Immuno-PCR Ultrasensitive ELISA - IQELISA™

Less Sample, More Sensitivity

IQELISA SERVICE SALE Full testing service for only $5 More per plate GLP Compliant | Limited time | North America direct customers only

The RayBiotech Immuno-Quantitative ELISA (IQELISA™) is an immuno-PCR assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

How It Works

Immuno-PCR Assay

RayBio® IQELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

IQELISA™ Testing Service

Send Us Your Samples, We'll Send You Results

If you prefer to avoid the learning curve of rtPCR, let us run it for you. Simply mail your samples to RayBiotech, and we will perform full testing service in our GLP-compliant laboratory. To obtain an service quote, fill out the quote request form linked below. Ready to ship your samples? Be sure to fill out the service form linked below.

Full service now only $5 more per plate. Get your quote today!


  • Excellent for follow up/confirmation of antibody array data
  • Pre-coated 96 well plate
  • Complete kit includes all necessary reagents
  • 10x more sensitive than ELISA
  • Reproducible and reliable
  • Optimized for serum, plasma, urine and cell culture medium
  • 1/10th the sample volume of an ELISA

Contents of Kit

  • 96-well pre-coated plate (12x8)
  • Wash Buffer Concentrate (20x)
  • Standards
  • Sample Diluent Buffers
  • Biotin-labeled Detection Reagent
  • Primer Solutions
  • PCR Master Mix
  • PCR Preparation buffer
  • Final Wash Buffer

Representative Data

IQELISA Standard Curve

Research Applications

  • Detection of quantitative protein levels in biological fluids
  • Validation of antibody array results
  • Validation of biomarker discovery studies

Need Even More Sensitivity?

SIMOA® Ultrasensitive Biomarker Testing Service

Single molecule array (Simoa®) testing is a powerful new technique that, like IQELISA™, is orders of magnitude more sensitive than standard sandwich based immunoassay techniques. RayBiotech's Ultrasensitive Simoa Testing Services can achieve sensitivity as low as femtogram (fg/ml) levels, allowing the detection and quantification of biomarkers at concentrations previously difficult or impossible to measure.