Telomeres are repetitive nucleotide sequences located at the ends of chromosomes, and they are important for chromosome stability. Telomeres shorten in length during each cycle of cell division. Extensive telomere shortening could lead to genomic instability and has implications in various diseases, including cancer and neurodegenerative disorders. Telomere length also serves as a biomarker for aging and age-related diseases. Therefore, the ability to accurately measure telomere length is critical when studying such diseases.

The RayBio^® Rat Telomere Length Absolute Quantification Kit is a Taqman™ probe-based qPCR assay for the specific quantitative measurement of Rat telomere length. The use of Taqman™ probes allows the internal gene (Single copy reference gene, SCR) and telomere (TEL) to be amplified in one reaction tube. The optimized PCR formulation provides superior specificity and efficiency with a wide linear dynamic range. This kit allows for the absolute quantification of telomere length using genomic DNA from various sources including cultured cells, tissue, saliva, scraped cells, urine, spinal fluid, lavage fluid, blood, etc.

• No off-target amplification on nuclear DNA
• Superior specificity and efficiency
• Wide linear dynamic range
• Measures mtDNA in one reaction
• Compatible with various mouse samples

• Fluorescence qPCR instrument capable of reading FAM (494 nm maximum absorption, 518 nm maximum emission) and VIC (532 nm maximum absorption, 552 nm maximum emission) channels
• Sterile nuclease-free pipette tips (barrier tips recommended)
• Microfuge tubes
• Compatible PCR Plate or PCR tubes
• Microcentrifuge
• Vortex Mixer

Contact our technical support team for questions about compatibility: techsupport@raybiotech.com

1. Prepare all reagents, samples and standards as instructed.
2. Load the PCR reaction mix and samples.
3. Seal the PCR reaction tubes or plate.
4. Set up the PCR Amplification.
5. Export the results.
6. Calculate Telomere Length.

CV <3% for Cq value and CV <10% for Copies/Cell when the input DNA >0.1 ng/reaction.

The kit can be stored at -20°C for a period of 12 months prior to opening. After opening, the reagents are valid for at least 6 months if stored at -20°C. Avoid repeated freeze-thaw cycles.