Human Autoimmune Disease IgG Autoantibody Array G1

$559.79

RayBio® Autoimmune Disease (AID) IgG Autoantibody Array G1 contains 33 human proteins and double strand DNA(dsDNA) for simultaneous detection of human IgG autoantibodies presented in blood serum samples of common 10 autoimmune diseases, such as systemic lupus erythematosus (SLE), Sjøgren's syndrome, Rheumatoid arthritis, primary biliary cirrhosis, etc.

PAH-AIDG-G1-16
+

Antigen Information

Gene Symbol:
CALCA | CENPA | CENPB | GARS | HARS | KRT19 | KRT20 | KRT8 | MPO | NUP210   View more

Assay Format

Number of Targets Detected:
33
Species Detected:
  • Human
Compatible Sample Types:
  • Serum
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
16 Sample Kit

Product Features

  • Small sample volume required (2 µl)
  • High density (simultaneously detect 33 autoantibodies)
  • High sensitivity
  • Large dynamic range
  • Suitable for high-throughput assays
  • High efficiency and accuracy
  • Affordable, quick and simple to use
  • Customizable: Tell us the autoimmunogens you're interested in

Target Name

Scroll over each target protein for more information

Alanyl-tRNA Synthetase
Cytokeratin 8
(KRT8)
Centromere protein B
(CENP-B)
  • Gene Symbol: CENPB
  • Gene ID: 1852
  • Accession #: P07199
Centromere protein A
(CENP-A)
  • Gene Symbol: CENPA
  • Gene ID: 1851
  • Accession #: P49450
dsDNA
Gliadin
Glycine-tRNA ligase
(AP-4-A synthetase/GlyRS)
  • Gene Symbol: GARS
  • Gene ID: 4162
  • Accession #: P41250
Nuclear pore protein gp210
(POM210/Nucleoporin Nup210)
  • Gene Symbol: NUP210 KIAA0906 PSEC0245
  • Gene ID: 30052
  • Accession #: Q8TEM1
Histone H2A
Histone H2B
Cytokeratin 19
(KRT19)
Histidine-tRNA ligase
(HisRS)
  • Gene Symbol: HARS
  • Gene ID: 4816
  • Accession #: P12081
Lupus La protein
(SS-B)
  • Gene Symbol: SSB
  • Gene ID: 11316
  • Accession #: P05455
LC-1
Cytokeratin 20
(KRT20)
M/Scl 100
M2
Mi-2
Myeloperoxidase
  • Gene Symbol: MPO
  • Gene ID: 4353
  • Accession #: P05164
Nuclear pore glycoprotein p62
(Nucleoporin Nup62)
  • Gene Symbol: NUP62
  • Gene ID: 8066
  • Accession #: P37198
Calcitonin
PCNA
Myeloblastin
(PR3/C-ANCA)
  • Gene Symbol: PRTN3 MBN
  • Gene ID: 9495
  • Accession #: P24158
TRIM21
(Ro(SS-A)/SS-A)
  • Gene Symbol: TRIM21 RNF81 RO52 SSA1
  • Gene ID: 11312
  • Accession #: P19474
TRIM21
(Ro(SS-A)/SS-A)
  • Gene Symbol: TRIM21 RNF81 RO52 SSA1
  • Gene ID: 11312
  • Accession #: P19474
DNA topoisomerase 1
(DNA topoisomerase I)
  • Gene Symbol: TOP1
  • Gene ID: 11986
  • Accession #: P11387
Sm core protein
Sp-100
  • Gene Symbol: SP100
  • Gene ID: 11206
  • Accession #: P23497
Threonine-tRNA ligase
(ThrRS)
  • Gene Symbol: TARS2
  • Gene ID: 30740
  • Accession #: Q9BW92
Thyroglobulin
Thrombopoietin
(TPO)
Transglutaminase 2
(TGM2)
snRNP70
  • Gene Symbol: SNRNP70 RNPU1Z RPU1 SNRP70 U1AP1
  • Gene ID: 11150
  • Accession #: P08621
  

Application Notes

Suggested Application

IgG autoantibody profiling for following common autoimmune diseases, but may not be limited to:

  1. Systemic lupus erythematosus (SLE)
  2. Sjøgren's syndrome
  3. Rheumatoid arthritis
  4. Systemic Sclerosis/CREST syndrome
  5. Primary biliary cirrhosis
  6. Autoimmune hepatitis
  7. Hashimoto's thyroiditis
  8. Polymyositis/Dermatomyositis
  9. System vasculitis
  10. Celiac disease

The following table shows multiple IgG-type autoantibodies that can be detected in patient sera of different autoimmune diseases.

Autoimmune DiseasesTarget No.Autoimmunogens
Systemic lupus erythematosus (SLE)#5dsDNA
#9Histone H2A
#10Histone H2B
#22Proliferating Cell Nuclear Antigen (PCNA)
#26Scl-70 (DNA topoisomerase-1)
#33U1-snRNP70 kDa
#12Jo-1 (histidyl tRNA synthetase, histidine-tRNA ligase)
#27Sm/Smith/snRNP core protein
Rheumatoid arthritis#21CCP peptide
#2KRT8
#11KRT19
#15KRT20
Systemic Sclerosis / CREST Syndrome#4Centromere Protein A (CENP-A)
#3Centromere B (CENP-B)
#16M/Scl 100
#27Sm/Smith/snRNP core protein
Primary biliary cirrhosis#8gp-210
#17M2
#20Nup62
#28sp100
Autoimmune hepatitis#14Liver cystolic antigen type 1 (LC-1)
Hashimoto's thyroiditis#30Thyroglobulin (TG)
#31Thyroid peroxidase (TPO)
Polymyositis / Dermatomyositis#1Alanyl-tRNA Synthetase (PL-12)
#7Glycyl-tRNA Synthetase (EJ)
#18Mi-2
#29Threonyl-tRNA synthetase (PL-7)
#14Liver cystolic antigen type 1 (LC-1)
System vasculitis#19Myeloperoxidase (MPO), p-ANCA
#23Proteinase-3 (PR3), c-ANCA
#3Centromere B (CENP-B)
Sjøgren's syndrome#13La/SS-B
#24Ro/SS-A (52 kDa)
#25Ro/SS-A (60 kDa)
Celiac disease#6Gliadin
#32Tissue Transglutaminase (tTG)
Kit Components
  • RayBio® Human Autoimmune Disease IgG Autoantibody G1 array slide
  • RayBio® G-series antibody array accessory (including slide incubation chamber assembly, gasket, protective cover, snap-on sides and adhesive film)
  • Blocking Buffer (8 ml/bottle)
  • Wash Buffer 1 (30 ml/bottle)
  • Wash Buffer 2 (30 ml/bottle)
  • 1,000X Biotin-conjugated anti-human IgG (1.5 µl/1 vial)
  • 1,500X HiLyte 555 Streptavidin (1 µl/vial)
Other Materials Required
  • Small plastic boxes or containers
  • Orbital shaker or oscillating rocker
  • Pipettors, pipette tips and other common lab consumables
  • Distilled water
  • Aluminum foil
  • Laser scanner for fluorescence detection
Protocol Outline

See Manual for relevant protocols.

Below is the quick protocol for the experienced users.

The quick protocol for the experienced users.

Typical Data

The following Figure 1 represents a typical result using serum on the RayBio® Autoimmune Disease IgG Autoantibody Detection Array (Cat #. PAH-AIDG-G1). The protein arrays were incubated with 200-fold diluted serum samples from normal individuals and patients with different autoimmune diseases. After washing steps, the arrays were sequentially incubated with biotin-labeled anti-human IgG antibody and fluorescent dye-labeled streptavidin. The signals from the bound autoantibodies were captured with a fluorescence scanner.

Sample diluent alone (Figs. 1A & 1B) and normal sera (Figs. 1C & 1D) were used as negative controls, and the arrays signals indicate that no significant background occurred. In some cases, a negligible signal can be observed, which may be caused by non-specific binding derived from the biotin-labeled anti-human IgG antibody and/or fluorescent dye-labeled streptavidin. However, these weak background signals will be subtracted in subsequent data analysis procedures and will not affect sample test results. A strong binding signal was detected in multiple targets among different autoimmune disease patients: systemic lupus erythematosus (Figs.1E & 1F), Sjøgren's syndrome (Figs.1G & 1H), system vasculitis (Figs.1I & 1J), and primary biliary cirrhosis (Figs.1K & 1L).

Typical result using serum on the RayBio<sup>®</sup> Autoimmune Disease IgG Autoantibody Detection Array.

Fig 1. Typical result using serum on the RayBio® Autoimmune Disease IgG Autoantibody Detection Array. The protein arrays were incubated with diluent alone (A, B), diluted serum samples derived from normal individuals (C, D) and those from various autoimmune disease patients (E-L). Yellow boxes, show various controls as indicated (biotin-labeled BSA, human IgG and IgA).

Storage/Stability

Upon receipt, all components of the RayBio® Human Protein Array kit should be stored at -20 °C to -80 °C until just before the experiment. At -20 °C to -80 °C the kit will retain complete activity for up to 6 months. Please use within six months of purchase. Once thawed, the array glass slide and Blocking Buffer should be kept at -20 °C and all other component should be stored at 4 °C. Use within three months after reagents have been thawed.

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Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Total list price $675.94
Price for all $594.63

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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