| Size | 100 tests |
| Estimated Lead Time | 3-5 business days |
| Sensitivity | 2.72 pg/mL |
| Specificity | Contaminating gDNA from E. coli and CHO was tested at 10 ng and 1 ng per reaction. E. coli gDNA had no effect at either concentration. |
| Compatible Sample Types | Extracted DNA, Extracted RNA |
| Quantitative/Semi-Quantitative | Quantitative |
| Method Of Detection | PCR |
| Research Area | Bioprocesses |
| Shipping Type | Dry ice (additional shipping fee) |
| Storage | -20°C |
RayBio® HEK293 Residual DNA Quantification Kit is a ready to use real-time PCR assay for the rapid and reliable detection of HEK293 genomic DNA in biomanufacturing products. This assay is highly sensitive (≤ 5 pg/mL) and resistant to DNA fragmentation. An internal positive control, RNase P, is also detected in each reaction to monitor impact of potential PCR inhibitors.
| Component | Size / Qty |
|---|---|
| PCR Master Mix | 1 tube |
| Primer and Probe Mix | 1 tube |
| HEK293 DNA Control | 1 tube |
| Dilution Buffer | 2 tubes |
Contact our technical support team for questions about compatibility: techsupport@raybiotech.com
The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C (not exceeding 12 months since date of manufacture).
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Assay sensitivity and precision were assessed across four replicates ran over multiple days.
Assay sensitivity and precision were assessed across four replicates ran over multiple days.