Product Description

Specifications

Size	48 tests, 96 tests, 192 tests, 480 tests
Estimated Lead Time	1-2 business days
Species	Human
Specificity	No detectable or significant cross reactivity was detected between antibodies or targets.
Compatible Sample Types	Cell Culture Supernatants, Plasma, Serum
Quantitative/Semi-Quantitative	Quantitative
Number of Targets Detected	11
Solid Support	Fluorescent Microbeads
Design Principle	Sandwich-based
Method Of Detection	Flow Cytometry
Research Area	Inflammation, Alzheimer's Disease, Parkinson's Disease
Shipping Type	Blue ice
Storage	Mixed storage. See datasheet.

Product Features

Less sample, more data: up to 11 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
MCP-1 Gene Symbol: CCL2 MCP1 SCYA2 Gene ID: 6347 Accession #: P13500 ELISA Kit: Human MCP-1 ELISA Kit	IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: Human IL-6 ELISA Kit	βNGF Gene Symbol: NGF NGFB Gene ID: 4803 Accession #: P01138 ELISA Kit: Human beta-NGF ELISA Kit	TNFα Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: Human TNF alpha ELISA Kit	Fractalkine Gene Symbol: CX3CL1 FKN NTT SCYD1 A-152E5.2 Gene ID: 6376 Accession #: P78423 ELISA Kit: Human Fractalkine ELISA Kit
TREM-2 Gene Symbol: TREM2 Gene ID: 54209 Accession #: Q9NZC2 ELISA Kit: Human TREM-2 ELISA Kit	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: Human BDNF ELISA Kit	TGFβ1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137 ELISA Kit: Human TGF-beta 1 ELISA Kit	VEGF Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692 ELISA Kit: Human VEGF-A ELISA Kit	IL-18 Gene Symbol: IL18 IGIF IL1F4 Gene ID: 3606 Accession #: Q14116 ELISA Kit: Human IL-18 ELISA Kit
RAGE Gene Symbol: MOK RAGE RAGE1 Gene ID: 5891 Accession #: Q9UQ07 ELISA Kit: Human RAGE ELISA Kit

Application Notes

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
Flat-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

Storage/Stability

Upon receipt, the Lyophilized Protein Standard Mix should be stored at -20°C or below. Other components should be stored at 4°C. Use reagents immediately after preparation. Additional vials of the Lyophilized Protein Standard Mix can be purchased separately. If stored at the proper temperature, the kit will retain complete activity for at least 6 months.

Product Features

Less sample, more data: up to 11 proteins can be quantified from only 25 µl or less of your precious sample
4 hour processing time
5-10x more cost-effective than ELISA
Compatible with most flow cytometers
No specialized or dedicated instrument(s) needed
High specificity and system reproducibility
Well-suited for high throughput assays

Target Names

Scroll over each target protein for more information
MCP-1 Gene Symbol: CCL2 MCP1 SCYA2 Gene ID: 6347 Accession #: P13500 ELISA Kit: Human MCP-1 ELISA Kit	IL-6 Gene Symbol: IL6 IFNB2 Gene ID: 3569 Accession #: P05231 ELISA Kit: Human IL-6 ELISA Kit	βNGF Gene Symbol: NGF NGFB Gene ID: 4803 Accession #: P01138 ELISA Kit: Human beta-NGF ELISA Kit	TNFα Gene Symbol: TNF TNFA TNFSF2 Gene ID: 7124 Accession #: P01375 ELISA Kit: Human TNF alpha ELISA Kit	Fractalkine Gene Symbol: CX3CL1 FKN NTT SCYD1 A-152E5.2 Gene ID: 6376 Accession #: P78423 ELISA Kit: Human Fractalkine ELISA Kit
TREM-2 Gene Symbol: TREM2 Gene ID: 54209 Accession #: Q9NZC2 ELISA Kit: Human TREM-2 ELISA Kit	BDNF Gene Symbol: BDNF Gene ID: 627 Accession #: P23560 ELISA Kit: Human BDNF ELISA Kit	TGFβ1 Gene Symbol: TGFB1 TGFB Gene ID: 7040 Accession #: P01137 ELISA Kit: Human TGF-beta 1 ELISA Kit	VEGF Gene Symbol: VEGFA VEGF Gene ID: 7422 Accession #: P15692 ELISA Kit: Human VEGF-A ELISA Kit	IL-18 Gene Symbol: IL18 IGIF IL1F4 Gene ID: 3606 Accession #: Q14116 ELISA Kit: Human IL-18 ELISA Kit
RAGE Gene Symbol: MOK RAGE RAGE1 Gene ID: 5891 Accession #: Q9UQ07 ELISA Kit: Human RAGE ELISA Kit

Application Notes

Kit Components

RayPlex™ Multiplex Bead Cocktail
1X Assay Diluent
20X Wash Buffer
RayBio^® Serum/Plasma Diluent
Lyophilized Protein Standard Mix
1X Biotinylated Detection Antibody Cocktail
10X PE Conjugate
Flow Cytometer Setup Bead Cocktail
Flat-bottom 96-well Microplate
Filter 96-well Microplate

Other Materials Required

Orbital 96-well plate shaker (with ability to reach 1000 rpm)
Flow cytometer with blue and red lasers capable of measuring phycoerythrin (PE) and allophycocyanin (APC) channels
Aluminum foil or 96-well Plate Aluminum Sealers
Distilled water
1.5 ml polypropylene microcentrifuge tubes or similar
Microcentrifuge
Recommended: Vacuum manifold compatible with a 96-well microplate if using the Filter 96-Well Microplate
Optional: 96-well round bottom plate (to prepare samples)
Optional: High throughput sampler (HTS) for 96-well plate reading

Don't have a compatible flow cytometer? RayBiotech provides full testing services for RayPlex™ bead arrays. Get a quote!

Protocol Outline

Prepare Reagents, Standards, and Samples
Add RayPlex™ Multiplex Bead Cocktail to each well
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluorophore
Analyze with a compatible flow cytometer
Perform data analysis

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RayPlex^® Human Neuroinflammation Bead Array (11-Plex)

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Storage/Stability

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Product Description

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Product Features

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RayPlex^® Human Neuroinflammation Bead Array (11-Plex)

RayPlex^® Human Neuroinflammation Bead Array (11-Plex)

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RayPlex® Human Neuroinflammation Bead Array (11-Plex)

Product Description

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Product Features

Target Names

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Storage/Stability

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Featured Products

RayPlex® Human Neuroinflammation Bead Array (11-Plex)

Product Description

Specifications

Product Features

Target Names

Application Notes

Storage/Stability

Documents

Have this product in hand?

Reviews

Ask A Question

RayPlex® Human Neuroinflammation Bead Array (11-Plex)

RayPlex® Human Neuroinflammation Bead Array (11-Plex)

RayPlex^® Human Neuroinflammation Bead Array (11-Plex)

RayPlex^® Human Neuroinflammation Bead Array (11-Plex)

RayPlex^® Human Neuroinflammation Bead Array (11-Plex)

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