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Bridging ELISA Kits

Quantitative Sandwich ELISAs for Drug Antibody (DA) or Anti-Drug Antibody (ADA) Detection

Bridging ELISAs are a type of sandwich ELISA designed for detection of antigens with 2 or more identical binding sites, resulting in the formation of a "bridge" complex (see Fig.1). This format is commonly used for quantification of therapeutic antibodies; additionally, anti-drug antibodies resulting from immune response to a drug can be detected. The bridging ELISA technique is ideally suited development of drug antibodies (DA), since it enables pharmacokinetic characterization as well as detection of immunogenicity.

Like all RayBio ELISA kits, our bridging sandwich ELISAs are manufactured in Peachtree Corners, GA, backed by a 100% satisfaction guarantee, and are part of a comprehensive menu of competitively priced products and services to advance AD development and discovery.

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Bridging complex illustation

How It Works

Bridging ELISA drug antibody detection
A. How RayBio ELISA detects DA: RayBio DA ELISA employs an antibody specific for the drug coated on a 96-well plate. Standards and samples are pipetted into the wells; the drug molecule binds to the immobilized antibody. The wells are washed and biotinylated anti-drug antibody is then added. After washing away unbound biotinylated antibody, HRP- conjugated streptavidin is added to the wells, followed by a substrate solution. The intensity of color development in the wells is proportional to the amount of DA bound.
Bridging ELISA anti-drug antibody detection
B. How RayBio ELISA detects ADA: RayBio ADA ELISA employs a DA coated on a 96-well plate. Standards and samples are pipetted into the wells; the anti-drug antibodies present in the sample bind to the immobilized DA. The wells are washed and HRP-conjugated detection antibody is then added, followed by a substrate solution. The intensity of color development in the wells is proportional to the amount of ADA bound.

Features:

  • Reliable, robust sandwich ELISA format
  • Highly sensitive and specific
  • Assays available for many FDA-approved biologics
  • Compatible with serum, plasma, and many other sample types
  • Custom kits available (contact us for a quote)

Drug Development Applications:

  • Quantifying DA in a variety of matrices
  • Pharmacokinetic analysis
  • Immunogenicity characterization
  • Detection of unwanted immune responses to biotherapeutics

Contents of Kit

  • 96-well pre-coated strip plate (12x8)
  • Wash Buffer Concentrate (20x)
  • DA or ADA Standard
  • Sample Diluent Buffers
  • Labeled Detection Antibody
  • HRP-Streptavidin concentrate*
  • TMB One-Step Substrate Reagent
  • Stop Solution

* in select kits only