Biotin-conjugated recombinant Human EGFR, N-terminal extracellular domain, derived from the transfected human HEK293 cells
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Product Description
Specifications
| Size | 10 µg, 50 µg, 100 µg, 250 µg |
| Estimated Lead Time | 2-3 business days |
| Species | Human |
| Accession Number | P00533 |
| Gene Id | 1956 |
| Gene Symbols | EGFR |
| Protein Name / Synonyms | Epidermal Growth Factor Receptor, Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian), ERBB, PIG61, Cell Growth Inhibiting Protein 40, ERBB1, Cell Proliferation-Inducing Protein 61, HER1, mENA, Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1 |
| Expressed Region | Leu25-Ser645 |
| Expression System | HEK293 cells |
| Conjugation | Biotin |
| Tag | N-terminal 8x histidine tag. The primary amino groups (-NH2) at the N-terminus and the side chains of lysine (K) residues were biotin-conjugated using the standard chemical labeling method. |
| Purity | >90% |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | The recombinant human EGFR extracellular domain has a calculated molecular mass of 71 kDa. Due to the abundant glycosylation, it migrates as approximately 100 kDa protein bands (confirmed by Western blotting) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. |
| Format | Lyophilized powder |
| Formulation | Lyophilized from a 0.2 µm filtered solution in PBS |
| Concentration (lot specific) | Determined by BCA protein assay kit (Thermo Scientific) |
| Preservative | None |
| Reconstitution | Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS. |
| Endotoxin Level | Not determined |
| Research Area | Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling, Tyrosine Kinase Signaling |
| Protein Type | Recombinant Protein |
| Shipping Type | Ambient temperature |
| Storage | -20°C |
SDS-PAGE Image
Activity
Storage/Stability
Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.
