| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Serratia marcescens |
| Sensitivity | 19.92 pg/mL |
| Detection Range | 19.92 - 9600 pg/mL |
| Specificity | This ELISA kit has been validated with SuperNuclease®, Benzonase® and RayBio® Universal Nuclease, and should react with other Serratia marcescens nuclease products but not tested. Both non-denatured and denatured nuclease can be detected by this kit. |
| Compatible Sample Types | Cell Culture Supernatants |
| Accession Number | P13717 |
| Gene Symbols | nucA nuc |
| Protein Name / Synonyms | Nuclease (EC 3.1.30.2) (Endonuclease) [Cleaved into: Nuclease isoform Sm2; Nuclease isoform Sm3; Nuclease isoform Sm1] |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Bioprocesses |
| Shipping Type | Blue ice |
| Storage | -20°C |
| Sample Type | Average % Recovery | Range (%) |
|---|---|---|
| PBS | 123.2 | 100-145 |
| LB media | 124.0 | 114-135 |
| Cell culture media | 105.7 | 84-138 |
| Sample Type | PBS | LB Media | Cell Culture Media | |
|---|---|---|---|---|
| 1:2 | Average % of Expected | 134.3 | 105.8 | 106.7 |
| Range (%) | 130-138 | 97-115 | 92-134 | |
| 1:4 | Average % of Expected | 114.5 | 81.2 | 88.5 |
| Range (%) | 110-122 | 74-90 | 83-104 | |
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Nuclease ELISA kit Standard Curve
Nuclease ELISA kit Standard Curve