The RayBiotech Immuno-Quantitative ELISA (IQELISA™) is an immuno-PCR assay that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
RayBio® IQELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells; the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
If you prefer to avoid the learning curve of rtPCR, let us run it for you. Simply mail your samples to RayBiotech, and we will perform full testing service in our GLP-compliant laboratory. To obtain an service quote, fill out the service request form linked below. Ready to ship your samples? Be sure to fill out the sample service form linked below.
Full service now only $5 more per plate. Get your quote today!
Single molecule array (Simoa®) testing is a powerful new technique that, like IQELISA™, is orders of magnitude more sensitive than standard sandwich based immunoassay techniques. RayBiotech's Ultrasensitive Simoa Testing Services can achieve sensitivity as low as femtogram (fg/ml) levels, allowing the detection and quantification of biomarkers at concentrations previously difficult or impossible to measure.