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Mouse Cytokine Array Q4000

Quantibody® Mouse Cytokine Array 4000 Kit. A combination of Mouse Cytokine Array Q4, Mouse Cytokine Array Q5, Mouse Cytokine Array Q6, Mouse Cytokine Array Q7, and Mouse Cytokine Array Q8. Detects 200 Mouse Cytokines. Suitable for all liquid sample types.

Old price: $4,950.40
$3,960.32  
You save: $990.08 (20%)
QAM-CAA-4000-1
+

Antigen Information

Gene Symbols:
ACE | ADAMTS1 | ADIPOQ | AGER | AHSG | ANGPT4 | ANGPTL1 | ANGPTL3 | ARTN | AXL   View more

Assay Format

Number of Targets Detected:
200
Species Detected:
  • Mouse
Compatible Sample Types:
  • Cell Culture Supernatants
  • Cell Lysates
  • Other Body Fluids
  • Plasma
  • Serum
  • Tissue Lysates
Design Principle:
  • Sandwich-based
Method of Detection:
Fluorescence Laser Scanner
Quantitative/Semi-Quantitative:
  • Quantitative
Solid Support:
Glass Slide

Product Specifications

Size:
1, 2, or 4 Slide Kit (16 sub-arrays per slide)

Product Features

  • More cost-effective than traditional ELISA
  • High specificity and system reproducibility
  • Suitable for diverse sample types
  • Low sample volume requirement: 250 µL or less
  • Get results same day (6-hour processing time)
  • Well-suited for high throughput assays
  • Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
4-1BB
(TNFRSF9/CD137)
6Ckine
(CCL21)
Ace
Activin A
ADAMTS-1
(METH1)
Adiponectin
(ACRP30)
ALK-1
Amphiregulin
ANGPTL1
ANGPTL3
Artemin
Axl
BAFF R
Betacellulin
(BTC)
bFGF
BLC
(CXCL13)
C5a
Cardiotrophin-1
(CT-1)
CCL28
(MEC)
CCL6
CD27
(TNFRSF7)
CD27 Ligand
(TNFSF7)
CD30
(TNFRSF8)
CD30 Ligand
(TNFSF8)
CD32a
CD36
(SR-B3)
CD40
(TNFRSF5)
CD40 Ligand
(TNFSF5)
CD48
(SLAMF2)
CD6
CD80
(B7-1)
Chemerin
Chordin
Clusterin
CRP
(C-Reactive Protein)
CTLA-4
(CD152)
CXCL16
Cystatin C
DAN
Decorin
DKK-1
DLL4
Dtk
E-Cadherin
EDAR
EGF
Endocan
Endoglin
(CD105)
Eotaxin-1
(CCL11)
Eotaxin-2
(MPIF-2/CCL24)
Epigen
Epiregulin
E-Selectin
Fas
(TNFRSF6/Apo-1)
Fas Ligand
(TNFSF6)
Fetuin A
Flt-3 Ligand
Fractalkine
(CX3CL1)
Galectin-1
Galectin-3
Galectin-7
Gas 1
Gas 6
GCSF
GITR
(TNFRSF18)
GITR Ligand
(TNFSF18)
GM-CSF
gp130
Granzyme B
Gremlin-1
H60
HAI-1
HGF
HGFR
I-309
(TCA-3/CCL1)
ICAM-1
(CD54)
IFN-gamma
IFN-gamma R1
IGF-1
IGFBP-2
IGFBP-3
IGFBP-5
IGFBP-6
IL-1 alpha
(IL-1 F1)
IL-1 beta
(IL-1 F2)
IL-1 R4
(ST2)
  • Gene Symbol: IL1RL1
  • Accession #: Q05208
IL-1 ra
(IL-1 F3)
IL-10
IL-12 p40
  • Gene Symbol: IL12B
  • Accession #: P43432
IL-12 p70
  • Gene Symbol: IL12A
  • Accession #: P43431
IL-13
IL-15
IL-17 RB
IL-17A
IL-17B
IL-17E
(IL-25)
IL-17F
IL-2
IL-2 R alpha
IL-20
IL-21
IL-22
IL-23 p19
IL-28A
(IFN-lambda 2)
IL-3
IL-3 R beta
IL-33
(IL-1 F11)
IL-4
IL-5
IL-6
IL-7
IL-7 R alpha
IL-9
I-TAC
(CXCL11)
JAM-A
(CD321/F11R)
KC
(CXCL1)
Kremen-1
Leptin
Leptin R
Limitin
Lipocalin-2
(NGAL)
LIX
LOX-1
L-Selectin
(CD62L)
Lungkine
(CXCL15)
Lymphotactin
(XCL1)
MAdCAM-1
Marapsin
MBL-2
MCP-1
(CCL2)
MCP-5
M-CSF
MDC
(CCL22)
Meteorin
MFG-E8
MIG
(CXCL9)
MIP-1 alpha
(CCL3)
MIP-1 beta
(CCL4)
MIP-1 gamma
MIP-2
MIP-3 alpha
(CCL20)
MIP-3 beta
(CCL19)
MMP-10
MMP-2
MMP-3
Neprilysin
Nope
NOV
(CCN3)
Osteoactivin
(GPNMB)
  • Gene Symbol: GPNMB
  • Accession #: Q99P91
Osteopontin
(SPP1)
Osteoprotegerin
(TNFRSF11B)
OX40 Ligand
  • Gene Symbol: TNFSF4
  • Gene ID: 7292
P-Cadherin
PDGF-AA
Pentraxin-3
(TSG-14)
Periostin
Persephin
Platelet Factor 4
(CXCL4)
PlGF-2
Progranulin
Prolactin
Pro-MMP-9
Prostasin
P-Selectin
RAGE
RANTES
(CCL5)
Renin 1
Resistin
SCF
SDF-1 alpha
(CXCL12 alpha)
sFRP-3
SLAM
(CD150)
Sonic Hedgehog N-Terminal
(Shh-N)
TACI
(TNFRSF13B)
TARC
(CCL17)
TCK-1
(CXCL7)
TECK
(CCL25)
Testican 3
TGF beta 1
  • Gene Symbol: TGFB1
  • Accession #: P04202
Thrombopoietin
(TPO)
TIM-1
(KIM-1)
TNF alpha
TNF RI
(TNFRSF1A)
TNF RII
(TNFRSF1B)
TRAIL
(TNFSF10)
Trance
(TNFSF11)
TREM-1
TREML1
(TLT-1)
TROY
(TNFRSF19)
Tryptase epsilon
TSLP
TWEAK
(TNFSF12)
TWEAK R
(TNFRSF12)
VCAM-1
(CD106)
VEGF-A
VEGF-B
VEGF-D
  • Gene Symbol: FIGF
  • Accession #: A2AIH3
VEGFR1
VEGFR2
VEGFR3

Species Detected

Mouse

Application Notes

Suggested Application
  • Multiplexed Protein Detection
  • Biomarker Screening
  • Identifying Key Factors
  • Confirming Biological Process
  • Biomarker Validation
  • Validation of Antibody Array Results
  • Quantitative Protein Detection
  • Establishing Normal Range
Kit Components
  • Mouse Cytokine Array Q4000 Slide(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Lyophilized Standard Mix
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Slide Washer/Dryer
  • Adhesive Plastic Strips
  • Manual
Other Materials Required
  • Distilled or deionized water
  • Small plastic boxes or containers
  • Pipettors, pipette tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Aluminum foil
  • Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
  1. Dry the glass slide
  2. Prepare Standards
  3. Block array surface
  4. Incubate with Samples and Standards
  5. Incubate with Biotinylated Detection Antibody Cocktail
  6. Incubate with Streptavidin-Conjugated Fluor
  7. Disassemble the glass slide
  8. Scan with a gene microarray laser scanner
  9. Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
  1. Schnepp P., Lee D., Guldner I., et al. GAD1 Upregulation Programs Aggressive Features of Cancer Cell Metabolism in the Brain Metastatic Microenvironment. Cancer Res. 2017 Apr 11. pii: canres.2289.2016. doi: 10.1158/0008-5472.CAN-16-2289.
    Species: Mouse
    Sample type: Conditioned Media (Cancer associated fibroblasts and tumor cells in a transwell setup)
  2. Yang, Y., He, Z., Xing, Z. et al. Influenza vaccination in early Alzheimer’s disease rescues amyloidosis and ameliorates cognitive deficits in APP/PS1 mice by inhibiting regulatory T cells. J Neuroinflammation 17, 65 (2020). https://doi.org/10.1186/s12974-020-01741-4
    Species: Mouse
    Sample type: Serum, Tissue Lysate (brain lysate)
  3. Mao Y., Yen H., Sun Y., Lv Z., Huang R. Development of non-overlapping multiplex antibody arrays for the quantitative measurement of 400 human and 200 mouse proteins in parallel (TECH1P.849). The Journal of ImmunologyMay 1, 2014vol. 192 no. 1 Supplement 69.17
    Species: Mouse
    Sample type: Plasma
  4. Mao Y., Yen H., Sun Y., Lv Z., Huang R. Development of non-overlapping multiplex antibody arrays for the quantitative measurement of 400 human and 200 mouse proteins in parallel (TECH1P.849). The Journal of ImmunologyMay 1, 2014vol. 192 no. 1 Supplement 69.17
    Species: Mouse
    Sample type: Serum
  5. Lai H, Zhao X, Qin Y, Ding Y, Chen R, Li G, Labrie M, Ding Z, Zhou J, Hu J, Ma D, Fang Y, Gao Q. FAK-ERK activation in cell/matrix adhesion induced by the loss of apolipoprotein E stimulates the malignant progression of ovarian cancer. J Exp Clin Cancer Res. 2018 Feb 20;37(1):32. doi: 10.1186/s13046-018-0696-4. Erratum in: J Exp Clin Cancer Res. 2019 Oct 15;38(1):415. PMID: 29458390; PMCID: PMC5819228.
    Species: Mouse
    Sample type: Conditioned Media
  6. Yariswamy M., Yoshida T., Valente A., et al. Cardiac-restricted Overexpression of TRAF3 Interacting Protein 2 (TRAF3IP2) Results in Spontaneous Development of Myocardial Hypertrophy, Fibrosis, and Dysfunction. J Biol Chem. 2016 Sep 9;291(37):19425-36. doi: 10.1074/jbc.M116.724138
    Species: Mouse
    Sample type: Tissue Lysate (Left Ventricular lysates in a cardiac stress model)
  7. Erikson J., Valente A., Mummidi S., et al. Targeting TRAF3IP2 by genetic and interventional approaches inhibits ischemia/reperfusion-induced myocardial injury and adverse remodeling. J Biol Chem. 2017 Feb 10;292(6):2345-2358. doi: 10.1074/jbc.M116.764522.
    Species: Mouse
    Sample type: Tissue Lysate (Left Ventricular lysates in a cardiac stress model)
  8. Kurtz S., Elkins K. Correlates of vaccine-induced protection against TB immune revealed in comparative analyses of lymphocyte populations. Clinical and Vaccine Immunology, Accepted manuscript posted online 12 August 2015, doi: 10.1128/CVI.00301-15
    Species: Mouse
    Sample type: Conditioned Media (CO coluture of PBMC and splenocytes)
  9. Wang Y, Tan J, Hu P, Pei Q, Wen Y, Ma W, Shi K, Li Z, Li H, Cheng F, Gu X, Yao X, Man Y, Zhao R, Feng S, Ding X, Yang T. Traditional Chinese medicine compound, Bu Sheng Hui Yang Fang, promotes the proliferation of lymphocytes in the immunosuppressed mice potentially by upregulating IL-4 signaling. Biomed Pharmacother. 2020 Dec 16;134:111107. doi: 10.1016/j.biopha.2020.111107. Epub ahead of print. PMID: 33341059.
    Species: Mice
    Sample type: Serum

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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.

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