Introduction
Protein lactylation plays an unusually prominent role in cell signaling, development, and growth. The RayBio Human Protein Lactylation Antibody Array 1 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple lactylation events on proteins. It can also be used to monitor the activation or function of important biological pathways.
RayBiotech is committed to developing a series of lactylation antibody arrays. The RayBio Human Protein Lactylation Antibody Array 1 is specifically designed for the simultaneous identification of the relative levels of lysine lactylation in 507 different human proteins in cell lysate. By monitoring changes in protein lactylation in your experimental model system, you can verify pathway activation in your cell lines without the need for extensive time and effort required for immunoprecipitation and/or Western Blot analysis.
To use the RayBio Human Protein Lactylation Antibody Array 1, treated or untreated cell lysate is added to the antibody array glass slide wells. The antibody array slide wells are then washed, and a biotinylated anti-Lactyllysine antibody is used to detect the lactylated lysine residues on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can be imaged using a laser scanner, such as the Axon GenePix, in the Cy3 channel.
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µL per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
6Ckine, Activin A, Activin B, Activin C, Activin RIA (ALK-2), Activin RIB (ALK-4), Activin RII A/B, Activin RIIA, Adiponectin (Acrp30), AgRP, ALCAM, Angiogenin, Angiopoietin-1, Angiopoietin-2, Angiopoietin-4, Angiopoietin-like 1, Angiopoietin-like 2, Angiopoietin-like Factor, Angiostatin, APJ, APRIL, AR (Amphiregulin), Artemin, Axl, B7-1 (CD80), BAFF R (TNFRSF13C), BCMA (TNFRSF17), BD-1, BDNF, beta-Catenin, BAX, beta-NGF, BIK, BLC (BCA-1/CXCL13), BMP-2, BMP-3, BMP-3b (GDF-10), BMP-4, BMP-5, BMP-6, BMP-7, BMP-8, BMP-15, BMPR-IA (ALK-3), BMPR-IB (ALK-6), BMPR-II, BTC, Cardiotrophin-1 (CT-1), CCL14 (HCC-1/HCC-3), CCL28 (VIC), CCR1, CCR2, CCR3, CCR4, CCR5, CCR6, CCR7, CCR8, CCR9, CD14, CD27 (TNFRSF7), CD30 (TNFRSF8), CD30 Ligand (TNFSF8), CD40 (TNFRSF5), CD40 Ligand (TNFSF5/CD154), CD 163, Cerberus 1, Chem R23, Chordin-Like 1, Chordin-Like 2, CLC, CNTF, CNTF R alpha, Coagulation Factor III (Tissue Factor), CRIM 1, Cripto-1, CRTH-2, Cryptic, Csk, CTACK (CCL27), CTGF (CCN2), CTLA-4 (CD152), CV-2 (Crossveinless-2), CXCL14 (BRAK), CXCL16, CXCR1 (IL-8 RA), CXCR2 (IL-8 RB), CXCR3, CXCR4 (fusin), CXCR5 (BLR-1), CXCR6, D6, DAN, DANCE, DcR3 (TNFRSF6B), Decorin, Dkk-1, Dkk-3, Dkk-4, DR3 (TNFRSF25), DR6 (TNFRSF21), Dtk, EDA-A2, EDAR, EDG-1, EGF, EGF R (ErbB1), EG-VEGF (PK1), EMAP-II, ENA-78, Endocan, Endoglin (CD105), Endostatin, Endothelin, EN-RAGE, Eotaxin (CCL11), Eotaxin-2 (MPIF-2), Eotaxin-3 (CCL26), Epiregulin, ErbB2, ErbB3, ErbB4, Erythropoietin, E-Selectin, FADD, FAM3B, Fas (TNFRSF6), Fas Ligand, FGF Basic, FGF-BP, FGF R3, FGF R4, FGF R5, FGF-4, FGF-5, FGF-6, FGF-7 (KGF), FGF-8, FGF-9, FGF-10 (KGF-2), FGF-11, FGF-12, FGF-13 1B, FGF-16, FGF-17, FGF-18, FGF-19, FGF-20, FGF-21, FGF-23, FLRG, Flt-3 Ligand, Follistatin, Follistatin-like 1, Fractalkine, Frizzled-1, Frizzled-3, Frizzled-4, Frizzled-5, Frizzled-6, Frizzled-7, Galectin-3, GASP-1 (WFIKKNRP), GASP-2 (WFIKKN), GCP-2 (CXCL6), GCSF, G-CSF R (CD 114), GDF1, GDF3, GDF5, GDF8, GDF9, GDF11, GDF-15, GDNF, GFR alpha-1, GFR alpha-2, GFR alpha-3, GFR alpha-4, GITR (TNFRF18), GITR Ligand (TNFSF18), Glucagon, Glut1, Glut2, Glut3, Glut5, Glypican 3, Glypican 5, GM-CSF, GM-CSF R alpha, Granzyme A, GREMLIN, GRO, GRO-a, Growth Hormone (GH), Growth Hormone R (GHR), HB-EGF, HCC-4 (CCL16), HCR (CRAM-A/B), Hepassocin, GLO-1, HGF, HGFR, HRG-alpha, HRG-beta 1, HVEM (TNFRSF14), I-309, ICAM-1, ICAM-2, ICAM-3 (CD50), ICAM-5, IFN-alpha/beta R1, IFN-alpha/beta R2, IFN-beta, IFN-gamma, IFN-gamma R1, IGFBP-1, IGFBP-2, IGFBP-3, IGFBP-4, IGFBP-6, IGFBP-rp1 (IGFBP-7), IGF-I, IGF-I SR, IGF-II, IGF-II R, IL-1 alpha, IL-1 beta, IL-1 F5 (FIL1delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R3 (IL-1 R AcP), IL-1 R4 (ST2), IL-1 R6 (IL-1 Rrp2), IL-1 R8, IL-1 R9, IL-1 ra, IL-1 sRI, IL-1 sRII, IL-2, IL-2 R alpha, IL-2 R beta (CD122), IL-2 R gamma, IL-3, IL-3 R alpha, IL-4, IL-4 R, IL-5, IL-5 R alpha, IL-6, IL-6 R, IL-7, IL-7 R alpha, IL-8, IL-9, IL-10, IL-10 R alpha, IL-10 R beta, IL-11, IL-12 p40, IL-12 p70, IL-12 R beta 1, IL-12 R beta 2, IL-13, IL-13 R alpha 1, IL-13 R alpha 2, IL-15, IL-15 R alpha, IL-16, IL-17, IL-17B, IL-17B R, IL-17C, IL-17D, IL-17E, IL-17F, IL-17R, IL-17RC, IL-17RD, IL-18 BPa, IL-18 R alpha (IL-1 R5), IL-18 R beta (AcPL), IL-19, IL-20, IL-20 R alpha, IL-20 R beta, IL-21, IL-21 R, IL-22, IL-22 BP, IL-22 R, IL-23, IL-23 R, IL-24, IL-26, IL-27, IL-28A, IL-29, IL-31, IL-31 RA, BACE-1, FACX, Insulin, Insulin R, Insulysin (IDE), IP-10, I-TAC (CXCL11), Kininostatin (kininogen), Kremen-1, Kremen-2, Latent TGF-beta bp1, LBP, Lck, LECT2, Lefty - A, Leptin (OB), Leptin R, LFA-1 alpha, LIF, LIF R alpha, LIGHT (TNFSF14), Lipocalin-1, Lipocalin-2, LRP-1, LRP-6, L-Selectin (CD62L), Lymphotactin (XCL1), Lymphotoxin beta (TNFSF3), Lymphotoxin beta R (TNFRSF3), MAC-1, MCP-1, MCP-2, MCP-3, MCP-4 (CCL13), M-CSF, M-CSF R, MDC, MFG-E8, MFRP, MICA, MIF, MIG, MIP-1a, MIP-1b, MIP-1d, MIP 2, MIP-3 alpha, MIP-3 beta, MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11 (Stromelysin-3), MMP-12, MMP-13, MMP-14, MMP-15, MMP-16 (MT3-MMP), MMP-19, MMP-20, MMP-24 (MT5-MMP), MMP-25 (MT6-MMP), MSP alpha Chain, Musk, NAP-2, NCAM-1 (CD56), Neuritin, NeuroD1, Neuropilin-2, Neurturin, NGF R, Nidgen-1, NOV (CCN3), NrCAM, NRG1 Isoform GGF2, NRG2, NRG3, NT-3, NT-4, Orexin A, Orexin B, OSM, Osteoactivin (GPNMB), Osteocrin, Osteoprotegerin (TNFRSF11B), OX40 Ligand (TNFSF4), PARC (CCL18), PD-ECGF, PDGF R alpha, PDGF R beta, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-C, PDGF-D, PECAM-1 (CD31), Pentraxin3 (TSG-14), Persephin, PF4 (CXCL4), PlGF, PLUNC, Pref-1, Progranulin, Prolactin, P-selectin, RAGE, RANK (TNFRSF11A), RANTES, RELM beta, RELT (TNFRSF19L), ROBO4, S100 A8/A9, S100A10, SAA, SCF, SCF R (CD117), SDF-1 (CXCL12), sFRP-1, sFRP-3, sFRP-4, sgp130, SIGIRR, Siglec-5 (CD170), Siglec-9, SLPI, Smad 1, Smad 4, Smad 5, Smad 7, Smad 8, Prdx6, Soggy-1, Sonic Hedgehog (Shh N-terminal), SPARC, Spinesin, TACI (TNFRSF13B), Tarc, TCCR (WSX-1), TECK (CCL25), TFPI, TGF-alpha, TGF-beta 1, TGF-beta 2, TGF-beta 3, TGF-beta 5, TGF-beta RI (ALK-5), TGF-beta RII, Grb2, TGF-beta RIII, Thrombopoietin (TPO), TPX, Thrombospondin-1, Thrombospondin-2, Thrombospondin-4, Thymopoietin, Tie-1, Tie-2, TIMP-1, TIMP-2, TIMP-3, TIMP-4, TL1A (TNFSF15), TLR1, TLR2, TLR3, TLR4, TMEFF1 (Tomoregulin-1), TMEFF2, TNF-alpha, TNF-beta, TNF RI (TNFRSF1A), TNF RII (TNFRSF1B), TRADD, TRAIL (TNFSF10), TRAIL R1 (DR4/TNFRSF10A), TRAIL R2 (DR5/TNFRSF10B), TRAIL R3 (TNFRSF10C), TRAIL R4 (TNFRSF10D), TRANCE, TREM-1, TROY (TNFRSF19), TSG-6, TSLP R, TWEAK (TNFSF12), TWEAK R (TNFRSF12), Ubiquitin+1, uPA, uPAR, Vasorin, VCAM-1 (CD106), VE-Cadherin, VEGF, VEGF R2 (KDR), VEGF R3, VEGF-B, VEGF-C, VEGF-D, VEGI (TNFSF15), WIF-1, WISP-1 (CCN4), XEDAR
Kit Components
- Human Antibody Array Slide(s)
- Blocking Buffer
- Biotinylated Detection Antibody
- Fluorescent Dye-Conjugated Streptavidin
- Wash Buffer I
- Wash Buffer II
- Wash Buffer III
- Lysis Buffer
- Protease Inhibitor Cocktail
- Adhesive film
Additional Materials Required
- Shaker
- Laser scanner for fluorescence detection
- Aluminum foil
- Distilled water
- Plastic box
- 50 mL Centrifuge tube
- Isopropanol (2-propanol)
Assay Procedure Summary
- Dry the glass slide
- Add Blocking Buffer to each well and incubate.
- Add the sample to each well and incubate.
- Wash the wells.
- Add Biotinylated Detection Antibody solution to each well and incubate.
- Add Fluorescent Dye-Conjugated Streptavidin solution to each well and incubate.
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
Store kit at ≤ -20°C immediately upon arrival. Kit must be used within the 6 months expiration date.
