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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Sensitivity | 0.16 ng/mL |
| Detection Range | 0.16 ng/mL - 25 ng/mL |
| Specificity | This ELISA antibody pair detects human APRIL. Other species not determined. |
| Compatible Sample Types | Plasma, Serum, Cell Culture Media |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | O75888 |
| Gene Id | 8741 |
| Gene Symbols | TNFSF13, APRIL, TALL2, ZTNF2 |
| Protein Name / Synonyms | Tumor necrosis factor ligand superfamily member 13; A proliferation-inducing ligand (APRIL); TNF- and APOL-related leukocyte expressed ligand 2 (TALL-2); TNF-related death ligand 1 (TRDL-1) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Cancer, Cell Cycle, Autoimmune Disease |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
| Sample Type | Average % Recovery | Range (%) |
|---|---|---|
| Serum | 115.3 | 100-134 |
| Plasma | 106.5 | 70-135 |
| Cell culture media | 126.1 | 104-138 |
Linearity Results
| Sample Type | Serum | Plasma | Cell Culture Media | |
|---|---|---|---|---|
| 1:2 | Average % of Expected | 115.6 | 100.9 | 99.61 |
| Range (%) | 106-125 | 87-118 | 85-114 | |
| 1:4 | Average % of Expected | 110.5 | 116.8 | 106.3 |
| Range (%) | 106-115 | 100-132 | 102-111 | |
Kit Components
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
- Adhesive Sealing Film
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 µL volumes
- Adjustable 1-25 µL pipettes for reagent preparation
- 100 µL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard or sample dilutions
- Plastic wrap
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 µL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Human APRIL ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified APRIL (TNFSF13), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.