Product Features
- Less sample, more data: up to 12 proteins can be quantified from only 25 µl or less of your precious sample
- 4 hour processing time
- 5-10x more cost-effective than ELISA
- Compatible with most flow cytometers
- No specialized or dedicated instrument(s) needed
- High specificity and system reproducibility
- Well-suited for high throughput assays
Target Names
IFN-γ, IL-10, IL-17, IL-2, IL-1β, TNF-α, Eotaxin-1(CCL11), IL-13, KC (CINC-1), IL-2 R alpha, IL-6, IL-4
Kit Components
- RayPlex® Magnetic Multiplex Bead Cocktail
- Assay Diluent
- Wash Buffer
- RayBio® Serum Diluent
- Lyophilized Protein Standard Mix
- Biotinylated Detection Antibody Cocktail
- PE Conjugate
- Flow Cytometer Setup Bead Cocktail
- Flat-bottom 96-well Microplate
- V-bottom 96-well Microplate
Additional Materials Required
- Orbital 96-well plate shaker (with ability to reach 1000 rpm)
- Flow Cytometer with blue (or green) and red lasers capable of measuring phycoerythrin (PE), Alexa Fluor 700 (AF700), and Alexa Fluor 647 (AF647)/APC channels OR Luminex instrument
- Aluminum foil or 96-well Plate Aluminum Sealers
- Distilled water
- PBS
- 1.5 mL polypropylene microcentrifuge tubes or similar
- Microcentrifuge
- Recommended: Automated plate washing system with magnetic separation
- Optional: 96-well round bottom plate (to prepare samples)
- Optional: High throughput sampler (HTS) for 96-well plate reading
- Optional: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate
Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!
Assay Procedure Summary
- Prepare Reagents, Standards, and Samples
- Add MagPro RayPlex® Multiplex Bead Cocktail to each well
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluorophore
- Analyze with a compatible flow cytometer
- Perform data analysis
Storage/Stability
Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.