• Less sample, more data: up to 12 proteins can be quantified from only 25 µl or less of your precious sample
• 4 hour processing time
• 5-10x more cost-effective than ELISA
• Compatible with most flow cytometers
• No specialized or dedicated instrument(s) needed
• High specificity and system reproducibility
• Well-suited for high throughput assays

IFN-γ, IL-10, IL-17, IL-2, IL-1β, TNF-α, Eotaxin-1(CCL11), IL-13, KC (CINC-1), IL-2 R alpha, IL-6, IL-4

• RayPlex^® Magnetic Multiplex Bead Cocktail
• Assay Diluent
• Wash Buffer
• RayBio^® Serum Diluent
• Lyophilized Protein Standard Mix
• Biotinylated Detection Antibody Cocktail
• PE Conjugate
• Flow Cytometer Setup Bead Cocktail
• Flat-bottom 96-well Microplate
• V-bottom 96-well Microplate

• Orbital 96-well plate shaker (with ability to reach 1000 rpm)
• Flow Cytometer with blue (or green) and red lasers capable of measuring phycoerythrin (PE), Alexa Fluor 700 (AF700), and Alexa Fluor 647 (AF647)/APC channels OR Luminex instrument
• Aluminum foil or 96-well Plate Aluminum Sealers
• Distilled water
• PBS
• 1.5 mL polypropylene microcentrifuge tubes or similar
• Microcentrifuge
• Recommended: Automated plate washing system with magnetic separation
• Optional: 96-well round bottom plate (to prepare samples)
• Optional: High throughput sampler (HTS) for 96-well plate reading
• Optional: Vacuum manifold compatible with a 96-well microplate if using a Filter 96-Well Microplate

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

1. Prepare Reagents, Standards, and Samples
2. Add MagPro RayPlex^® Multiplex Bead Cocktail to each well
3. Incubate with Samples and Standards
4. Incubate with Biotinylated Detection Antibody Cocktail
5. Incubate with Streptavidin-Conjugated Fluorophore
6. Analyze with a compatible flow cytometer
7. Perform data analysis

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.