Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
Adipsin, Alpha-2 Macroglobulin, BDKRB1, BDKRB2, C1QA, C1QB, C1QC, C1R, C1S, C2, C3a, C3AR1, C4A, C4BPA, C4C, C5a, C5AR1, C5b-9, C6, C7, C8B, C8G, C9, CD21, CD35, CD46, CD55, CD59, CFB, CFH, CFHR1, CFHR2, CFHR4, CFI, CFVII, Clusterin, Coagulation Factor XIV, Complement Factor H-related 5, CPB2, EPCR, F11, F2RL3, F3, F8, Factor IX, Factor V, Factor XII, Factor XIII, Factor XIII B, FACX, FGG, Fibrinopeptide A, Fibrinopeptide B, ITGAX, ITGB2, Kininogen, KLK-B1, MAC-1, MASP2, MASP3, MBL2, PAI-1, PAR1, Plasminogen, PROS1, Serpin A1, Serpin A5, Serpin B2, Serpin C1, Serpin D1, Serpin F2, Serpin G1, TFPI, Thrombin, Thrombomodulin, TPA, uPA, uPAR, Vitronectin, VSIG4, VWF
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Glass Slide(s)
- Spin Columns
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Streptavidin-Conjugated Fluor
- Adhesive Plastic Strips
- Labeling Buffer
- Lysis Buffer
- 30 ml Centrifuge Tube
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- 1 ml tube
- Pipettors, pipette tips and other common lab consumables
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Dry the glass slide
- Block array surface
- Incubate with Samples
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) at -20°C and all other reagents undiluted at 4°C for no more than 3 months.