Recombinant Human Angiotensin-converting Enzyme 2 (ACE2)

Recombinant Human Angiotensin-converting Enzyme 2 (ACE2) with C-terminal His-tag, derived from the transfected human HEK293 cells.


Product Specifications

100 ug, 500 ug, or 1000 ug
Supplied as a 0.2 um filtered solution in PBS (pH 7.4)
His-tag affinity purification by immobilized metal ion affinity chromatography (IMAC)
Concentration (lot specific):
Lot specific (see the label on the vial), determined by BCA protein assay
>95 %
Purity determined by:
SDS-PAGE under reducing conditions and visualized by Coomassie blue staining
Recommended Applications:
Binding assay, enzyme activity assay, ELISA, glycosylation analysis, antibody generation, hybridoma screening, western blotting, biotin/dye/bead conjugation, binder selection, crystallization, etc.


Accession Number:
  • Q9BYF1
Expressed Region:
Gln18-Ser740 (Extracellular domain)
Protein Name & Synonyms:
ACE2, Angiotensin-converting enzyme 2, ACE-related carboxypeptidase, ACEH, Metalloprotease MPROT15


Expression System:
Human embryonic kidney 293 (HEK293) cells
Conjugation/Tag Notes:
C-terminal his-tag
Molecular Weight (kDa):
Recombinant protein product has a calculated molecular mass of ∼83 kDa. Due to the abundant glycosylation, it migrates as approximately 90 kDa protein bands in SDS-PAGE under DTT, beta-mercaptoethanol reducing conditions. See deglycosylation analysis image below.
Figure 1. Deglycosylation of purified recombinant proteins. Purified proteins were untreated (Lane 2) or treated with Protein Deglycosylation Kit under native (Lane 3) or reducing (Lane 4) conditions. Deglycosylation treatment resulted in a mobility shift of the protein to produce one major band at the expected size, thus indicating that the untreated recombinant protein (Lane 2) was glycosylated.
Lane 1: Protein standard ladder (kDa)
Lane 2: Untreated protein under reducing conditions
Lane 3: Treated protein with deglycosylation enzymes under native conditions
Lane 4: Treated protein with deglycosylation enzymes under reducing conditions
Binding Function
Binding Function
The product S1 RBD (Cat. 230-30162) was coated in 96 well plate and incubated with the serial diluted human ACE2 protein (Raybio, Cat. 230-30165). The bound ACE2 was detected by anti-ACE2 antibody using ELISA. The calculated EC50 is 4.23-9.26 ng/mL.

Stability & Storage

Ice packs
Upon arrival, the protein may be stored for 2 weeks at 4 °C. For long term storage, it is recommended to store at -20 °C or -80 °C in appropriate aliquots. Avoid repeated freeze-thaw cycles.

Related Coronavirus Proteins

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Spike Protein, S2 Subunit
Nucleocapsid Protein
S1 Subunit Protein (RBD) with C-terminal Fc tag
S1 Subunit Protein (RBD) with C-terminal His-tag
S1 Subunit Protein with C-terminal His-tag
S2 Subunit Protein with C-terminal His-tag
Nucleocapsid Protein with C-terminal His-tag

Custom Labeling Request

We can custom label this protein with biotin or fluorescent dyes (choose from violet, blue, green or red). Request now.

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This product is furnished for LABORATORY RESEARCH USE ONLY.
Not for diagnostic or therapeutic use.