Product Features
- Less sample, more data: up to 12 proteins can be quantified from only 25 µl or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Target Names
CXCL13 (BLC), CCL17 (TARC), CCL20 (MIP-3α), CXCL1 (KC), CXCL9 (MIG), CCL5 (RANTES), CCL2 (MCP-1), CXCL11 (I-TAC), CCL4 (MIP-1β), CX3CL1 (Fractalkine), CCL22 (MDC), CCL11 Eotaxin-1)
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- RayPlex™ Multiplex Bead Cocktail
- 1X Assay Diluent
- 20X Wash Buffer
- RayBio® Serum Diluent
- Lyophilized Protein Standard Mix
- 1X Biotinylated Detection Antibody Cocktail
- 10X PE Conjugate
- Flow Cytometer Setup Bead Cocktail
- V-bottom 96-well Microplate
- Filter 96-well Microplate
Other Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- 1.5 ml polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!
Protocol Outline
- Prepare Reagents, Standards, and Samples
- Add RayPlex™ Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 5) should be stored at -20°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.