Product Description

Specifications

Size	48 tests, 96 tests, 192 tests, 480 tests
Estimated Lead Time	1-2 business days
Species	Porcine (pig)
Specificity	No detectable or significant cross reactivity was detected between antibodies or targets.
Compatible Sample Types	Cell Culture Supernatants, Serum
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	12
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Research Area	Inflammation
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

Product Features

Less sample, more data: up to 12 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
IFNγ Gene Symbol: IFNG Gene ID: 396991 Accession #: P17803 ELISA Kit: Porcine IFN-gamma ELISA Kit	IL-1β Gene Symbol: IL1B Gene ID: 397122 Accession #: P26889 ELISA Kit: Porcine IL-1 beta ELISA Kit	IL-6 Gene Symbol: IL6 Gene ID: 399500 Accession #: P26893 ELISA Kit: Porcine IL-6 ELISA Kit	TNFα Gene Symbol: TNF TNFA TNFSF2 Gene ID: 397086 Accession #: P23563 ELISA Kit: Porcine TNF-alpha ELISA Kit	IL-17A Gene Symbol: IL17A IL17 Gene ID: 449530 Accession #: Q60I29 ELISA Kit: Porcine IL-17 ELISA Kit
IL-10 Gene Symbol: IL10 Gene ID: 397106 Accession #: Q29055 ELISA Kit: Porcine IL-10 ELISA kit	IL-12p40 Gene Symbol: IL12B Gene ID: 397076 Accession #: Q28938 ELISA Kit: Porcine IL-12 p40 ELISA Kit	IL-4 Gene Symbol: IL4 Gene ID: 397225 Accession #: Q04745 ELISA Kit: Porcine IL-4 ELISA Kit	IL-2 Gene Symbol: IL2 Gene ID: 396868 Accession #: P26891 ELISA Kit: Porcine IL-2 ELISA Kit	IL-13 Gene Symbol: IL13 Gene ID: 396721 Accession #: Q95J68 ELISA Kit: Porcine IL-13 ELISA Kit
IL-8 Gene Symbol: CXCL8 IL8 Gene ID: 396880 Accession #: P26894 ELISA Kit: Porcine IL-8 ELISA Kit	MCP-1 Gene Symbol: CCL2 SCYA2 Gene ID: 397422 Accession #: P42831

Application Notes

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
Flat-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Product Features

Less sample, more data: up to 12 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
IFNγ Gene Symbol: IFNG Gene ID: 396991 Accession #: P17803 ELISA Kit: Porcine IFN-gamma ELISA Kit	IL-1β Gene Symbol: IL1B Gene ID: 397122 Accession #: P26889 ELISA Kit: Porcine IL-1 beta ELISA Kit	IL-6 Gene Symbol: IL6 Gene ID: 399500 Accession #: P26893 ELISA Kit: Porcine IL-6 ELISA Kit	TNFα Gene Symbol: TNF TNFA TNFSF2 Gene ID: 397086 Accession #: P23563 ELISA Kit: Porcine TNF-alpha ELISA Kit	IL-17A Gene Symbol: IL17A IL17 Gene ID: 449530 Accession #: Q60I29 ELISA Kit: Porcine IL-17 ELISA Kit
IL-10 Gene Symbol: IL10 Gene ID: 397106 Accession #: Q29055 ELISA Kit: Porcine IL-10 ELISA kit	IL-12p40 Gene Symbol: IL12B Gene ID: 397076 Accession #: Q28938 ELISA Kit: Porcine IL-12 p40 ELISA Kit	IL-4 Gene Symbol: IL4 Gene ID: 397225 Accession #: Q04745 ELISA Kit: Porcine IL-4 ELISA Kit	IL-2 Gene Symbol: IL2 Gene ID: 396868 Accession #: P26891 ELISA Kit: Porcine IL-2 ELISA Kit	IL-13 Gene Symbol: IL13 Gene ID: 396721 Accession #: Q95J68 ELISA Kit: Porcine IL-13 ELISA Kit
IL-8 Gene Symbol: CXCL8 IL8 Gene ID: 396880 Accession #: P26894 ELISA Kit: Porcine IL-8 ELISA Kit	MCP-1 Gene Symbol: CCL2 SCYA2 Gene ID: 397422 Accession #: P42831

Application Notes

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
Flat-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

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Specifications

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Storage/Stability

Documents

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