Human Cytokine Array C2000
RayBio® C-Series Human Cytokine Antibody Array 2000 Kit. A combination of Human Cytokine Antibody Array C6, C7, & C8. Detects 174 Human Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
Size | 2 Sample Kit, 4 Sample Kit, 8 Sample Kit |
---|---|
Species | Human |
Number of Targets Detected | 174 |
Quantitative/Semi-Quantitative | Semi-Quantitative |
Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
Solid Support | Membrane |
Method Of Detection | Chemiluminescence |
Design Principle | Sandwich-based |
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Scroll over each target protein for more information | ||||
---|---|---|---|---|
Adiponectin (ACRP30)
| Activin A
| AgRP
| ALCAM (CD166)
| Amphiregulin
|
Angiogenin
| Angiopoietin-2
| Axl
| CD80 (B7-1)
| BDNF
|
bFGF
| BLC (CXCL13)
| BMP-4
| BMP-5
| BMP-6
|
BMP-7
| beta-NGF
| Betacellulin (BTC)
| Cardiotrophin-1 (CT-1)
| CCL28 (MEC)
|
CD14
| Ck beta 8-1 (CCL23)
| CNTF
| CTACK (CCL27)
| CXCL16
|
DR6 (TNFRSF21)
| Dtk
| EGF
| EGFR
| ENA-78 (CXCL5)
|
Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
| Eotaxin-3 (CCL26)
| ErbB3
|
E-Selectin
| Fas (TNFRSF6/Apo-1)
| Fas Ligand (TNFSF6)
| FGF-4
| FGF-6
|
FGF-7 (KGF)
| FGF-9
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| GCP-2 (CXCL6)
|
GCSF
| GDNF
| GITR (TNFRSF18)
| GITR Ligand (TNFSF18)
| GM-CSF
|
GRO alpha/beta/gamma
| GRO alpha (CXCL1)
| HCC-4 (CCL16)
| HGF
| I-309 (TCA-3/CCL1)
|
ICAM-1 (CD54)
| ICAM-2 (CD102)
| ICAM-3 (CD50)
| IFN-gamma
| IGFBP-1
|
IGFBP-2
| IGFBP-3
| IGFBP-4
| IGFBP-6
| IGF-1
|
IGF-1 R
| IGF-2
| IL-1 R2
| IL-1 R4 (ST2)
| IL-1 R1
|
IL-10
| IL-10 R beta
| IL-11
| IL-12 p40
| IL-12 p70
|
IL-13
| IL-13 R alpha 2
| IL-15
| IL-16
| IL-17A
|
IL-18 BP alpha
| IL-18 R beta (AcPL)
| IL-1 alpha (IL-1 F1)
| IL-1 beta (IL-1 F2)
| IL-1 ra (IL-1 F3)
|
IL-2
| IL-2 R beta (CD122)
| IL-2 R gamma (Common gamma Chain)
| IL-2 R alpha
| IL-21 R
|
IL-3
| IL-4
| IL-5
| IL-5 R alpha
| IL-6
|
IL-6 R
| IL-7
| IL-8 (CXCL8)
| IL-9
| IP-10 (CXCL10)
|
I-TAC (CXCL11)
| LAP/TGF beta 1
| Leptin
| Leptin R
| LIF
|
Light (TNFSF14)
| L-Selectin (CD62L)
| Lymphotactin (XCL1)
| MCP-1 (CCL2)
| MCP-2 (CCL8)
|
MCP-3 (MARC/CCL7)
| MCP-4 (CCL13)
| M-CSF
| M-CSF R
| MDC (CCL22)
|
MIF
| MIG (CXCL9)
| MIP-1 alpha (CCL3)
| MIP-1 beta (CCL4)
| MIP-1 delta (CCL15)
|
MIP-3 alpha (CCL20)
| MIP-3 beta (CCL19)
| MMP-1
| MMP-13
| MMP-3
|
MMP-9
| MPIF-1 (CCL23)
| MSP alpha/beta
| NAP-2 (PPBP/CXCL7)
| NGFR (TNFRSF16)
|
NT-3
| NT-4
| Oncostatin M
| Osteoprotegerin (TNFRSF11B)
| PARC (CCL18)
|
PDGF-AA
| PDGF R alpha
| PDGF R beta
| PDGF-AB
| PDGF-BB
|
PECAM-1 (CD31)
| PLGF
| Prolactin
| RANTES (CCL5)
| SCF
|
SCF R (CD117/c-kit)
| SDF-1 alpha (CXCL12 alpha)
| SDF-1 beta (CXCL12 beta)
| gp130
| Siglec-5 (CD170)
|
TNF RII (TNFRSF1B)
| TNF RI (TNFRSF1A)
| TARC (CCL17)
| TECK (CCL25)
| TGF beta 2
|
TGF alpha
| TGF beta 3
| TGF beta 1
| Thrombopoietin (TPO)
| Tie-1
|
Tie-2
| TIMP-1
| TIMP-2
| TIMP-4
| TNF alpha
|
TNF beta (TNFSF1B)
| TRAIL R3 (TNFRSF10C)
| TRAIL R4 (TNFRSF10D)
| uPAR
| VE-Cadherin (CDH5)
|
VEGF-A
| VEGFR2
| VEGFR3
| VEGF-D
|
Species Detected
Human
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Human Cytokine Antibody Array C2000 Membranes
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated HRP
- Detection Buffer C
- Detection Buffer D
- Lysis Buffer
- 8-Well Incubation Tray
- Plastic Sheets
- Array Templates
- Manual
Other Materials Required
- Pipettors, pipet tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Tissue Paper, blotting paper or chromatography paper
- Adhesive tape or Saran Wrap
- Distilled or de-ionized water
- A chemiluminescent blot documentation system (such as UVP??s ChemiDoc-It® or EpiChem II Benchtop Darkroom or GE's ImageQuant™ LAS 4000 or Amersham Imagers 600 and 680), X-ray Film and a suitable film processor, or another chemiluminescent detection system.
Protocol Outline
- Block membranes
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with HRP-Conjugated Streptavidin
- Incubate with Detection Buffers
- Image with chemiluminescent imaging system
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Ukropec J, Penesova A, Kopkova M, Pura M, Vlcek M,et al.Adipokine Protein Expression Pattern in Growth Hormone Deficiency Predisposes to the Increased Fat Cell Size and the Whole Body Metabolic Derangements. J Clin Endocrinol Metab. 2008;93: 2255-2262
Species:
Human
Sample Type:
Tissue Lysate (tissue Lysates)
Lin, Yu-Ching, et al. "Human umbilical mesenchymal stem cells promote recovery after ischemic stroke." Stroke 42.7 (2011): 2045-2053.
Species:
Rat
Sample Type:
Tissue lysate (cortices injected with HUMSCs)
Ho Ia., Toh H., Ng W., et al. Human bone marrow-derived mesenchymal stem cells suppress human glioma growth through inhibition of angiogenesis. Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247
Species:
Human
Sample Type:
Conditioned Media (Mesenchymal stem cell conditioned media)
Ho I., Toh H., Ng W., et al. Human Bone Marrow?Derived Mesenchymal Stem Cells Suppress Human Glioma Growth Through Inhibition of Angiogenesis, Stem Cells. 2013 Jan;31(1):146-55. doi: 10.1002/stem.1247.
Species:
Human
Sample Type:
Conditioned Media (glioma cells and mesenchymal stem cells )
Tsai PJ, Yeh CC, Huang WJ, Min MY, Huang TH, Ko TL, Huang PY, Chen TH, Hsu SPC, Soong BW, Fu YS. Xenografting of human umbilical mesenchymal stem cells from Wharton's jelly ameliorates mouse spinocerebellar ataxia type 1. Transl Neurodegener. 2019 Sep 5;8:29. doi: 10.1186/s40035-019-0166-8. PMID: 31508229; PMCID: PMC6727337.
Species:
Human
Sample Type:
Conditioned Media
Perez-Garcia JL., et al. Identification of TNF-a and MMP-9 as potential baseline predictive serum markers of sunitinib activity in patients with renal cell carcinoma using a human cytokine array. British Journal of Cancer (2009) 101, 1876 - 1883
Species:
Human
Sample Type:
Serum
Kim N, Dabrowska A, Jenner RG, Aldovini A. Human and Simian Immunodeficiency Virus-Mediated Upregulation of the Apoptotic Factor TRAIL Occurs in Antigen-Presenting Cells from AIDS-Susceptible but Not from AIDS-Resistant Species. J Virol. 2007; 81(14):7584-7597.
Species:
Non-Human Primate
Sample Type:
Conditioned Media (HIV-infected peripheral blood mononuclear cell (PBMC) conditioned media, Chimp)
Kim N, Dabrowska A, Jenner RG, Aldovini A. Human and Simian Immunodeficiency Virus-Mediated Upregulation of the Apoptotic Factor TRAIL Occurs in Antigen-Presenting Cells from AIDS-Susceptible but Not from AIDS-Resistant Species. J Virol. 2007; 81(14):7584-7597.
Species:
Human
Sample Type:
Conditioned Media (HIV-infected peripheral blood mononuclear cell (PBMC) conditioned media)
Anderson J., et al. Phosphoproteomic Profiling Reveals IL6-mediated Paracrine Signaling within the Ewing Sarcoma Family of Tumors. Mol Cancer Res., Published Online First August 4, 2014; doi: 10.1158/1541-7786.MCR-14-0159
Species:
Human
Sample Type:
Conditioned Media (Serum free media from A673 stimulated cells)
Poffet, F., et al. "Autoimmune progesterone dermatitis: potential role of cutaneous angiogenin expression?." Dermatology 223.1 (2011): 32-35.
Species:
Human
Sample Type:
Tissue Lysate (skin biopsy)
- Great productUse C-2000 Human cytokine array for culture cell condition media (media from complete serum-starved cancer cells) for multiple cytokine releases during drug treatments. Overall, membranes from Raybiotech turn out very well and are able to capture multiple condition media release cytokines and confirm some cytokines results with ELISA.
from University of Kentucky,
on
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