Human Cytokine Array G1000

RayBio® G-Series Human Cytokine Antibody Array 1000 Kit. A combination of Human Cytokine Antibody Array G6 & G7. Detects 120 Human Cytokines. Suitable for all liquid sample types.

AAH-CYT-G1000-4
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Antigen Information

Gene Symbols:
ADIPOQ | AGRP | ANG | ANGPT2 | AREG | AXL | BDNF | BMP4 | BMP6 | BTC   View more

Assay Format

Number of Targets Detected:
120
Species Detected:
  • Human
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
Semi-Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
4 or 8 Sample Kit

Product Features

  • High sensitivity and specificity
  • Low sample volume (10-100 µl per array)
  • Large dynamic range of detection
  • Compatible with most sample types
  • Test 4 or 8 samples on each slide
  • Suitable for high-throughput assays

Target Names

Scroll over each target protein for more information
Adiponectin
(ACRP30)
AgRP
Amphiregulin
Angiogenin
Angiopoietin-2
Axl
BDNF
bFGF
BLC
(CXCL13)
BMP-4
BMP-6
beta-NGF
Betacellulin
(BTC)
CCL28
(MEC)
CK beta 8-1
(CCL23)
CNTF
CTACK
(CCL27)
Dtk
EGF
EGFR
ENA-78
(CXCL5)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Eotaxin-3
(CCL26)
Fas
(TNFRSF6/Apo-1)
FGF-4
FGF-6
FGF-7
(KGF)
FGF-9
Flt-3 Ligand
Fractalkine
(CX3CL1)
GCP-2
(CXCL6)
GCSF
GDNF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
GRO alpha/beta/gamma
GRO alpha
(CXCL1)
HCC-4
(CCL16)
HGF
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
ICAM-3
(CD50)
IFN-gamma
IGFBP-1
IGFBP-2
IGFBP-3
IGFBP-4
IGFBP-6
IGF-1
IGF-1 R
IL-1 R4
(ST2)
IL-1 R1
IL-10
IL-11
IL-12 p40
IL-12 p70
IL-13
IL-15
IL-16
IL-17A
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 ra
(IL-1 F3)
IL-2
IL-2 R alpha
IL-3
IL-4
IL-5
IL-6
IL-6 R
IL-7
IL-8
(CXCL8)
I-TAC
(CXCL11)
Leptin
Light
(TNFSF14)
Lymphotactin
(XCL1)
MCP-1
(CCL2)
MCP-2
(CCL8)
MCP-3
(MARC/CCL7)
MCP-4
(CCL13)
M-CSF
MDC
(CCL22)
MIF
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 delta
(CCL15)
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MSP alpha/beta
NAP-2
(PPBP/CXCL7)
NT-3
NT-4
Oncostatin M
Osteoprotegerin
(TNFRSF11B)
PARC
(CCL18)
PDGF-BB
PLGF
RANTES
(CCL5)
SCF
SDF-1 alpha
(CXCL12 alpha)
gp130
TNF RII
(TNFRSF1B)
TNF RI
(TNFRSF1A)
TARC
(CCL17)
TECK
(CCL25)
TGF beta 3
  • Gene Symbol: TGFB3
  • Gene ID: 7043
  • Accession #: P10600
TGF beta 1
Thrombopoietin
(TPO)
TIMP-1
TIMP-2
TNF alpha
TNF beta
(TNFSF1B)
TRAIL R3
(TNFRSF10C)
TRAIL R4
(TNFRSF10D)
uPAR
VEGF-A
VEGF-D

Species Detected

Human

Application Notes

Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Detecting Nephrotoxicity
Kit Components
  • Human Cytokine Antibody Array G1000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Lysis Buffer
  • Adhesive Plastic Strips
  • 30 ml Centrifuge Tube
  • Manual
Other Materials Required
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Block array surface
  3. Incubate with Sample
  4. Incubate with Biotinylated Detection Antibody Cocktail
  5. Incubate with Streptavidin-Conjugated Fluor
  6. Disassemble the glass slide
  7. Scan with a gene microarray laser scanner
  8. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Pasquier J., Gosset M., Geyl C., et al. CCL2/CCL5 secreted by the stroma induce IL-6/PYK2 dependent chemoresistance in ovarian cancer. https://www.ncbi.nlm.nih.gov/pubmed/29455640
    Species: Human
    Sample type: Conditioned Media
  2. Kastallari E., Baba-Amer Y., Alonso-Martin S., et al. Pericytes in the myovascular niche promote post-natal myofiber growth and satellite cell quiescence. Development. 2015 Apr 1;142(7):1242-53. doi: 10.1242/dev.115386.
    Species: Human
    Sample type: Conditioned Media
  3. Gomez DL, O’Driscoll M, Sheets TP, Hruban RH, Oberholzer J, McGarrigle JJ, et al. (2015) Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate. PLoS ONE 10(8): e0133862. doi:10.1371/journal.pone.0133862
    Species: Human
    Sample type: Conditioned Media
  4. Matte I., Lane D., Laplante C., et al. Ovarian cancer ascites enhance the migration of patient-derived peritoneal mesothelial cells via cMet pathway through HGF-dependent and -independent mechanisms. Int J Cancer. 2014 Dec 6. doi: 10.1002/ijc.29385.
    Species: Human
    Sample type: Peritoneal Fluid
  5. Matte, I., Land D., Bachvarov D., Rancourt C., Piche A. Role of malignant ascites on human mesothelial cells and their gene expression profiles. BMC Cancer 2014,14:288 doi:10.1186/1471-2407-14-288
    Species: Human
    Sample type: Conditioned Media
  6. Agarwal A., Cooke L., Riley C., Qi W., Mount D., et al. Genetic and cytokine changes associated with symptomatic stages of CLL. Leukemia Research Available online 18 June 2014 In Press, Accepted Manuscript
    Species: Human
    Sample type: Serum
  7. Fan, Xiubo, et al. "Mesenchymal stromal cell supported umbilical cord blood ex vivo expansion enhances regulatory T cells and reduces graft versus host disease." Cytotherapy 15.5 (2013): 610-619.
    Species: Human
    Sample type: Conditioned Media
  8. Laberge R-M, Zhou L, Sarantos MR, et al. Glucocorticoids Suppress Selected Components of the Senescence-Associated Secretory Phenotype. Aging Cell 2012;11(4):569-578. doi:10.1111/j.1474-9726.2012.00818.x.
    Species: Human
    Sample type: Conditioned Media
  9. Straussman R., Morikawa T., et al. Tumourmicro-environment elicits innate resistance to RAF inhibitors through HGF secretion. Nature. 2012 Jul 26;487(7408):500-4. doi: 10.1038/nature11183.
    Species: Human
    Sample type: Conditioned Media
  10. Matte I, Lane D, Laplante C, Rancourt C, Piché A. Profiling of cytokines in human epithelial ovarian cancer ascites. American Journal of Cancer Research. 2012;2(5):566-580.
    Species: Human
    Sample type: Peritoneal Fluid

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Analysis Tool Bundle Discount

70% off the data analysis tool when you purchase the array and tool together!


Note: If you are not placing this order online, make sure to mention this bundle discount to our orders department (orders@raybiotech.com).

Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.