocktail of biotinylated detection antibodies, followed by a streptavidin-conjugated fluor. Signals are visualized using a fluorescence laser scanner.
For a list of laser scanner specifications, click here.
If you do not have access to a scanner, RayBiotech now offers FREE scanning and data extraction service for all G-Series Arrays. Click to learn more
Contents of kit
RayBio® Cytokine Antibody Array glass slide (4 or 8 arrays per slide)
Biotinylated Detection Antibodies
Streptavidin-conjugated HiLytePlus™ 555 Fluor
20X Wash Buffer I
20X Wash Buffer II
2X Cell Lysis Buffer
G-Series Antibody Array accessories*
*Accessories include: 16-well incubation chamber with gasket, protective cover, snap-on sides, adhesive film
Comparison to ELISA
More Data, Less Sample: G-Series Antibody arrays provide high-content screening using less sample volume than ELISA. Only 50 µl needed (after dilution) to detect up to 40 different biomarker proteins. As little as 10 µl original sample!
Global View of Cytokine Expression: Antibody array screening improves the chances for discovering key factors, disease mechanisms or biomarkers related to cytokine signaling.
Greater Sensitivity: As little as 4 pg/ml of MCP-1 can be detected using the G-Series array format. In contrast, our similar MCP-1 ELISA assay has a sensitivity of 40 pg/ml of MCP-1.
Increased Range of Detection: ELISA assays typically detect a concentration range of 100- to 1000-fold, however, RayBiotech arrays can detect IL-2 at concentrations of 25 to 250,000 pg/ml, a range of 10,000-fold.
Better Precision: As determined by densitometry, the inter-array Coefficient of Variation (CV) of spot signal intensities is 5-10%, comparing favorably with ELISA testing (CV = 10-15%).
Qian D., Lu Z., Xu Q., et al. Galectin-1-driven upregulation of SDF-1 in pancreatic stellate cells promotes pancreatic cancer metastasis. Cancer Lett. 2017 Mar 21;397:43-51. doi: 10.1016/j.canlet.2017.03.024. [view publication] Product: Human Chemokine Array G1 Sample Type: Conditioned Media
Wang, N., Li, Q., Zhang, L., Lin, H., Hu, J., Li, D., ... & Wan, J. (2012). Mesenchymal stem cells attenuate peritoneal injury through secretion of TSG-6. PloS one, 7(8), e43768. [view publication] Product: Rat Cytokine Array G2 Sample Type: Conditioned Media
Asif M., Al-Masoub M., Sultan Kahn M., et al. Molecular mechanisms responsible for programmed cell death-inducing attributes of terpenes from Mesua ferrea stem bark towards human colorectal carcinoma HCT 116 cells. Journal of Applied Biomedicine, Oct 18 2016, epub ahead of print [view publication] Product: Human Apoptosis Array G1 Sample Type: Cell Lysate
Asif M., Shafaei A., Jafari S., et al. Isoledene from Mesua ferrea oleo-gum resin induces apoptosis in HCT116 cells through ROS-mediated modulation of multiple proteins in the apoptotic pathways: A mechanistic study. Toxicol Lett. 2016 Aug 22;257:84-96. doi: 10.1016/j.toxlet.2016.05.027. [view publication] Product: Human Apoptosis Array G1 Sample Type: Cell Lysate
Chen, Yao, et al. "WAP four-disulfide core domain protein 2 gene (WFDC2) is a target of estrogen in ovarian cancer cells." Journal of ovarian research 9.1 (2016): 10. [view publication] Product: Human Apoptosis Array G1 Sample Type: Cell Lysate
Gao Y., Zhou Z., Lu S., et al. Chemokine CCL15 Mediates Migration of Human Bone Marrow-Derived Mesenchymal Stem Cells Toward Hepatocellular Carcinoma. Stem Cells. 2016 Jan 13. doi: 10.1002/stem.2275. [view publication] Product: Human Cytokine Array G3 Sample Type: Conditioned Media
Qin Y., Zhang S., Den S., et al. Epigenetic silencing of miR-137 induces drug resistance and chromosomal instability by targeting AURKA in multiple myeloma. Leukemia. 2017 Jan 6. doi: 10.1038/leu.2016.325. [view publication] Product: Human Apoptosis Array G1 Sample Type: Cell Lysate
Vujosevic S., Micera A., Bini S., et al. Proteome analysis of retinal glia cells-related inflammatory cytokines in the aqueous humour of diabetic patients. Acta Ophthalmol. 2016 Feb;94(1):56-64. doi: 10.1111/aos.12812 [view publication] Product: Human Inflammation Array G3 Sample Type: Aqueous Humor
Wu T., Chang S., Li C., et al. Severe Hepatitis Promotes Hepatocellular Carcinoma Recurrence via NF-κB Pathway-Mediated Epithelial-Mesenchymal Transition after Resection. Clin Cancer Res. 2016 Apr 1;22(7):1800-12. doi: 10.1158/1078-0432.CCR-15-0780. [view publication] Product: Human Inflammation Array G3 Sample Type: Tissue lysate
More references are listed per product on the individual product pages.