Membrane Antibody Arrays: C-Series™
Simultaneously screen and compare expression levels of multiple cytokines, growth factors, proteases, soluble receptors, and other proteins from a wide variety of sample types with a membrane-based RayBiotech C-Series array.
Why C-Series?
Because C-Series arrays enable semi-quantitative measurement of multiple proteins at once, you can move your studies forward quickly and with the confidence of a single study instead of spending extra time, samples, and effort—and introducing additional variability—when you normalize and compare multiple experiments.
In addition, you can rely on the high quality and consistency of every C-Series array thanks to the stringent manufacturing and quality control processes we implement in our US-based manufacturing facilities.
What are C-Series arrays?
Each C-Series array consists of antibodies to cytokines, growth factors, proteases, soluble receptors, and other proteins immobilized onto a nitrocellulose membrane. The array is a semi-quantitative, sandwich-based assay that can report on between 10 and 274 different proteins and uses the same familiar workflow as a typical chemiluminescent Western blot.
What’s in each C-Series kit?
Each C-Series Antibody Array kit includes the key reagents for your assay:
- RayBio® C-Series Antibody Array Membranes
- Biotinylated Detection Antibody Cocktail
- Blocking Buffer
- Wash Buffers 1 and 2
- Cell & Tissue Lysis Buffer
- Detection Buffers C and D
- Plastic Incubation Tray
- Protease Inhibitor Cocktail (in select kits)
- User manual
How It Works
C-Series membrane arrays use a sandwich immunoassay design and the same workflows as a typical Western blot.
- Each array includes capture antibodies immobilized onto a nitrocellulose membrane.
- Incubate your sample with the array to enable binding of target proteins to the corresponding antibody.
- Detect bound protein by adding a cocktail of biotinylated antibodies.
- Visualize with labeled streptavidin using chemiluminescence.
You can use the same chemiluminescence detection systems developed for western blot visualization to measure the chemiluminescence of your C-Series membrane arrays. The entire procedure can be completed in 1 day and is simple enough that a novice researcher can successfully collect data with very few pitfalls and little or no optimization.
How to Handle C-Series Membrane Antibody Arrays
Need a deeper understanding of your array data?
Whether you’re looking for statistically significant differences between groups or wish to conduct a detailed protein-protein interaction (PPI) analysis, our in-house biostatistics and bioinformatics experts can build a tailored computational solution for your antibody and protein array data.
