Monkeypox Virus Proteins

Monkeypox Virus (MPXV) is an enveloped double-stranded DNA virus that belongs to the genus Orthopoxvirus in the family Poxviridae. While monkeypox was first discovered in the colonies of monkey kept for research in 1958, first human case of monkeypox was registered in 1970. Monkeypox is thought to be endemic to West Africa and Central Africa. In 2003, there was a monkeypox outbreak outside of Africa and it occurred in the United States of America. Recently, monkeypox cases have been reported in multiple non-endemic countries raising awareness.

The 196,858-bp monkeypox virus genome consists of ~200 open reading frames with ≥60 amino acid residues. RayBiotech now offers 15 bacterial- or mammalian-derived monkeypox proteins. Recombinant proteins are key components in current infectious disease research. They are widely used for antibody and vaccine development and as key components in immunodiagnostic assays.

---

SDS-PAGE of Recombinant Monkeypox Virus Cell Surface-binding Protein, E8L

Figure 1. Deglycosylation analysis of purified recombinant proteins. The same amount of purified proteins were untreated (Lane 2) or treated with protein deglycosylation enzymes under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one reduced band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.