Recombinant Human Angiotensin-converting Enzyme 2 (ACE2) with C-terminal His-tag, derived from the transfected human HEK293 cells.
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Product Description
Specifications
| Size | 100 µg, 500 µg, 1000 µg |
| Estimated Lead Time | 1-2 business days |
| Species | Human |
| Accession Number | Q9BYF1 |
| Protein Name / Synonyms | ACE2, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, Metalloprotease MPROT15 |
| Expressed Region | Gln18-Ser740 (Extracellular domain) |
| Expression System | HEK293 cells |
| Tag | C-terminal his-tag |
| Purity | >95 % |
| Purity Determined By | SDS-PAGE under reducing conditions and visualized by Coomassie blue staining |
| Molecular Weight (kDa) | Recombinant protein product has a calculated molecular mass of ∼83 kDa. Due to the abundant glycosylation, it migrates as approximately 90 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below. |
| Format | Liquid |
| Formulation | Supplied as a 0.2 µm filtered solution in PBS (pH 7.4) |
| Concentration (lot specific) | Lot specific (see the label on the vial), determined by BCA protein assay |
| Research Area | Angiogenesis, Inflammation, Antibody Drug Development, Drug Antibody (DA) Detection, Protein Therapeutics, Receptor-Ligand Binding |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Angiotensin-converting enzyme 2 is a membrane-bound enzyme primarily expressed in the lungs, heart, kidneys, intestines, and vascular endothelial cells. It plays a crucial role in regulating blood pressure and fluid balance by converting angiotensin II, a vasoconstrictor, into angiotensin (1-7), which has vasodilatory and anti-inflammatory properties. In addition to its role in cardiovascular homeostasis, angiotensin-converting enzyme 2 serves as the entry receptor for SARS-CoV and SARS-CoV-2, allowing viral attachment and cell entry, making it a key focus in infectious disease research.
Reference: https://pubmed.ncbi.nlm.nih.gov/34867819/
SDS-PAGE Image
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions
Binding Function
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4°C. For long term storage, it is recommended to store at -20°C or -80°C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
Custom Labeling Request
We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.
