Protein Lactylation plays an unusually prominent role in cell signaling, development and growth. The RayBio^® G-Series Human Protein Lactylation Antibody Array 8000 is a very rapid, convenient, and sensitive assay that can simultaneously detect multiple protein Lactylation of lysine and be used to monitor the activation or function of important biological pathways.

RayBiotech is committed to developing a series of Lactylation antibody arrays. RayBio^® Human Protein Lactylation Antibody Array 8000 is specifically designed for simultaneous identification of the relative levels of Lactylation of lysine of 8000 different Human Proteins in cell lysate. By monitoring the changes in protein Lactylation in your experimental model system, you can verify pathway activation in your cell lines without spending excess time and effort performing an analysis of immunoprecipitation and/or Western Blot.

To use the RayBio^® G-Series Human Protein Lactylation Antibody Array 8000, treated or untreated cell lysate is added into antibody array glass slide wells. The antibody array slide wells are washed, and a biotinylated anti-Lactyllysine antibody is then used to detect the lactylated-lysine on target proteins. After incubation with a fluorescent dye-conjugated streptavidin (Cy3 equivalent), the slides can then be imaged using a laser scanner, such as the Axon GenePix, using the Cy3 channel.

• High sensitivity and specificity
• Low sample volume (10-100 µL per array)
• Large dynamic range of detection
• Compatible with most sample types
• Test 4 or 8 samples on each slide
• Suitable for high-throughput assays

• Human Antibody Array Slide(s)
• Blocking Buffer
• Biotinylated Detection Antibody
• Fluorescent Dye-Conjugated Streptavidin
• Wash Buffer I
• Wash Buffer II
• Wash Buffer III
• Lysis Buffer
• Protease Inhibitor Cocktail
• Adhesive film

• Shaker
• Laser scanner for fluorescence detection
• Aluminum foil
• Distilled water
• Plastic box
• 50 mL Centrifuge tube
• Isopropanol (2-propanol)

1. Dry the glass slide
2. Add Blocking Buffer to each well and incubate.
3. Add the sample to each well and incubate.
4. Wash the wells.
5. Add Biotinylated Detection Antibody solution to each well and incubate.
6. Add Fluorescent Dye-Conjugated Streptavidin solution to each well and incubate.
7. Scan with a gene microarray laser scanner
8. Perform densitometry and analysis