Recombinant Human EGFR (ERBB1/HER1) Protein

Recombinant Human EGFR, N-terminal extracellular domain, derived from the transfected human HEK293 cells

Product Description

Specifications

Size	10 µg, 50 µg, 100 µg
Estimated Lead Time	1-2 business days
Species	Human
Accession Number	P00533
Gene Id	1956
Gene Symbols	EGFR
Protein Name / Synonyms	Epidermal Growth Factor Receptor, Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog (Avian), ERBB, PIG61, Cell Growth Inhibiting Protein 40, ERBB1, Cell Proliferation-Inducing Protein 61, HER1, mENA, Avian Erythroblastic Leukemia Viral (V-Erb-B) Oncogene Homolog, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
Expressed Region	Leu25-Ser645
Expression System	HEK293 cells
Tag	N-terminal 6x histidine tag
Purity	> 90%
Purity Determined By	SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Molecular Weight (kDa)	The recombinant human EGFR extracellular domain has a calculated molecular mass of 71 kDa. Due to the abundant glycosylation, it migrates as approximately 100 kDa protein bands (confirmed by Western blotting) in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions.
Format	Lyophilized powder
Formulation	Lyophilized from a 0.2 µm filtered solution in PBS
Concentration (lot specific)	Determined by BCA protein assay kit (Thermo Scientific)
Preservative	None
Reconstitution	Briefly spin the vial and bring the contents to the bottom prior to opening. It is recommended to reconstitute at 0.5 - 1.0 mg/mL with sterile deionized water or 1x PBS.
Endotoxin Level	Not determined
Research Area	Cardiovascular Disease, Cancer, Inflammation, HER/ErbB Signaling, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding, Tyrosine Kinase Signaling
Shipping Type	Ambient temperature
Storage	-20°C

Introduction

Epidermal Growth Factor Receptor (EGFR), also known as ErbB1 or HER1, is a transmembrane glycoprotein that belongs to the ErbB family of receptor tyrosine kinases. It is widely expressed in various tissues and plays a critical role in regulating cell proliferation, differentiation, and survival. Upon binding to its ligands, such as epidermal growth factor, EGFR undergoes dimerization and autophosphorylation, initiating multiple downstream signaling pathways. Aberrant EGFR signaling, due to overexpression or mutations, is implicated in the development and progression of various cancers, making it a prominent target for anticancer therapies.

Reference: https://pubmed.ncbi.nlm.nih.gov/22785351/

SDS-PAGE Image

Activity

Measured by its binding ability in a functional ELISA. Immobilized recombinant EGFR can bind anti-human EGFR antibody drug Panitumumab with EC50 at 2.2 ng/mL.

Storage/Stability

Upon arrival, the lyophilized protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store desiccated below -20°C in a manual defrost freezer. Following reconstitution, the protein may be stored for 2 weeks under sterile conditions at -20°C. For long term storage, it is recommended to make appropriate aliquots and store at -80°C. Avoid repeated freeze-thaw cycles.