• Pembrolizumab Conjugated-RayPlex® Beads
• 1X Assay Diluent
• 20X Wash Buffer
• Liquid ADA Standard
• Biotinylated Pembrolizumab
• 10X PE Conjugate
• Flow Cytometer Setup Beads
• V-bottom 96-well Microplate
• Filter 96-well Microplate
• Manual

• Orbital 96-well plate shaker (with ability to reach 1000 rpm)
• Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
• Aluminum foil or 96-well Plate Aluminum Sealers
• Distilled water
• 1.5 ml polypropylene microcentrifuge tubes or similar
• Microcentrifuge
• If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
• Optional: 96-well round bottom plate (to prepare samples)
• Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex bead arrays. Get a quote!