Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
14-3-3 beta, 14-3-3 epsilon, 14-3-3 eta, 14-3-3 gamma, 14-3-3 sigma, 14-3-3 theta, 14-3-3 zeta, ABL1, ANAPC1, ANAPC13, ANAPC2, ANAPC4, ATM, ATR, BUB1, BUB3, CBP, CCNA1, CCNA2, CCNB2, CCND2, CCND3, CCNE1, CCNE2, CDC14A, CDC14B, CDC16, CDC2, CDC23, CDC25A, CDC25B, CDC25C, CDC26, CDC27, CDC6, CDK2, CDK4, CDK6, CDK7, CDKN1C, CDKN2A, CDKN2B, CDKN2C, CDKN2D, CHEK1, CHEK2, Cyclin D1, E2F1, E2F2, E2F4, E2F5, EP300, GADD45A, GADD45B, GADD45G, GSK3B, HDAC1, HDAC2, MAD1L1, MAD2L1, MCM5, MCM7, MCMP2, MDM2, MYC, ORC3L, p21, p27/Kip1, p53, PCNA, PLK1, PRKDC, PTTG2, RB1, RBL1, RBL2, SKP2, Smad 4, SMAD2, SMAD3, SMC1A, STAG1, TFDP1, TFDP2, TGF beta 1, TGF beta 2, TGF beta 3, TTK, ZBTB17
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Glass Slide(s)
- Spin Columns
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Streptavidin-Conjugated Fluor
- Adhesive Plastic Strips
- Labeling Buffer
- Lysis Buffer
- 30 ml Centrifuge Tube
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- 1 ml tube
- Pipettors, pipette tips and other common lab consumables
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Dry the glass slide
- Block array surface
- Incubate with Samples
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.