Recombinant SARS-CoV-2, S1 Subunit Protein (RBD)

Recombinant SARS-CoV-2, S1 Subunit Protein (RBD) with C-terminal His tag, derived from the transfected human HEK293 cells.

$312.90  
230-30162-100
+

Product Specifications

Size:
100 ug, 500 ug, or 1000 ug
Format:
Liquid
Formulation:
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
Purification:
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration:
Lot specific (see the label on the vial), determined by BCA protein assay
Purity:
>90 %
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Lateral flow, indirect ELISA, sandwich ELISA, glycosylation analysis, binding assay, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, and vaccine development.
Sandwich ELISA: this protein functions as a standard in conjunction with 130-10815 (capture antibody) and 130-10814 (detection antibody).
Standard curve

Source

Species:
SARS-CoV-2
Accession Number:
  • QHD43416
Expressed Region:
Arg319-Phe541
Protein Name & Synonyms:
Spike protein, S Protein, S1 Subunit, Host Cell Receptor Binding Domain (RBD)

Preparation

Expression System:
Human Embryonic Kidney 293 (HEK293) Cells
Conjugation/Tag:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of 25 kDa. Due to the abundant glycosylation, it migrates as approximately 30 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
SDS-PAGE Image
SDS-PAGE
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions.
Binding Function
Binding Function
Product binding ability was measured by ELISA. The immobilized recombinant human ACE2 protein (Catalog #230-30165, coated at 0.5 ug/ml, 100 ul/well) was incubated with the serial diluted SARS CoV-2 S1 RBD wild type protein (Catalog #230-30162). The bound mutant protein was detected by mouse anti-S1 RBD monoclonal antibody using ELISA. The calculated EC50 is 32.77 ng/ml.

Stability & Storage

Shipping
Ice packs
Storage/Stability
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

Spike Protein, S1 Subunit, Host Cell RBD
Spike Protein, S2 Subunit
Nucleocapsid Protein
S1 Subunit Protein (RBD) with C-terminal Fc tag
S1 Subunit Protein (RBD) with C-terminal His-tag
S1 Subunit Protein with C-terminal His-tag
S2 Subunit Protein with C-terminal His-tag
Nucleocapsid Protein with C-terminal His-tag
  1. C. D. Camell et al., Science 373, eabe4832 (2021). DOI: 10.1126/science.abe4832
    Species:
    Sample type:
  2. Beltr?n-Pavez C, et al. Insights into neutralizing antibody responses in individuals exposed to SARS-CoV-2 in Chile. Science Advances (2021).
    Species:
    Sample type:
  3. Borchers C, et al. Evaluation of SARS-CoV-2 spike S1 protein response on PI3K-mediated IL-8 release. Med Sci (Basel) 18;9(2):30 (2021).
    Species:
    Sample type:
  4. Keshavarz B, et al. Quantitative measurement of IgG to severe acute respiratory syndrome coronavirus-2 proteins using immunoCAP. Int Arch Allergy Immunol. 182(5):417-424 (2021).
    Species:
    Sample type:
  5. Miller CJ, et al. FN3-based monobodies selective for the receptor binding domain of the SARS-CoV-2 spike protein. N Biotechnol (2021).
    Species:
    Sample type:
  6. Pirovano, G., Ordonez, A. A., Jain, S. K., Reiner, T., Carroll, L. S., & Pillarsetty, N. V. K. (2021). Rapid detection of SARS-CoV-2 using a radiolabeled antibody. Nuclear Medicine and Biology, 98-99, 69?75. doi:10.1016/j.nucmedbio.2021.05.0
    Species:
    Sample type:
  7. A Antonopoulos, et al. Site-specific characterisation of SARS-CoV-2 spike glyocprotein receptor binding domain. Glycobiology, cwaa085 (2020).
    Species:
    Sample type:
  8. Huang W-C., Zhou S., He X., et al. SARS-CoV-2 RBD Neutralizing Antibody Induction is Enhanced by Particulate Vaccination. Adv. Mater. 2020, 2005637
    Species: Human
    Sample type: Conditioned Media (Liposomes coated with RBD antigen)
  9. Luetkens T, Metcalf R, Planelles V, Zheng Y, Larragoite ET, Spivak ES, Spivak AM, Steinbach M, Blaylock RC, Avila SV, Hankey KG, Martins TB, Slev PR, Mannuel HD, Sajadi M, Rapoport AP, Atanackovic D. Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19. Blood Adv. 2020 Oct 13;4(19):4864-4868. doi: 10.1182/bloodadvances.2020002595. PMID: 33031540; PMCID: PMC7556131.
    Species: Human
    Sample type: Plasma
We emploied this protein for site-specific glycosylation analysis. It works great.

University of California, Davis
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Usage

This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.