The mitochondrial genome (mtDNA) is a circular, intron-free, double-stranded, haploid DNA strand 16.5 kb in length that encodes 37 genes. mtDNA copy number (mtDNA-CN) is a biomarker for mitochondrial dysfunction associated with overall mortality and a number of age-related diseases, including cardiovascular disease, chronic kidney disease, and cancer. Mice have become the most ubiquitous model organism used to study human disease. Mice are strikingly similar to humans at the genomic level and the pathophysiology of disease in mice is similar to that of humans. Moreover, mice are a cost-effective and efficient tool to enhance research and drug testing.

The RayBio^® Mouse Absolute Mitochondrial DNA Copy Number Quantification Kit (MTM-CNQ) is a Taqman™ probe-based qPCR assay. Both the primer and probe of mtDNA specifically target one of the most conserved regions on mouse mtDNA. Meanwhile, the primer and probe of the single copy gene (36B4) specifically recognizes and amplifies a 77 bp-long nuclear genomic DNA and serves as a reference for data normalization. The optimized PCR formulation provides superior specificity and efficiency with a wide linear dynamic range. This kit allows for the determination of mouse mitochondrial DNA copy number in one reaction, by the comparison of mitochondrial (mt) and nuclear (n) DNA using various genomic DNA from cultured cells, tissue, saliva, urine, spinal fluid, lavage fluid, blood, etc.

• No off-target amplification on nuclear DNA
• Superior specificity and efficiency
• Wide linear dynamic range
• Measures mtDNA in one reaction
• Compatible with various human samples

• Fluorescence qPCR instrument capable of reading FAM (494nm maximum absorption, 518nm maximum emission) and VIC (532nm maximum absorption, 552nm maximum excitation) channels
• Sterile nuclease-free pipette tips (barrier tips recommended)
• Microfuge tubes
• Compatible PCR Plate
• Microcentrifuge
• Vortex Mixer

Contact our technical support team for questions about compatibility: techsupport@raybiotech.com

1. Prepare all reagents, samples and standards as instructed.
2. Load the PCR reaction mix and samples.
3. Seal the PCR reaction tubes or plate.
4. Set up the PCR Amplification.
5. Export the results.
6. Calculate mtDNA copy number.

The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C. Avoid repeated freeze-thaw cycles.