Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Purity:
>90 %
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Lateral flow, indirect ELISA, sandwich ELISA, glycosylation analysis, binding assay, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, and vaccine development. Sandwich ELISA: this protein functions as a standard in conjunction with 130-10815 (capture antibody) and 130-10814 (detection antibody).
Source
Species:
SARS-CoV-2
Accession Number:
QHD43416
Expressed Region:
Arg319-Phe541
Protein Name & Synonyms:
Spike protein, S Protein, S2 Subunit
Preparation
Expression System:
Human Embryonic Kidney 293 (HEK293) Cells
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
SDS-PAGE Image
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated. Lane 1: Protein standard ladder (kDa) Lane 2: Untreated protein under reducing conditions Lane 3: Treated protein with deglycosylation enzymes under native conditions Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Binding Function
The product S1 RBD (Cat. 230-30162) was coated in 96 well plate and incubated with the serial diluted human ACE2 protein (Raybio, Cat. 230-30165). The bound ACE2 was detected by anti-ACE2 antibody using ELISA. The calculated EC50 is 4.23-9.26 ng/mL.
Stability & Storage
Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.
