The RayBiotech Cell-Based phosphorylation ELISA kit is a rapid, convenient and sensitive cell-based ELISA that can monitor the activation or function of important biological pathways in cells. It can be used for measuring the relative amount of protein phosphorylation and screening the effects of various treatments, inhibitors (such as siRNA or chemicals), or activators in cultured human, mouse and rat cell lines. By determining protein phosphorylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort in preparing cell lysate and performing an analysis of Western Blot.

In the Cell-Based phosphorylation ELISA kit, cells are seeded into a 96 well tissue culture plate. The cells are fixed after various treatments, inhibitors or activators. After blocking, anti-phospho or anti-pan antibodies are pipetted into the wells and incubated. The wells are washed and HRP-conjugated anti-IgG is added to the corresponding wells. The wells are washed again, a TMB substrate solution is added to the wells and color develops in proportion to the amount of protein. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

How It Works