Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) is a glycolytic enzyme central to energy production that also functions in apoptosis, transcriptional activation, and homeostasis, and acts as a sensor of oxidative stress. GAPDH is frequently overexpressed in cancers and under-abundant in neurological diseases such as Alzheimer's and Parkinson's, making it an informative indicator of cellular health, cancer, and neurodegeneration.

Based on a coupled enzyme reaction, glyceraldehyde-3-phosphate is catalyzed by GAPDH to a colored product quantified at 450 nm. The change in absorbance is directly proportional to GAPDH activity in the sample.

NADH standard: 0–250 µM.

Store the entire kit below -20°C for up to 6 months from the date of shipment. After preparation, store individual reagents as described in the kit manual and avoid repeated freeze-thaw cycles.