Pembrolizumab (Keytruda®) immunoassay kit enabling sensitive quantification of drug concentrations across serum, plasma, and cell culture supernatants.
Loading...
Loading...
Loading...
Loading...
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 3-5 business days |
| Sensitivity | 7 pg/ml |
| Detection Range | 0.074 ng/ml - 54 ng/ml |
| Specificity | This ELISA antibody pair detects Pembrolizumab. Other targets not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 10 fold |
| Protein Name / Synonyms | Pembrolizumab |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Antibody Drug Development, Drug Antibody (DA) Detection |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Standard Curve
Kit Components
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- HPR-conjugated Detection Antibody
- Wash Buffer
- Assay Diluent
- TMB One-Step Substrate Reagent
- Stop Solution
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Horizontal orbital microplate shaker
- Tubes to prepare standard or sample dilutions
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µL of each standard and sample to each well. Incubate 2 hours at room temperature with gentle shaking.
- Add 100 μL prepared detection antibody to each well. Incubate for 1 hour at room temperature with gentle shaking.
- Add 100 μL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 μL Stop Solution to each well. Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
