Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Human Cytokine Antibody Array C1000 Membranes
Wash Buffer 1
Wash Buffer 2
Biotinylated Detection Antibody Cocktail
Detection Buffer C
Detection Buffer D
8-Well Incubation Tray
Other Materials Required
Pipettors, pipet tips and other common lab consumables
Orbital shaker or oscillating rocker
Tissue Paper, blotting paper or chromatography paper
Adhesive tape or Saran Wrap
Distilled or de-ionized water
A chemiluminescent blot documentation system (such as UVP's ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Incubate with Sample
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with HRP-Conjugated Streptavidin
Incubate with Detection Buffers
Image with chemiluminescent imaging system
Perform densitometry and analysis
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
I used this array for the second time to study the effect of my compound of different cytokine expression. I published the paper recently:
Messeha Messeha SS, Zarmouh NO, Mendonca P, Carolyn Cotton, Karam F A Soliman: Molecular mechanism of gossypol mediating CCL2 and IL‑8 attenuation in triple‑negative breast cancer cells. Molecular medicine reports 22: 1213-1226, 2020.
Samia Messeha Florida A & M University, Tallahassee, Florida09/18/2020, 10:24 AM
I trust the product from RayBiotech. It shows accurate results. I published the following paper using the human cytokine array:
Messeha, S.S., Zarmouh, N.O., Mendonca, P., Alwagdani, H., Kolta, M.G., Soliman, K.F.A., 2018. The inhibitory effects of plumbagin on the NF-B pathway and CCL2 release in racially different triple-negative breast cancer cells. PloS one 13, e0201116.
Samia Messeha Florida A & M University, Tallahassee, Florida09/18/2020, 10:17 AM
Easy, reliable and replicable
Raquel Buj Gomez Penn State09/20/2019, 02:26 PM
This is a very trustworthy product.
Ruzanna Shkhyan University of Southern California09/11/2019, 05:18 PM
I have been using these arrays and having pretty good results. My different treatments up and down regulate the expression of these cytokines, which can be clearly seen on the membrane spots.
Patricia Mendonca Florida A & M University06/08/2018, 09:23 PM
I used this product for testing tumor tissues protein lysates to compare cytokine between control and treated conditions. The results so far has been satisfactory