Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

RayBio^® Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit. This assay semi-quantitatively measures PDK1 phosphorylated at Serine-241 as well as total PDK1 in adherent cell lines.

$397.00

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human, Mouse, Rat
Specificity	This ELISA kit recognizes Human PDK1 phosphorylated at site Serine-241 as well as total PDK1.
Compatible Sample Types	Adherent Cell Culture
Accession Number	O15530
Gene Id	5170
Gene Symbols	PDK1, PDPK1
Protein Name / Synonyms	3-phosphoinositide-dependent protein kinase 1 (hPDK1) (EC 2.7.11.1)
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Cell-based
Method Of Detection	Colorimetric
Research Area	Post-Translational Modifications, Phosphorylation
Shipping Type	Blue ice
Storage	-20°C

Product Features

Rapidly measure phosphorylated protein in adherent cell lines
Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
No sample lysis is needed
Compatible with a standard ELISA plate reader

Kit Components

Uncoated 96-Well Microplate
Wash Buffers
Fixing Solution
Quenching Buffer
Blocking Buffer
Anti-phospho Protein Antibody
Anti-pan Protein Antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

A model cell line, protein tyrosine kinase inhibitors, growth factors or cytokines
Microplate reader capable of measuring absorbance at 450 nm
37°C incubator
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Orbital shaker or oscillating rocker

Assay Procedure Summary

Seed 30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 minutes at room temperature.
Add 200 µl of prepared Quenching Buffer and incubate 20 minutes at room temperature.
Add 200 µl of prepared Blocking Buffer and incubate for 1 hour at 37°C.
Add 50 µl of prepared primary antibody to each well and incubate for 2 hours at room temperature.
Add 50 µl of prepared HRP-Conjugated Secondary Antibody and incubate for 1 hour at room temperature.
Add 100 µl of TMB One-Step Substrate Reagent and incubate 30 minutes at room temperature.
Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

Store entire kit at ≤ -20 °C immediately upon arrival. Kit must be used within the 6 month expiration date. Avoid repeated freeze-thaw cycles.

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Get your lot-specific datasheet at Ray's Docs. The datasheet linked below is our current version and may not match your lot.

Datasheet (CBEL-PDK1-S241.pdf)

SDS (b03d400912f456f237e869a7ec226e1ce554fb1d.pdf)

Product Features

Rapidly measure phosphorylated protein in adherent cell lines
Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
No sample lysis is needed
Compatible with a standard ELISA plate reader

Kit Components

Uncoated 96-Well Microplate
Wash Buffers
Fixing Solution
Quenching Buffer
Blocking Buffer
Anti-phospho Protein Antibody
Anti-pan Protein Antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate Reagent
Stop Solution

Additional Materials Required

A model cell line, protein tyrosine kinase inhibitors, growth factors or cytokines
Microplate reader capable of measuring absorbance at 450 nm
37°C incubator
Precision pipettes to deliver 2 µL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Absorbent paper
Distilled or deionized water
Orbital shaker or oscillating rocker

Assay Procedure Summary

Seed 30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacturer's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 minutes at room temperature.
Add 200 µl of prepared Quenching Buffer and incubate 20 minutes at room temperature.
Add 200 µl of prepared Blocking Buffer and incubate for 1 hour at 37°C.
Add 50 µl of prepared primary antibody to each well and incubate for 2 hours at room temperature.
Add 50 µl of prepared HRP-Conjugated Secondary Antibody and incubate for 1 hour at room temperature.
Add 100 µl of TMB One-Step Substrate Reagent and incubate 30 minutes at room temperature.
Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.

Storage/Stability

Store entire kit at ≤ -20 °C immediately upon arrival. Kit must be used within the 6 month expiration date. Avoid repeated freeze-thaw cycles.

Featured Products

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Featured Products

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Have this product in hand?

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

Featured Products

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Featured Products

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit

Product Description

Specifications

Product Features

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

Human/Mouse/Rat Phospho-PDK1 (Ser241) and Total PDK1 Cell-Based ELISA Kit