The JAK/STAT pathway is one of the most important signaling pathways that link extracellular signals to the machinery that controls fundamental cellular processes such as immunity, proliferation, differentiation, apoptosis and oncogenesis.

The RayBio® Mouse JAK/STAT Pathway Phosphorylation Array Kit is a rapid, sensitive, and economical tool for the simultaneous detection of the relative levels of phosphorylation of 12 JAK/STAT pathway proteins. Each antibody was carefully validated using appropriate cell lysates. Using the RayBio® Mouse JAK/STAT Pathway Phosphorylation Array Kit, you can verify multiple specific targets activation in your system without spending excess time and effort in performing an analysis of ELISA and/or Western Blot. The RayBio® Mouse JAK/STAT Pathway Phosphorylation Array Kit has several advantages over the detection of JAK/STAT pathway markers using single-target ELISA kits or Western blots:

1. More Data, Same or Less Sample: Antibody arrays provide high-content screening using about the same sample volume as traditional ELISA.
2. Global View of Protein Expression: Antibody array screening improves the chances for discovering key factors, disease mechanisms, or biomarkers related to cell signaling.
3. Similar (sometimes better) Sensitivity: As little as 4 pg/ml of MCP-1 can be detected using the C-Series array format. In contrast, our similar MCP-1 ELISA assay has a sensitivity of 40 pg/ml of MCP-1.
4. Wider Detection Range: ELISA typically detects a concentration range of 100 to 1000-fold, while RayBiotech arrays can detect concentration ranges up to 10,000-fold.
5. Better Precision: As determined by densitometry, the inter-array Coefficient of Variation (CV) of spot signal intensities is 5-10%, comparing favorably with ELISA testing (CV = 10-15%).

• Easy to use
• No specialized equipment needed
• Compatible with nearly any liquid sample
• Proven technology (many publications)
• Highly sensitive (pg/ml)
• Sandwich ELISA specificity
• Higher density than ELISA, Western blot or bead-based multiplex

For best results, store the entire kit frozen at -20^°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20^°C and all other reagents undiluted at 4^°C for no more than 3 months.