Name: Human Phospho-EGFR (Activated) Cell-Based ELISA Kit
SKU: CBEL-EGFR-1
Availability: InStock

Product Description

Specifications

Size	1 Plate Kit, 2 Plate Kit, 5 Plate Kit
Estimated Lead Time	1-2 business days
Species	Human
Specificity	The antibodies provided in this kit recognizes human Tyrosine-phosphorylated-EGFR and total EGFR for comparison.
Compatible Sample Types	Adherent Cell Culture
Accession Number	P00533
Gene Id	1956
Gene Symbols	EGFR, ERBB, ERBB1, HER1
Protein Name / Synonyms	Epidermal growth factor receptor (EC 2.7.10.1) (Proto-oncogene c-ErbB-1) (Receptor tyrosine-protein kinase erbB-1)
Quantitative/Semi-Quantitative	Semi-Quantitative
Solid Support	96-well Microplate
Design Principle	Cell-based
Method Of Detection	Colorimetric
Research Area	Cardiovascular Disease, Cancer, Post-Translational Modifications, Inflammation, Phosphorylation, HER/ErbB Signaling
Shipping Type	Blue ice
Storage	-20°C

Product Features

Site and signal pathway-specific
In vitro detection of adherent cell culture
No sample lysis is needed
Compatible with a standard ELISA plate reader
Faster results than with ELISA
Adaptable for high-throughput screening and drug discovery

Application Notes

Kit Components

uncoated 96-well Microplate
Wash Buffer A
Wash Buffer B
Fixing Solution
Quenching Buffer
Blocking Buffer
Anti-phospho antibody
Anti-pan antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate
Stop Solution

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacture's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37 ^°C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

Product Features

Site and signal pathway-specific
In vitro detection of adherent cell culture
No sample lysis is needed
Compatible with a standard ELISA plate reader
Faster results than with ELISA
Adaptable for high-throughput screening and drug discovery

Application Notes

Kit Components

uncoated 96-well Microplate
Wash Buffer A
Wash Buffer B
Fixing Solution
Quenching Buffer
Blocking Buffer
Anti-phospho antibody
Anti-pan antibody
HRP-Conjugated Secondary Antibody
TMB One-Step Substrate
Stop Solution

Other Materials Required

Distilled or deionized water
100 ml and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 µl to 1 ml volumes
Adjustable 1-25 ml pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Protocol Outline

Seed 10,000-30,000 cells into each well and incubate overnight.
Apply various treatment, inhibitors or activators according to manufacture's instructions.
Add 100 µl of Fixing Solution into each well and incubate for 20 min at RT with shaking.
Add 200 µl of prepared 1X Quenching Buffer and incubate 20 min at RT.
Add 200 µl of Blocking Solution and incubate for 1 h at 37 ^°C.
Add 50 µl of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
Add 50 µl of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
Add 100 µl of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 µl of Stop Solution to each well.
Read at 450 nm immediately.

Storage/Stability

The entire kit may be stored at -20^°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.

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Product Description

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Product Features

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Storage/Stability

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Featured Products

Human Phospho-EGFR (Activated) Cell-Based ELISA Kit

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Storage/Stability

Documents

Have this product in hand?

Citations

Reviews

Ask A Question

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