Product Description

Specifications

Size	48 tests, 96 tests, 192 tests, 480 tests
Estimated Lead Time	1-2 business days
Species	Human
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	13
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Research Area	Necrosis
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
Bcl-2 Gene Symbol: BCL2 Gene ID: 596 Accession #: P10415 ELISA Kit: RayBio Human Bcl-2 ELISA	BID Gene Symbol: BID Gene ID: 637 Accession #: P55957 ELISA Kit: RayBio Human BID ELISA	Caspase-3 Gene Symbol: CASP3 CPP32 Gene ID: 836 Accession #: P42574 ELISA Kit: RayBio	CD40 (TNFRSF5) Gene Symbol: CD40 TNFRSF5 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG CD40L TNFSF5 TRAP Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA
Fas (TNFRSF6/Apo-1) Gene Symbol: FAS APT1 FAS1 TNFRSF6 Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG APT1LG1 CD95L FASL TNFSF6 Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA TNFB TNFSF1 Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A TNFAR TNFR1 Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA
TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B TNFBR TNFR2 Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	TRAIL R1 (TNFRSF10A/DR4) Gene Symbol: TNFRSF10A APO2 DR4 TRAILR1 Gene ID: 8797 Accession #: O00220 ELISA Kit: RayBio Human TRAIL R1 ELISA	TRAIL R2 (TNFRSF10B/DR5) Gene Symbol: TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 UNQ160/PRO186 Gene ID: 8795 Accession #: O14763 ELISA Kit: RayBio Human TRAIL R2 ELISA

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, all components should be stored at 4°C. The Lyophilized Protein Standard Mix (Item 4) should be stored at 4°C or below. Use reagents immediately after preparation. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Product Features

Less sample, more data: up to 25 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
Bcl-2 Gene Symbol: BCL2 Gene ID: 596 Accession #: P10415 ELISA Kit: RayBio Human Bcl-2 ELISA	BID Gene Symbol: BID Gene ID: 637 Accession #: P55957 ELISA Kit: RayBio Human BID ELISA	Caspase-3 Gene Symbol: CASP3 CPP32 Gene ID: 836 Accession #: P42574 ELISA Kit: RayBio	CD40 (TNFRSF5) Gene Symbol: CD40 TNFRSF5 Gene ID: 958 Accession #: P25942 ELISA Kit: RayBio Human CD40 ELISA	CD40 Ligand (TNFSF5) Gene Symbol: CD40LG CD40L TNFSF5 TRAP Gene ID: 959 Accession #: P29965 ELISA Kit: RayBio Human CD40L ELISA
Fas (TNFRSF6/Apo-1) Gene Symbol: FAS APT1 FAS1 TNFRSF6 Gene ID: 355 Accession #: P25445 ELISA Kit: RayBio Human Fas ELISA	Fas Ligand (TNFSF6) Gene Symbol: FASLG APT1LG1 CD95L FASL TNFSF6 Gene ID: 356 Accession #: P48023 ELISA Kit: RayBio Human FasL ELISA	TNF alpha Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	TNF beta (TNFSF1B) Gene Symbol: LTA TNFB TNFSF1 Gene ID: 4049 Accession #: P01374 ELISA Kit: RayBio Human TNF-beta ELISA	TNF RI (TNFRSF1A) Gene Symbol: TNFRSF1A TNFAR TNFR1 Gene ID: 7132 Accession #: P19438 ELISA Kit: RayBio Human sTNFRI ELISA
TNF RII (TNFRSF1B) Gene Symbol: TNFRSF1B TNFBR TNFR2 Gene ID: 7133 Accession #: P20333 ELISA Kit: RayBio Human sTNFRII ELISA	TRAIL R1 (TNFRSF10A/DR4) Gene Symbol: TNFRSF10A APO2 DR4 TRAILR1 Gene ID: 8797 Accession #: O00220 ELISA Kit: RayBio Human TRAIL R1 ELISA	TRAIL R2 (TNFRSF10B/DR5) Gene Symbol: TNFRSF10B DR5 KILLER TRAILR2 TRICK2 ZTNFR9 UNQ160/PRO186 Gene ID: 8795 Accession #: O14763 ELISA Kit: RayBio Human TRAIL R2 ELISA

Application Notes

Suggested Application

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
V-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
If using the Filter 96-Well Microplate (Item 11): Vacuum manifold compatible with a 96-well microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

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