Product Features
- High density arrays
- Unbiased detection through direct biotin labeling of samples
- Low sample consumption
- High detection sensitivity
- Same day result
- Affordable and simple to use
Target Names
AARE, ACAT1, acyl-CoA Thioesterase 2, ADAM28, AHCY, AK1, AKR1A1, ALDH2, alpha 5 D, ANKRD9, Annexin A3, AP180, AP3S2, APLP2, Apolipoprotein A V, ASPM, ASS1, ATOX1, ATPG, AUTS2, BAI2, BarX1, BBS1, BE2I / UBC9, BLM, BOLA2, C10orf58, CACNA1H, Calpain 2, CaMK2, CaMK2D, CBL, CBR1, CCDC58, CCT6A, CHCHD3, Cingulin, CIT, CMG1, CNBP, CNPY2, Coilin, COL8A2, COLEC11, COPG2, CORO1B, CPA3, CPI17 alpha, CrkRS, CRLF3, CSRP3, CTNNAL1, CTNND1, Cyclophilin F, Cytochrome b5, DCAMKL1, Dematin, DIAPH1, DKC1, DLST, DMRT1, Dystrophin, Ebf4, EBP50, ECHDC1, EHHADH, EIF3D, eIF4A2, eIF4GII, ENDOD1, EYA2, Factor VIII, Filaggrin, FITM1, GARS, GCC2, GLI-2, GLOD4, GLUL, GMPR1, GOLGA3, GP2, gp340, GTF2F1, HA1, HARS, HIC1, HIP55, Histone H1.0, Histone H1.5, HIVEP2, hnRNP K, hnRNP R, HNRNPUL2, HNRPA3, HP1 g, IGSF4A, Importin 7, Involucrin, ISLR, ITPR2, ITPR3, KCNAB3, Laminin alpha 5, LDB3, LHPP, LIPG, MAP4K4, MICALL2, MON2, MPST, MRC2, MSH3, MTA2, MTHFD1, MUC5B, MVD, Myosin IIB, NACA1, NAGPA, NAV2, Nectin-4, NFATC4, NNT, NPEPPS, NQO2, NSFL1C, Nucleobindin 1, NUP214, OAF, OIT3, Oncostatin M R beta, OPCML, Orosomucoid 2, OSBP1, OSCAR, Osteoadherin, OTC, OTUB1, OTUD7A, Oxytocin-neurophysin 1, p16 ARC, p23, p39, P4HB, p73, PABP1, PACS1, Pancreatic Lipase, PARVB, PCAP, PCBP1, PCBP2, PCCA, PCDH8, PCK2, PCMT1, PCNA, PCPE-1, PDAP1, PDE1B, PDIA6, PDLIM1, PDLIM3, PDZD2, PEBP1, PEBP4, PENK, PEPD, perilipin 3, Perilipin-1, Periostin, Periplakin, Peroxiredoxin 2, Peroxiredoxin 3, Peroxiredoxin-1, PFAS, PFDN6, PFKL, PGAM1, PGAM2, PGK-1, PGLS, PG-M, PGM1, PGRPL, PHGDH, Piccolo, pIgR, PIK3C2B, PIN, PIP5K2 alpha, PISD, PLA2G1B, Plastin 3, Plastin L, PLBD2, PLD4, Plectin, Plexin B1, Plexin B2, PLOD1, PLOD2, Plxdc2, PMCA, PNP, POLD2, POLR2A, POR, PPOX, PPP1CC, PPP1R9A, PPP2R1B, PPP2R4, PRCP, PRDM13, PREP, PRG2, Prion protein PrP, Profilin 1, Prolargin, Proprotein Convertase 9, Prosaposin, Prostaglandin D Synthase, Proteasome 26S S2, Protein C, Protein Z, Protocadherin-12, PRRC2A, PRSS23, PRSS3, PRTN3, PSMA1, PSMA2, PSMA4, PSMA5, PSMA6, PSMB1, PSMB2, PSMB3, PSMB4, PSMB5, PSMB6, PSMB7, PSMC3, PSMD1, PSMD5, PSMD9, PSME1, PSME2, PTBP1, PTEN, PTGR1, PTK 7, PTMA, PTPRG, PTPRK, PTPRM, PTPRZ, PZP, QARS, QDPR, QPRT, Quiescin Q6, Rab1A, Rab7a, Ran, RanBP1, RanGAP1, RAP1B, Rbm15, RCL, RECQ4, Reg3A, REV3L, RHOC, RHOG, Ribonuclease A, Ribonuclease T2, RLF, RNASE4, Rnose2, RP1, RPL10, RPL10A, RPL11, RPL12, RPL14, RPL17, RPL22, RPL23A, RPL3, RPL32, RPL4, RPL7, RPL7A, RPLP0, RPLP2, RPS10, RPS11, RPS12, RPS13, RPS14, RPS15A, RPS16, RPS18, RPS19, RPS2, RPS20, RPS23, RPS25, RPS3, RPS3A, RPS4X, RPS5, RPS8, RPS9, RREB1, RSF1, RSU1, RUSC1, S E P T 7, S100 A1, S100A11, S100A7, S100A9, SAA, SAA4, SBP-1, SC35, SCG, SCN3A, SCP2, SDNSF, SDPR, SECISBP2, Secretogranin V, Semaphorin 6B, Semaphorin 7A, SERBP1, Serpin A11, Serpin A7, Serpin B3D, Serpin B6, Serpin B8, Serpin F2, Serpin H1, Serpina10, SERPINB1, SerpinB4, SerpinE2, SerRS, SET, SEZ6L2, SF20, SHANK1, SHC1, SHMT1, SHOX, SHP-1, Siglec-1, SIM2, SIRPB1 , Six3, SLC4A1, SLITRK1, SLURP1, SMAD6, SMC4, SMPD4, SNRPD1, SOD1, SOD2, SOD-3, Somatoliberin, Somatostatin, SORD, SorLA, SOX4, SOX5, SP-D, Spectrin, SPEN, SPG48, SPINK5, SPS2L, SPTBN2, SPTLC1, Src, SSC5D, STAT3, Stathmin 1, STI1, STOM, STXBP2, SUCLG1, SUMO3, SVEP1, Symplekin, Synemin, SYNPO2L, Syntaxin 7, TAB182, Talin1, TARS, TAX1BP3, TBCA, TCEB2, Tcf20, TCP1 delta, TCP1 eta, TCP1 theta, TCTP, TDIF2, Tenascin C, Tenascin XB, TFF2, TGM3, Thimet Oligopeptidase, Thioredoxin-1, TIF1 alpha, TMEM103, TOB2, TOMM70A, TOP2B, TPD52L2, TPM4, TPP1, TPPP3, TPR, Transaldolase 1/TALDO1, Transthyretin, TRAP1, TRAP220, TRF 2, TRIM14, Tropomyosin 3, TRP-1, TRPS1, Trypsinogeb-2, TSR2, TTC3, TTF1, TUBA6, TWF2, TXNDC15, TXNDC4, TXNDC5, TXNRD2, UBA1, UBE2D3, Ube2L3, UBE2N/Ubc13, UCH-L1, UFM 1, UGGT, UMP/CMP, UNC13 Homolog D, UNC45A, UNC5H4, UPB1, UQCRB, UQCRH, URB, URB2, UROC1, UROD, Uroguanylin, URP2, USP14, USP2, USP5, Uteroglobin, Utrophin, valyl tRNA, VAP-1, VAP-A, VCP, VDAC1, VILIP3, Vimentin, VNN1, VPS4B, VSIG4, WDR1, WDR44, WISP2, WNK2, XPG, YB1, YN1 / Synapsin 1, YY1, ZAK, zbtb11, ZBTB4, ZC3H18, ZC3H4-N-t, ZC3H8, ZNF295, Zyxin
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process; Cross-species testing
Kit Components
- RayBio® L-Series Antibody Array Membranes
- Labeling Reagent
- Stop Solution
- Blocking Buffer
- Detection Buffer C
- Detection Buffer D
- Wash Buffer 1
- Wash Buffer 2
- HRP-Conjugated Streptavidin Concentrate
- Plastic Sheets
- Spin Columns
- Labeling Buffer
- Lysis Buffer
- Plastic Incubation Tray
Other Materials Required
- Distilled or de-ionized water
- Small plastic or glass containers
- Orbital shaker or oscillating rocker
- Pipettors, pipette tips and other common lab consumables
- 2?5 ml tube
- 15 ml conical collection tube
- Tissue Paper, blotting paper or chromatography paper
- A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Purify Samples with Spin Column
- Biotinylate Samples
- Purify biotinylated samples using spin column
- Block array membranes
- Incubate with Samples
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store Box 1 frozen at -20°C and Box 2 at 4°C upon arrival. Stored this way the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes at -20°C and all other reagents undiluted at 4°C for no more than 3 months.