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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Estimated Lead Time | 1-2 business days |
| Species | Staphylococcus spp. |
| Sensitivity | 8.92 pg/ml |
| Detection Range | 8.92 pg/ml - 7500 pg/mL |
| Specificity | This ELISA antibody pair detects Protein A. Other targets not determined. |
| Compatible Sample Types | Cell Culture Supernatants, Cell Lysates |
| Accession Number | P02976 |
| Gene Id | 3919448 |
| Gene Symbols | spa SAOUHSC_00069 |
| Protein Name / Synonyms | Immunoglobulin G-binding protein A (IgG-binding protein A) (Staphylococcal protein A) (SpA) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Bioprocesses |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curve
Spiking & Recovery Results
| Sample Type | Average % Recovery | Range (%) |
|---|---|---|
| PBS | 105 | 94-116 |
Linearity Results
| Sample Type | PBS | Biosimilar Antibodies | |
|---|---|---|---|
| 1:2 | Average % of Expected | 105.6 | 163.4 |
| Range (%) | 99-112 | 136-184 | |
| 1:4 | Average % of Expected | 68.3 | 160.8 |
| Range (%) | 64-73 | 124-185 | |
Kit Components
- Pre-Coated 96-well Strip Microplate
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- Wash Buffer
- TMB One-Step Substrate Reagent
- Stop Solution
- Assay Diluent(s)
Additional Materials Required
- Microplate reader capable of measuring absorbance at 450 nm
- Precision pipettes to deliver 2 µL to 1 µL volumes
- Adjustable 1-25 µL pipettes for reagent preparation
- 100 µL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for ELISA data analysis
- Tubes to prepare standard and sample dilutions
Assay Procedure Summary
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µL standard or sample to each well. Incubate 2.5 hours at room temperature.
- Add 100 µL prepared biotin antibody to each well. Incubate 1 hour at room temperature.
- Add 100 µL prepared Streptavidin solution to each well. Incubate 45 minutes at room temperature.
- Add 100 µL TMB One-Step Substrate Reagent to each well. Incubate 30 minutes at room temperature.
- Add 50 µL Stop Solution to each well. Read at 450 nm immediately
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Protein A ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified Staphylococcal protein A / SpA, including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.