Product Description

Specifications

Size	2 Sample Kit, 4 Sample Kit, 8 Sample Kit
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates
Quantitative/Semi-Quantitative	Semi-Quantitative
Number of Targets Detected	120
Recommended Applications	Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Solid Support	Membrane
Design Principle	Sandwich-based
Method Of Detection	Chemiluminescence
Shipping Type	Blue ice
Storage	-20°C

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin (ACRP30) Gene Symbol: ADIPOQ Gene ID: 9370 Accession #: Q15848 ELISA Kit: RayBio Human Acrp30 ELISA	AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	Amphiregulin Gene Symbol: AREG Gene ID: 374 Accession #: P15514 ELISA Kit: RayBio Human AREG ELISA	Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123 ELISA Kit: RayBio Human ANGPT2 ELISA
Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530 ELISA Kit: RayBio Human Axl ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA	bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	BMP-4 Gene Symbol: BMP4 Gene ID: 652 Accession #: P12644 ELISA Kit: RayBio Human BMP-4 ELISA
BMP-6 Gene Symbol: BMP6 Gene ID: 654 Accession #: P22004 ELISA Kit: RayBio Human BMP-6 ELISA	beta-NGF Gene Symbol: NGF Gene ID: 4803 Accession #: P01138 ELISA Kit: RayBio Human beta-NGF ELISA	Betacellulin (BTC) Gene Symbol: BTC Gene ID: 685 Accession #: P35070 ELISA Kit: RayBio Human BTC ELISA	CCL28 (MEC) Gene Symbol: CCL28 Gene ID: 56477 Accession #: Q9NRJ3 ELISA Kit: RayBio Human CCL28 ELISA	CK beta 8-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human Ckbeta8 ELISA
CNTF Gene Symbol: CNTF Gene ID: 1270 Accession #: P26441 ELISA Kit: RayBio Human CNTF ELISA	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: 10850 Accession #: Q9Y4X3 ELISA Kit: RayBio Human CTACK ELISA	Dtk Gene Symbol: TYRO3 Gene ID: 7301 Accession #: Q06418 ELISA Kit: RayBio Human Dtk ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human EGFR ELISA
ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA
FGF-4 Gene Symbol: FGF4 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA	FGF-6 Gene Symbol: FGF6 Gene ID: 2251 Accession #: P10767 ELISA Kit: RayBio Human FGF-6 ELISA	FGF-7 (KGF) Gene Symbol: FGF7 Gene ID: 2252 Accession #: P21781 ELISA Kit: RayBio Human FGF-7 ELISA	FGF-9 Gene Symbol: FGF9 Gene ID: 2254 Accession #: P31371 ELISA Kit: RayBio Human FGF-9 ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Gene ID: 2323 Accession #: P49771 ELISA Kit: RayBio Human Flt-3 L ELISA
Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Gene ID: 6376 Accession #: P78423 ELISA Kit: RayBio Human Fractalkine ELISA	GCP-2 (CXCL6) Gene Symbol: CXCL6 Gene ID: 6372 Accession #: P80162 ELISA Kit: RayBio Human GCP-2 ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GDNF Gene Symbol: GDNF Gene ID: 2668 Accession #: P39905 ELISA Kit: RayBio Human GDNF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Gene ID: 8784 Accession #: Q9Y5U5 ELISA Kit: RayBio Human GITR ELISA
GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Gene ID: 8995 Accession #: Q9UNG2 ELISA Kit: RayBio Human GITR L ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	GRO alpha/beta/gamma Gene Symbol: CXCL1/CXCL2/CXCL3 Gene ID: 2919/2920/2921 Accession #: P09341/P19875/P19876 ELISA Kit: RayBio	GRO alpha (CXCL1) Gene Symbol: CXCL1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA	HCC-4 (CCL16) Gene Symbol: CCL16 Gene ID: 6360 Accession #: O15467 ELISA Kit: RayBio Human HCC-4 ELISA
HGF Gene Symbol: HGF Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	ICAM-3 (CD50) Gene Symbol: ICAM3 Gene ID: 3385 Accession #: P32942 ELISA Kit: RayBio Human ICAM-3 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA
IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: 3489 Accession #: P24592 ELISA Kit: RayBio Human IGFBP-6 ELISA
IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human IGF-1 R ELISA	IL-1 R4 (ST2) Gene Symbol: IL1RL1 Gene ID: 9173 Accession #: Q01638 ELISA Kit: RayBio Human IL-1 R4 ELISA	IL-1 R1 Gene Symbol: IL1R1 Gene ID: 3554 Accession #: P14778 ELISA Kit: RayBio Human IL-1 sRI ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA
IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA
IL-16 Gene Symbol: IL16 Gene ID: 3603 Accession #: Q14005 ELISA Kit: RayBio Human IL-16 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552 ELISA Kit: RayBio Human IL-17A ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-1 ra (IL-1 F3) Gene Symbol: IL1RN Gene ID: 3557 Accession #: P18510 ELISA Kit: RayBio Human IL-1 RA ELISA
IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-2 R alpha Gene Symbol: IL2RA Gene ID: 3559 Accession #: P01589 ELISA Kit: RayBio Human IL-2 R alpha ELISA	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: RayBio Human IL-3 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA
IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625 ELISA Kit: RayBio Human I-TAC ELISA
Leptin Gene Symbol: LEP Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA	Light (TNFSF14) Gene Symbol: TNFSF14 Gene ID: 8740 Accession #: O43557 ELISA Kit: RayBio Human LIGHT ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: 6375 Accession #: P47992 ELISA Kit: RayBio Human Lympotactin ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-2 (CCL8) Gene Symbol: CCL8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA
MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	MCP-4 (CCL13) Gene Symbol: CCL13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	MDC (CCL22) Gene Symbol: CCL22 Gene ID: 6367 Accession #: O00626 ELISA Kit: RayBio Human MDC ELISA	MIF Gene Symbol: MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA
MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 Gene ID: 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-1 delta (CCL15) Gene Symbol: CCL15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA
MIP-3 beta (CCL19) Gene Symbol: CCL19 Gene ID: 6363 Accession #: Q99731 ELISA Kit: RayBio Human MIP-3 beta ELISA	MSP alpha/beta Gene Symbol: MST1 Gene ID: 4485 Accession #: P26927 ELISA Kit: RayBio Human MSP ELISA	NAP-2 (PPBP/CXCL7) Gene Symbol: Ppbp Gene ID: 5473 Accession #: P02775 ELISA Kit: RayBio Human NAP-2 ELISA	NT-3 Gene Symbol: NTF3 Gene ID: 4908 Accession #: P20783 ELISA Kit: RayBio Human NT-3 ELISA	NT-4 Gene Symbol: NTF4 Gene ID: 4909 Accession #: P34130 ELISA Kit: RayBio Human NT-4 ELISA
Oncostatin M Gene Symbol: OSM Gene ID: 5008 Accession #: P13725 ELISA Kit: RayBio Human OSM ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	PARC (CCL18) Gene Symbol: CCL18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	PLGF Gene Symbol: PGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA
RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	SCF Gene Symbol: KITLG Gene ID: 4254 Accession #: P21583 ELISA Kit: RayBio Human SCF ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA	gp130 Gene Symbol: Il6st Gene ID: 3572 Accession #: P40189 ELISA Kit: RayBio Human sgp130 ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA
TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA	TARC (CCL17) Gene Symbol: CCL17 Gene ID: 6361 Accession #: Q92583 ELISA Kit: RayBio Human TARC ELISA	TECK (CCL25) Gene Symbol: CCL25 Gene ID: 6370 Accession #: O15444 ELISA Kit: RayBio Human TECK ELISA	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA
Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA	TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA
TRAIL R3 (TNFRSF10C) Gene Symbol: TNFRSF10C Gene ID: 8794 Accession #: O14798 ELISA Kit: RayBio Human TRAIL R3 ELISA	TRAIL R4 (TNFRSF10D) Gene Symbol: TNFRSF10D Gene ID: 8793 Accession #: Q9UBN6 ELISA Kit: RayBio Human TRAIL R4 ELISA	uPAR Gene Symbol: PLAUR Gene ID: 5329 Accession #: Q03405 ELISA Kit: RayBio Human uPAR ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA	VEGF-D Gene Symbol: FIGF Gene ID: 2277 Accession #: O43915 ELISA Kit: RayBio Human VEGF-D ELISA

Typical Data

RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to profile secreted proteins from LILRB4+ versus LILRB4-knockout AML cells, revealing reduced uPAR expression upon LILRB4 deletion. Figure adapted from Deng et al., Nature, 2018.

RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to compare secreted proteins from ghMSCs and hMSCs, revealing higher levels of IL-8, GRO chemokines, MCP-1, VEGF, IGFBP-4, and HGF in ghMSCs. Figure adapted from Lee et al., Molecular Therapy, 2025.

RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to compare secreted proteins from untreated and cisplatin-treated cancer-associated fibroblasts (CAFs), revealing increased secretion of HGF, IL-1α, MCP-1, SDF-1, and angiogenin in response to chemotherapy. Figure adapted from Palliyage et al., Lung Cancer, 2023.

RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to profile TNF-α–induced chemokine secretion in triple-negative breast cancer cells, revealing that thymoquinone reduced CCL2, CCL3, CCL4, and CCL20 expression. Figure adapted from Adinew et al., International Journal of Molecular Sciences, 2023.

RayBiotech’s Human Cytokine Array C1000 (Cat# AAH-CYT-1000) was used to assess cytokine secretion from macrophages cultured on titanium surfaces with varying coatings, revealing surface-dependent changes in immune signaling. Figure adapted from Huang et al., Bioactive Materials, 2023.

Kit Components

Human Cytokine Antibody Array C1000 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.

Product Features

Easy to use
No specialized equipment needed
Compatible with nearly any liquid sample
Proven technology (many publications)
Highly sensitive (pg/ml)
Sandwich ELISA specificity
Higher density than ELISA, Western blot or bead-based multiplex

Target Names

Scroll over each target protein for more information
Adiponectin (ACRP30) Gene Symbol: ADIPOQ Gene ID: 9370 Accession #: Q15848 ELISA Kit: RayBio Human Acrp30 ELISA	AgRP Gene Symbol: AgRP Gene ID: 181 Accession #: O00253 ELISA Kit: RayBio Human AgRP ELISA	Amphiregulin Gene Symbol: AREG Gene ID: 374 Accession #: P15514 ELISA Kit: RayBio Human AREG ELISA	Angiogenin Gene Symbol: ANG Gene ID: 283 Accession #: P03950 ELISA Kit: RayBio Human Angiogenin ELISA	Angiopoietin-2 Gene Symbol: ANGPT2 Gene ID: 285 Accession #: O15123 ELISA Kit: RayBio Human ANGPT2 ELISA
Axl Gene Symbol: Axl Gene ID: 558 Accession #: P30530 ELISA Kit: RayBio Human Axl ELISA	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: RayBio Human BDNF ELISA	bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	BLC (CXCL13) Gene Symbol: CXCL13 Gene ID: 10563 Accession #: O43927 ELISA Kit: RayBio Human BLC ELISA	BMP-4 Gene Symbol: BMP4 Gene ID: 652 Accession #: P12644 ELISA Kit: RayBio Human BMP-4 ELISA
BMP-6 Gene Symbol: BMP6 Gene ID: 654 Accession #: P22004 ELISA Kit: RayBio Human BMP-6 ELISA	beta-NGF Gene Symbol: NGF Gene ID: 4803 Accession #: P01138 ELISA Kit: RayBio Human beta-NGF ELISA	Betacellulin (BTC) Gene Symbol: BTC Gene ID: 685 Accession #: P35070 ELISA Kit: RayBio Human BTC ELISA	CCL28 (MEC) Gene Symbol: CCL28 Gene ID: 56477 Accession #: Q9NRJ3 ELISA Kit: RayBio Human CCL28 ELISA	CK beta 8-1 (CCL23) Gene Symbol: CCL23 Gene ID: 6368 Accession #: P55773 ELISA Kit: RayBio Human Ckbeta8 ELISA
CNTF Gene Symbol: CNTF Gene ID: 1270 Accession #: P26441 ELISA Kit: RayBio Human CNTF ELISA	CTACK (CCL27) Gene Symbol: CCL27 Gene ID: 10850 Accession #: Q9Y4X3 ELISA Kit: RayBio Human CTACK ELISA	Dtk Gene Symbol: TYRO3 Gene ID: 7301 Accession #: Q06418 ELISA Kit: RayBio Human Dtk ELISA	EGF Gene Symbol: EGF Gene ID: 1950 Accession #: P01133 ELISA Kit: RayBio Human EGF ELISA	EGFR Gene Symbol: EGFR Gene ID: 1956 Accession #: P00533 ELISA Kit: RayBio Human EGFR ELISA
ENA-78 (CXCL5) Gene Symbol: CXCL5 Gene ID: 6374 Accession #: P42830 ELISA Kit: RayBio Human ENA-78 ELISA	Eotaxin-1 (CCL11) Gene Symbol: CCL11 Gene ID: 6356 Accession #: P51671 ELISA Kit: RayBio Human Eotaxin-1 ELISA	Eotaxin-2 (MPIF-2/CCL24) Gene Symbol: Ccl24 Gene ID: 6369 Accession #: O00175 ELISA Kit: RayBio Human Eotaxin-2 ELISA	Eotaxin-3 (CCL26) Gene Symbol: CCL26 Gene ID: 10344 Accession #: Q9Y258 ELISA Kit: RayBio Human Eotaxin-3 ELISA	Fas (TNFRSF6/Apo-1) Gene Symbol: Fas Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA
FGF-4 Gene Symbol: FGF4 Gene ID: 2249 Accession #: P08620 ELISA Kit: RayBio Human FGF-4 ELISA	FGF-6 Gene Symbol: FGF6 Gene ID: 2251 Accession #: P10767 ELISA Kit: RayBio Human FGF-6 ELISA	FGF-7 (KGF) Gene Symbol: FGF7 Gene ID: 2252 Accession #: P21781 ELISA Kit: RayBio Human FGF-7 ELISA	FGF-9 Gene Symbol: FGF9 Gene ID: 2254 Accession #: P31371 ELISA Kit: RayBio Human FGF-9 ELISA	Flt-3 Ligand Gene Symbol: FLT3LG Gene ID: 2323 Accession #: P49771 ELISA Kit: RayBio Human Flt-3 L ELISA
Fractalkine (CX3CL1) Gene Symbol: CX3CL1 Gene ID: 6376 Accession #: P78423 ELISA Kit: RayBio Human Fractalkine ELISA	GCP-2 (CXCL6) Gene Symbol: CXCL6 Gene ID: 6372 Accession #: P80162 ELISA Kit: RayBio Human GCP-2 ELISA	GCSF Gene Symbol: CSF3 Gene ID: 1440 Accession #: P09919 ELISA Kit: RayBio Human G-CSF ELISA	GDNF Gene Symbol: GDNF Gene ID: 2668 Accession #: P39905 ELISA Kit: RayBio Human GDNF ELISA	GITR (TNFRSF18) Gene Symbol: TNFRSF18 Gene ID: 8784 Accession #: Q9Y5U5 ELISA Kit: RayBio Human GITR ELISA
GITR Ligand (TNFSF18) Gene Symbol: TNFSF18 Gene ID: 8995 Accession #: Q9UNG2 ELISA Kit: RayBio Human GITR L ELISA	GM-CSF Gene Symbol: CSF2 Gene ID: 1437 Accession #: P04141 ELISA Kit: RayBio Human GM-CSF ELISA	GRO alpha/beta/gamma Gene Symbol: CXCL1/CXCL2/CXCL3 Gene ID: 2919/2920/2921 Accession #: P09341/P19875/P19876 ELISA Kit: RayBio	GRO alpha (CXCL1) Gene Symbol: CXCL1 Gene ID: 2919 Accession #: P09341 ELISA Kit: RayBio Human GRO-alpha ELISA	HCC-4 (CCL16) Gene Symbol: CCL16 Gene ID: 6360 Accession #: O15467 ELISA Kit: RayBio Human HCC-4 ELISA
HGF Gene Symbol: HGF Gene ID: 3082 Accession #: P14210 ELISA Kit: RayBio Human HGF ELISA	I-309 (TCA-3/CCL1) Gene Symbol: CCL1 Gene ID: 6346 Accession #: P22362 ELISA Kit: RayBio Human I-309 ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	ICAM-3 (CD50) Gene Symbol: ICAM3 Gene ID: 3385 Accession #: P32942 ELISA Kit: RayBio Human ICAM-3 ELISA	IFN-gamma Gene Symbol: IFNG Gene ID: 3458 Accession #: P01579 ELISA Kit: RayBio Human IFN-gamma ELISA
IGFBP-1 Gene Symbol: IGFBP1 Gene ID: 3484 Accession #: P08833 ELISA Kit: RayBio Human IGFBP-1 ELISA	IGFBP-2 Gene Symbol: IGFBP2 Gene ID: 3485 Accession #: P18065 ELISA Kit: RayBio Human IGFBP-2 ELISA	IGFBP-3 Gene Symbol: IGFBP3 Gene ID: 3486 Accession #: P17936 ELISA Kit: RayBio Human IGFBP-3 ELISA	IGFBP-4 Gene Symbol: IGFBP4 Gene ID: 3487 Accession #: P22692 ELISA Kit: RayBio Human IGFBP-4 ELISA	IGFBP-6 Gene Symbol: IGFBP6 Gene ID: 3489 Accession #: P24592 ELISA Kit: RayBio Human IGFBP-6 ELISA
IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	IGF-1 R Gene Symbol: IGF1R Gene ID: 3480 Accession #: P08069 ELISA Kit: RayBio Human IGF-1 R ELISA	IL-1 R4 (ST2) Gene Symbol: IL1RL1 Gene ID: 9173 Accession #: Q01638 ELISA Kit: RayBio Human IL-1 R4 ELISA	IL-1 R1 Gene Symbol: IL1R1 Gene ID: 3554 Accession #: P14778 ELISA Kit: RayBio Human IL-1 sRI ELISA	IL-10 Gene Symbol: IL10 Gene ID: 3586 Accession #: P22301 ELISA Kit: RayBio Human IL-10 ELISA
IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA	IL-12 p40 Gene Symbol: IL12B Gene ID: 3593 Accession #: P29460 ELISA Kit: RayBio Human IL-12 p40 ELISA	IL-12 p70 Gene Symbol: IL12A Gene ID: 3592 Accession #: P29459 ELISA Kit: RayBio Human IL-12 p70 ELISA	IL-13 Gene Symbol: IL13 Gene ID: 3596 Accession #: P35225 ELISA Kit: RayBio Human IL-13 ELISA	IL-15 Gene Symbol: IL15 Gene ID: 3600 Accession #: P40933 ELISA Kit: RayBio Human IL-15 ELISA
IL-16 Gene Symbol: IL16 Gene ID: 3603 Accession #: Q14005 ELISA Kit: RayBio Human IL-16 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552 ELISA Kit: RayBio Human IL-17A ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA	IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-1 ra (IL-1 F3) Gene Symbol: IL1RN Gene ID: 3557 Accession #: P18510 ELISA Kit: RayBio Human IL-1 RA ELISA
IL-2 Gene Symbol: IL2 Gene ID: 3558 Accession #: P60568 ELISA Kit: RayBio Human IL-2 ELISA	IL-2 R alpha Gene Symbol: IL2RA Gene ID: 3559 Accession #: P01589 ELISA Kit: RayBio Human IL-2 R alpha ELISA	IL-3 Gene Symbol: IL3 Gene ID: 3562 Accession #: P08700 ELISA Kit: RayBio Human IL-3 ELISA	IL-4 Gene Symbol: IL4 Gene ID: 3565 Accession #: P05112 ELISA Kit: RayBio Human IL-4 ELISA	IL-5 Gene Symbol: IL5 Gene ID: 3567 Accession #: P05113 ELISA Kit: RayBio Human IL-5 ELISA
IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-6 R Gene Symbol: IL6R Gene ID: 3570 Accession #: P08887 ELISA Kit: RayBio Human IL-6 sR ELISA	IL-7 Gene Symbol: IL7 Gene ID: 3574 Accession #: P13232 ELISA Kit: RayBio Human IL-7 ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	I-TAC (CXCL11) Gene Symbol: CXCL11 Gene ID: 6373 Accession #: O14625 ELISA Kit: RayBio Human I-TAC ELISA
Leptin Gene Symbol: LEP Gene ID: 3952 Accession #: P41159 ELISA Kit: RayBio Human Leptin ELISA	Light (TNFSF14) Gene Symbol: TNFSF14 Gene ID: 8740 Accession #: O43557 ELISA Kit: RayBio Human LIGHT ELISA	Lymphotactin (XCL1) Gene Symbol: XCL1 Gene ID: 6375 Accession #: P47992 ELISA Kit: RayBio Human Lympotactin ELISA	MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	MCP-2 (CCL8) Gene Symbol: CCL8 Gene ID: 6355 Accession #: P80075 ELISA Kit: RayBio Human MCP-2 ELISA
MCP-3 (MARC/CCL7) Gene Symbol: CCL7 Gene ID: 6354 Accession #: P80098 ELISA Kit: RayBio Human MCP-3 ELISA	MCP-4 (CCL13) Gene Symbol: CCL13 Gene ID: 6357 Accession #: Q99616 ELISA Kit: RayBio Human MCP-4 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	MDC (CCL22) Gene Symbol: CCL22 Gene ID: 6367 Accession #: O00626 ELISA Kit: RayBio Human MDC ELISA	MIF Gene Symbol: MIF Gene ID: 4282 Accession #: P14174 ELISA Kit: RayBio Human MIF ELISA
MIG (CXCL9) Gene Symbol: CXCL9 Gene ID: 4283 Accession #: Q07325 ELISA Kit: RayBio Human MIG ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MIP-1 beta (CCL4) Gene Symbol: CCL4 Gene ID: 6351 Accession #: P13236 ELISA Kit: RayBio Human MIP-1 beta ELISA	MIP-1 delta (CCL15) Gene Symbol: CCL15 Gene ID: 6359 Accession #: Q16663 ELISA Kit: RayBio Human MIP-1 delta ELISA	MIP-3 alpha (CCL20) Gene Symbol: CCL20 Gene ID: 6364 Accession #: P78556 ELISA Kit: RayBio Human MIP-3 alpha ELISA
MIP-3 beta (CCL19) Gene Symbol: CCL19 Gene ID: 6363 Accession #: Q99731 ELISA Kit: RayBio Human MIP-3 beta ELISA	MSP alpha/beta Gene Symbol: MST1 Gene ID: 4485 Accession #: P26927 ELISA Kit: RayBio Human MSP ELISA	NAP-2 (PPBP/CXCL7) Gene Symbol: Ppbp Gene ID: 5473 Accession #: P02775 ELISA Kit: RayBio Human NAP-2 ELISA	NT-3 Gene Symbol: NTF3 Gene ID: 4908 Accession #: P20783 ELISA Kit: RayBio Human NT-3 ELISA	NT-4 Gene Symbol: NTF4 Gene ID: 4909 Accession #: P34130 ELISA Kit: RayBio Human NT-4 ELISA
Oncostatin M Gene Symbol: OSM Gene ID: 5008 Accession #: P13725 ELISA Kit: RayBio Human OSM ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	PARC (CCL18) Gene Symbol: CCL18 Gene ID: 6362 Accession #: P55774 ELISA Kit: RayBio Human PARC ELISA	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	PLGF Gene Symbol: PGF Gene ID: 5228 Accession #: P49763 ELISA Kit: RayBio Human PLGF ELISA
RANTES (CCL5) Gene Symbol: CCL5 Gene ID: 6352 Accession #: P13501 ELISA Kit: RayBio Human RANTES ELISA	SCF Gene Symbol: KITLG Gene ID: 4254 Accession #: P21583 ELISA Kit: RayBio Human SCF ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA	gp130 Gene Symbol: Il6st Gene ID: 3572 Accession #: P40189 ELISA Kit: RayBio Human sgp130 ELISA	TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA
TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA	TARC (CCL17) Gene Symbol: CCL17 Gene ID: 6361 Accession #: Q92583 ELISA Kit: RayBio Human TARC ELISA	TECK (CCL25) Gene Symbol: CCL25 Gene ID: 6370 Accession #: O15444 ELISA Kit: RayBio Human TECK ELISA	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA
Thrombopoietin (TPO) Gene Symbol: THPO Gene ID: 7066 Accession #: P40225 ELISA Kit: RayBio Human TPO ELISA	TIMP-1 Gene Symbol: TIMP1 Gene ID: 7076 Accession #: P01033 ELISA Kit: RayBio Human TIMP-1 ELISA	TIMP-2 Gene Symbol: TIMP2 Gene ID: 7077 Accession #: P16035 ELISA Kit: RayBio Human TIMP-2 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA
TRAIL R3 (TNFRSF10C) Gene Symbol: TNFRSF10C Gene ID: 8794 Accession #: O14798 ELISA Kit: RayBio Human TRAIL R3 ELISA	TRAIL R4 (TNFRSF10D) Gene Symbol: TNFRSF10D Gene ID: 8793 Accession #: Q9UBN6 ELISA Kit: RayBio Human TRAIL R4 ELISA	uPAR Gene Symbol: PLAUR Gene ID: 5329 Accession #: Q03405 ELISA Kit: RayBio Human uPAR ELISA	VEGF-A Gene Symbol: VEGFA Gene ID: 7422 Accession #: P15692 ELISA Kit: RayBio Human VEGF-A ELISA	VEGF-D Gene Symbol: FIGF Gene ID: 2277 Accession #: O43915 ELISA Kit: RayBio Human VEGF-D ELISA

Typical Data

Kit Components

Human Cytokine Antibody Array C1000 Membranes
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated HRP
Detection Buffer C
Detection Buffer D
Lysis Buffer
8-Well Incubation Tray
Plastic Sheets
Array Templates
Manual

Additional Materials Required

Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It^® or EpiChem II Benchtop Darkroom or GE's ImageQuant^™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.

Assay Procedure Summary

Block membranes
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis

Storage/Stability

reliable and excellent cytokine array
Rating
I used these arrays to identified secreted factors from my cultured conditioned media from co-culture of immune cells with prostate cancer cells. The products gave me very consistent results. In addition to identification of the targets, it also allows quantifying the secreted factors from the conditions that I want to compare.
Review by Stancy Liou, from Clark Atlanta University, on Jul 29, 2024
Great product
Rating
Used to probe supernatant from cultured microglia. Blots came out with higher background than expected but otherwise easy to use.
Review by William Antoniades, from University of Georgia, on Mar 1, 2023
Great product
Rating
I used this array for the second time to study the effect of my compound of different cytokine expression. I published the paper recently:Messeha Messeha SS, Zarmouh NO, Mendonca P, Carolyn Cotton, Karam F A Soliman: Molecular mechanism of gossypol mediating CCL2 and IL‑8 attenuation in triple‑negative breast cancer cells. Molecular medicine reports 22: 1213-1226, 2020.
Review by Samia Messeha, from Florida A & M University, Tallahassee, Florida, on Sep 17, 2020
Great product
Rating
Easy, reliable and replicable
Review by Raquel Buj Gomez, from Penn State, on Sep 19, 2019
Great product
Rating
This is a very trustworthy product.
Review by Ruzanna Shkhyan, from University of Southern California, on Sep 10, 2019
Great product
Rating
I have been using these arrays and having pretty good results. My different treatments up and down regulate the expression of these cytokines, which can be clearly seen on the membrane spots.
Review by Patricia Mendonca, from Florida A & M University, on Jun 8, 2018
Great product
Rating
I used this product for testing tumor tissues protein lysates to compare cytokine between control and treated conditions. The results so far has been satisfactory
Review by HIMANSHU ARORA, on Apr 22, 2018

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Target Names

Typical Data

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Assay Procedure Summary

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reliable and excellent cytokine array

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reliable and excellent cytokine array

Great product

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Human Cytokine Array C1000

Human Cytokine Array C1000

Featured Products

Human Cytokine Array C1000

Product Description

Specifications

Product Features

Target Names

Typical Data

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

Featured Products

Human Cytokine Array C1000Human Cytokine Array C1000

Product Description

Specifications

Product Features

Target Names

Typical Data

Kit Components

Additional Materials Required

Assay Procedure Summary

Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

reliable and excellent cytokine array

Great product

Great product

Great product

Great product

Great product

Great product

Ask A Question

reliable and excellent cytokine array

Great product

Great product

Great product

Great product

Great product

Great product

Human Cytokine Array C1000

Human Cytokine Array C1000